Restriction map for
pFN21AB6525
(Circular) (Six-base) MAPSORT of: pFN21AB6525.seq Check: 1172 from: 1 to: 5800
pFN21AB6525 5800 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 3863 5247 5515 1632
Size: 1013 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 1013 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2384 1166
Size: 1218 4582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2179 2350 2945 3543 5115 2179
Size: 171 595 598 1572 2864
Fragments arranged by size:
2864 1572 598 595 171
ApaI G_GGCC'C
Cuts at: 2168 2372 2168
Size: 204 5596
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2428 3109 3335 3487 5596 1493
Size: 935 681 226 152 2109 1697
Fragments arranged by size:
2109 1697 935 681 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 2468 3125 1870
Size: 73 185 340 657 4545
Fragments arranged by size:
4545 657 340 185 73
BamHI G'GATC_C
Cuts at: 2768 3130 3497 2768
Size: 362 367 5071
Fragments arranged by size:
5071 367 362
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 2447 2732 3121 4576
Size: 325 217 627 599 61 285 389 1455
Cuts at: 4576 618
Size: 1842
Fragments arranged by size:
1842 1455 627 599 389 325 285 217
61
BanII G_rGCy'C
Cuts at: 1391 2043 2168 2214 2372 2529 2610 2959 3119
Size: 652 125 46 158 157 81 349 160
Cuts at: 3119 1391
Size: 4072
Fragments arranged by size:
4072 652 349 160 158 157 125 81
46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4829
BbvCI CC'TCA_GC
Cuts at: 2022 2022
Size: 5800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 2355 4411
Size: 107 122 71 631 605 527 156 2056
Cuts at: 4411 136
Size: 1525
Fragments arranged by size:
2056 1525 631 605 527 156 122 107
71
BglII A'GATC_T
Cuts at: 2272 5795 2272
Size: 3523 2277
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2372 2391 2450 3861 5017 1790
Size: 378 204 19 59 1411 1156 2573
Fragments arranged by size:
2573 1411 1156 378 204 59 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2375 3531 4489
Size: 416 53 83 399 24 958 1156 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1156 958 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2594 2615 2865 4460 1239
Size: 1355 21 250 1595 2579
Fragments arranged by size:
2579 1595 1355 250 21
Bpu10I CC'TnA_GC
Cuts at: 2022 2180 2022
Size: 158 5642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 3925 4790 5088 2423
Size: 1502 865 298 3135
Fragments arranged by size:
3135 1502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4463 882
Size: 617 759 2205 2219
Fragments arranged by size:
2219 2205 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2689 4233 4631 4905 5052 5463 2689
Size: 1544 398 274 147 411 3026
Fragments arranged by size:
3026 1544 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2211 2241 1178
Size: 30 165 30 808 30 4737
Fragments arranged by size:
4737 808 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2432 2967 2992 1186
Size: 1246 535 25 3994
Fragments arranged by size:
3994 1246 535 25
BseYI C'CCAG_C
Cuts at: 2035 2161 2353 2636 5003 5499 2035
Size: 126 192 283 2367 496 2336
Fragments arranged by size:
2367 2336 496 283 192 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2855 1163
Size: 573 1119 4108
Fragments arranged by size:
4108 1119 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 2571 2817 2901 2959 3861 3946 5017 2529
Size: 42 246 84 58 902 85 1071 3312
Fragments arranged by size:
3312 1071 902 246 85 84 58 42
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
BsmBI CGTCTCn'nnnn_
Cuts at: 3009 3009
Size: 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2168 2214 2372 2391 2450 2529
Size: 399 253 125 46 158 19 59 79
Cuts at: 2529 2571 2610 2817 2901 2959 3119 3861 3946
Size: 42 39 207 84 58 160 742 85
Cuts at: 3946 5017 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 253 207 160 158
125 85 84 79 59 58 46 42
39 19
BspEI T'CCGG_A
Cuts at: 2689 5463 2689
Size: 2774 3026
BspHI T'CATG_A
Cuts at: 2424 3689 5419 2424
Size: 1265 1730 2805
Fragments arranged by size:
2805 1730 1265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2672 3155 844
Size: 1828 483 3489
Fragments arranged by size:
3489 1828 483
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2735 4444 4631 1700
Size: 110 925 1709 187 2869
Fragments arranged by size:
2869 1709 925 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 2900 3860 4872 2900
Size: 960 1012 3828
Fragments arranged by size:
3828 1012 960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2350 2560 794
Size: 1556 210 4034
Fragments arranged by size:
4034 1556 210
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2997 2997
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2272 2768 3000 3130 3497 3882 3899 5351
Size: 300 496 232 130 367 385 17 1452
Cuts at: 5351 5437 5449 5795 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 496 385 367 346 300 232
130 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2861 2861
Size: 5800
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 2109 2584 513
Size: 552 939 11 94 475 3729
Fragments arranged by size:
3729 939 552 475 94 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2823 487
Size: 683 56 1597 3464
Fragments arranged by size:
3464 1597 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3026 4807 809
Size: 507 1710 1781 1802
Fragments arranged by size:
1802 1781 1710 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2514 2724 2737
Size: 54 1425 50 275 18 684 210 13
Cuts at: 2737 3247 4136 5765 8
Size: 510 889 1629 43
Fragments arranged by size:
1629 1425 889 684 510 275 210 54
50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3730 1353
Size: 2377 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2222 4387 4761 4907 387
Size: 1064 138 633 2165 374 146 1280
Fragments arranged by size:
2165 1280 1064 633 374 146 138
EcoICRI GAG'CTC
Cuts at: 2527 2957 2527
Size: 430 5370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2594 2615 2645 2865 3863 4460 5247
Size: 393 962 21 30 220 998 597 787
Cuts at: 5247 5515 1239
Size: 268 1524
Fragments arranged by size:
1524 998 962 787 597 393 268 220
30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2441 2441
Size: 5800
EcoO109I rG'GnC_Cy
Cuts at: 2011 2330 2347 2369 2473 3028 2011
Size: 319 17 22 104 555 4783
Fragments arranged by size:
4783 555 319 104 22 17
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2036 2673 4947 1678
Size: 358 637 2274 2531
Fragments arranged by size:
2531 2274 637 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2211 2243 2257 2289 2366
Size: 32 135 32 639 32 14 32 77
Cuts at: 2366 2398 2861 2893 4441 4473 4515 4547 1373
Size: 32 463 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1548 639 463 135 77 42 32
32 32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2304 2313 748
Size: 1556 9 4235
Fragments arranged by size:
4235 1556 9
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 3758 4117 4276 518
Size: 648 1218 1374 359 159 2042
Fragments arranged by size:
2042 1374 1218 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2111 2491 2586 2729 2942 3216 3893
Size: 411 94 380 95 143 213 274 677
Cuts at: 3893 5041 5286 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 411 380 274 245 213
143 95 94
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2028 3044 1348
Size: 680 1016 4104
Fragments arranged by size:
4104 1016 680
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2330 3028 2011
Size: 319 698 4783
Fragments arranged by size:
4783 698 319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2164 2368 2164
Size: 204 5596
PstI C_TGCA'G
Cuts at: 830 2346 2669 3152 830
Size: 1516 323 483 3478
Fragments arranged by size:
3478 1516 483 323
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SacI G_AGCT'C
Cuts at: 2529 2959 2529
Size: 430 5370
SacII CC_GC'GG
Cuts at: 2018 2112 2587 2018
Size: 94 475 5231
Fragments arranged by size:
5231 475 94
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SanDI GG'GwC_CC
Cuts at: 2011 2011
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SexAI A'CCwGG_T
Cuts at: 2238 2238
Size: 5800
SfcI C'TryA_G
Cuts at: 826 1045 2342 2665 2946 3148 4283 4964 5155
Size: 219 1297 323 281 202 1135 681 191
Cuts at: 5155 826
Size: 1471
Fragments arranged by size:
1471 1297 1135 681 323 281 219 202
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 2130 3127 1872
Size: 258 997 4545
Fragments arranged by size:
4545 997 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 3904 4805 5067 820
Size: 197 926 495 1466 901 262 1553
Fragments arranged by size:
1553 1466 926 901 495 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 2004 2996 3239 513
Size: 552 939 992 243 3074
Fragments arranged by size:
3074 992 939 552 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 3827 3844 3997 4182 4627 325
Size: 1255 557 1690 17 153 185 445 1498
Fragments arranged by size:
1690 1498 1255 557 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4046 5758 96
Size: 274 80 33 51 1443 2069 1712 138
Fragments arranged by size:
2069 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2965 2997 5760 5792 2965
Size: 32 2763 32 2973
Fragments arranged by size:
2973 2763 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 2128 3125 1870
Size: 258 997 4545
Fragments arranged by size:
4545 997 258
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI
SwaI XcmI