Restriction map for
pFN21AB6523
(Circular) (Six-base) MAPSORT of: pFN21AB6523.seq Check: 8137 from: 1 to: 5797
pFN21AB6523 5797 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3045 3140 1937
Size: 1108 95 4594
Fragments arranged by size:
4594 1108 95
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2703 3860 5244 5512 1632
Size: 1071 1157 1384 268 1917
Fragments arranged by size:
1917 1384 1157 1071 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2929 4696 5673 1203
Size: 1726 1767 977 1327
Fragments arranged by size:
1767 1726 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 2555 1166
Size: 1389 4408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaI G_GGCC'C
Cuts at: 2181 2181
Size: 5797
ApaLI G'TGCA_C
Cuts at: 2933 3854 5010 2933
Size: 921 1156 3720
Fragments arranged by size:
3720 1156 921
ApoI r'AATT_y
Cuts at: 1493 2276 3106 3332 3484 5593 1493
Size: 783 830 226 152 2109 1697
Fragments arranged by size:
2109 1697 830 783 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 4573 618
Size: 325 217 627 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 2181 3116 1391
Size: 751 39 935 4072
Fragments arranged by size:
4072 935 751 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 928
Size: 971 815 4011
Fragments arranged by size:
4011 971 815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 2559 1548
Size: 1011 4786
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2503 5792 2503
Size: 3289 2508
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2181 2326 2937 3858 5014 1790
Size: 391 145 611 921 1156 2573
Fragments arranged by size:
2573 1156 921 611 391 145
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2562 3528 4486
Size: 416 53 83 399 24 1145 966 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1145 966 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2807 2943 4457 1239
Size: 806 762 136 1514 2579
Fragments arranged by size:
2579 1514 806 762 136
Bpu10I CC'TnA_GC
Cuts at: 2034 2250 2034
Size: 216 5581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2972 3922 4787 5085 2972
Size: 950 865 298 3684
Fragments arranged by size:
3684 950 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2624 2654 2916 2946 1178
Size: 30 165 30 1221 30 262 30 4029
Fragments arranged by size:
4029 1221 262 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 2237 2240 2243 2642 2645 1186
Size: 1048 3 3 3 399 3 4338
Fragments arranged by size:
4338 1048 399 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2047 2168 5000 5496 2047
Size: 121 2832 496 2348
Fragments arranged by size:
2832 2348 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3029 1163
Size: 573 1293 3931
Fragments arranged by size:
3931 1293 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3247 4008 5036 1540
Size: 275 186 1054 192 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 192 186
BsiHKAI G_wGCw'C
Cuts at: 2937 3858 3943 5014 2937
Size: 921 85 1071 3720
Fragments arranged by size:
3720 1071 921 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
BsmBI CGTCTCn'nnnn_
Cuts at: 3012 3012
Size: 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2181 2326 2937 3116 3858 3943
Size: 399 352 39 145 611 179 742 85
Cuts at: 3943 5014 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 611 399 352 179 145
85 39
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3152 844
Size: 2308 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2530 4292 4466 65
Size: 1183 1282 1762 174 1396
Fragments arranged by size:
1762 1396 1282 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3050 4441 4628 1700
Size: 110 1240 1391 187 2869
Fragments arranged by size:
2869 1391 1240 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstXI CCAn_nnnn'nTGG
Cuts at: 2150 2150
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 2433 2503 2948 3127 3494 3879 3896 5348
Size: 461 70 445 179 367 385 17 1452
Cuts at: 5348 5434 5446 5792 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 461 445 385 367 346 179
86 70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2166 3313 4107 4127 2166
Size: 1147 794 20 3836
Fragments arranged by size:
3836 1147 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DraIII CAC_nnn'GTG
Cuts at: 2941 2941
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2643 3052 3244
Size: 54 1425 50 275 18 813 409 192
Cuts at: 3244 4133 5762 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 813 409 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 3244 1537
Size: 275 1240 192 4090
Fragments arranged by size:
4090 1240 275 192
EarI CTCTTCn'nnn_
Cuts at: 1353 2196 2202 2211 3727 1353
Size: 843 6 9 1516 3423
Fragments arranged by size:
3423 1516 843 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2703 2807 2943 3860 4457 5244
Size: 393 413 658 104 136 917 597 787
Cuts at: 5244 5512 1239
Size: 268 1524
Fragments arranged by size:
1524 917 787 658 597 413 393 268
136 104
EcoNI CCTnn'n_nnAGG
Cuts at: 2789 2789
Size: 5797
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 5797
FspI TGC'GCA
Cuts at: 2549 4303 2549
Size: 1754 4043
HaeII r_GCGC'y
Cuts at: 1678 4944 1678
Size: 3266 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2808 2840 4438
Size: 32 135 32 1052 32 152 32 1598
Cuts at: 4438 4470 4512 4544 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1598 1052 152 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 3141 3387 669
Size: 988 281 322 881 246 3079
Fragments arranged by size:
3079 988 881 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2863 4911 5095 1305
Size: 1558 2048 184 2007
Fragments arranged by size:
2048 2007 1558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3975
Fragments arranged by size:
3975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 3755 4114 4273 518
Size: 648 1389 1200 359 159 2042
Fragments arranged by size:
2042 1389 1200 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 3213 3890 5038 5283 1606
Size: 471 1136 677 1148 245 2120
Fragments arranged by size:
2120 1148 1136 677 471 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3004 1108
Size: 1896 3901
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2177 2177
Size: 5797
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SexAI A'CCwGG_T
Cuts at: 2440 2440
Size: 5797
SfcI C'TryA_G
Cuts at: 826 1045 2072 2106 2458 2473 2610 3145 4280
Size: 219 1027 34 352 15 137 535 1135
Cuts at: 4280 4961 5152 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 535 352 219 191
137 34 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2987 3901 4802 5064 820
Size: 197 926 1044 914 901 262 1553
Fragments arranged by size:
1553 1044 926 914 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 3236 513
Size: 552 2171 3074
Fragments arranged by size:
3074 2171 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2384 2416 2430 2462 2916 2948 5757 5789 2384
Size: 32 14 32 454 32 2809 32 2392
Fragments arranged by size:
2809 2392 454 32 32 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3085 3133 3085
Size: 48 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI