Restriction map for
pFN21AB6522
(Circular) (Six-base) MAPSORT of: pFN21AB6522.seq Check: 6223 from: 1 to: 5866
pFN21AB6522 5866 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2005 3174 3929 5313 5581 1632
Size: 373 1169 755 1384 268 1917
Fragments arranged by size:
1917 1384 1169 755 373 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 2760 2930 3609 5181 2760
Size: 170 679 1572 3445
Fragments arranged by size:
3445 1572 679 170
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 2189 2204 2372 2427 2605 2631 3175 3401
Size: 696 15 168 55 178 26 544 226
Cuts at: 3401 3553 5662 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 696 544 226 178 168 152
55 26 15
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 1870
Size: 73 1248 4545
Fragments arranged by size:
4545 1248 73
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3187 4642 618
Size: 325 217 627 1400 1455 1842
Fragments arranged by size:
1842 1455 1400 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3185 1391
Size: 1794 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2424 928
Size: 971 525 4370
Fragments arranged by size:
4370 971 525
BbvCI CC'TCA_GC
Cuts at: 3063 3063
Size: 5866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 2392 1548
Size: 844 5022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4477 136
Size: 107 122 71 631 605 2805 1525
Fragments arranged by size:
2805 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 2489 3283 2489
Size: 794 5072
Bme1580I G_kGCm'C
Cuts at: 1790 3927 5083 1790
Size: 2137 1156 2573
Fragments arranged by size:
2573 2137 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 4526 1239
Size: 943 2344 2579
Fragments arranged by size:
2579 2344 943
Bpu10I CC'TnA_GC
Cuts at: 3063 3063
Size: 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2862 4529 882
Size: 617 1363 1667 2219
Fragments arranged by size:
2219 1667 1363 617
BsaAI yAC'GTr
Cuts at: 493 1204 2157 2827 3102 3657 493
Size: 711 953 670 275 555 2702
Fragments arranged by size:
2702 953 711 670 555 275
BsaBI GATnn'nnATC
Cuts at: 2931 5860 2931
Size: 2929 2937
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4299 4697 4971 5118 5529 4299
Size: 398 274 147 411 4636
Fragments arranged by size:
4636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 1178
Size: 30 165 30 597 30 5014
Fragments arranged by size:
5014 597 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2550 2570 2960 3080 1186
Size: 1364 20 390 120 3972
Fragments arranged by size:
3972 1364 390 120 20
BseYI C'CCAG_C
Cuts at: 5069 5565 5069
Size: 496 5370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2424 1163
Size: 573 688 4605
Fragments arranged by size:
4605 688 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3927 4012 5083 3927
Size: 85 1071 4710
Fragments arranged by size:
4710 1071 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
BsmBI CGTCTCn'nnnn_
Cuts at: 2212 2212
Size: 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3185 3927 4012 5083 1391
Size: 399 1395 742 85 1071 2174
Fragments arranged by size:
2174 1395 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3221 844
Size: 2377 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2288 4361 4535 65
Size: 1183 1040 2073 174 1396
Fragments arranged by size:
2073 1396 1183 1040 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 2815 96
Size: 2719 3147
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 2181 3926 4938 2181
Size: 1745 1012 3109
Fragments arranged by size:
3109 1745 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5866
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2095 2713 3196 3563 3948 3965 5417 5503
Size: 123 618 483 367 385 17 1452 86
Cuts at: 5503 5515 5861 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 618 483 385 367 346 123
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 2436 3382 4176 4196 2436
Size: 946 794 20 4106
Fragments arranged by size:
4106 946 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 2091 3170 2091
Size: 1079 4787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3313 4202 5831
Size: 54 1425 50 275 18 1483 889 1629
Cuts at: 5831 8
Size: 43
Fragments arranged by size:
1629 1483 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2464 2703 2860 3796 1353
Size: 752 359 239 157 936 3423
Fragments arranged by size:
3423 936 752 359 239 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2182 3174 3929 4526 5313 5581
Size: 393 373 177 992 755 597 787 268
Cuts at: 5581 1239
Size: 1524
Fragments arranged by size:
1524 992 787 755 597 393 373 268
177
EcoO109I rG'GnC_Cy
Cuts at: 2477 2800 2477
Size: 323 5543
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
EcoRV GAT'ATC
Cuts at: 2225 3037 2225
Size: 812 5054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2252 2116
Size: 136 5730
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 5013 1678
Size: 3335 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2209 2241 2606
Size: 32 135 32 428 32 177 32 365
Cuts at: 2606 2638 4507 4539 4581 4613 1373
Size: 32 1869 32 42 32 2626
Fragments arranged by size:
2626 1869 428 365 177 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2445 748
Size: 1697 4169
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2423 4980 5164 1305
Size: 1118 2557 184 2007
Fragments arranged by size:
2557 2007 1118 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4044
Fragments arranged by size:
4044 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2567 3081 3824 4183 4342 518
Size: 648 843 558 514 743 359 159 2042
Fragments arranged by size:
2042 843 743 648 558 514 359 159
MspA1I CmG'CkG
Cuts at: 1606 2745 3282 3959 5107 5352 1606
Size: 1139 537 677 1148 245 2120
Fragments arranged by size:
2120 1148 1139 677 537 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 3224 4769 1132
Size: 97 1995 1545 2229
Fragments arranged by size:
2229 1995 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2477 2477
Size: 5866
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 2637 3436 2637
Size: 799 5067
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2745 2745
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 3214 4349 5030 5221 826
Size: 219 2169 1135 681 191 1471
Fragments arranged by size:
2169 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3970 4871 5133 820
Size: 197 926 2027 901 262 1553
Fragments arranged by size:
2027 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2157 3657 493
Size: 1664 1500 2702
Fragments arranged by size:
2702 1664 1500
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 2654 3790 5
Size: 47 2602 1136 2081
Fragments arranged by size:
2602 2081 1136 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2004 3305 513
Size: 552 939 1301 3074
Fragments arranged by size:
3074 1301 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2840 3893 3910 4063 4248 4693 325
Size: 1255 1260 1053 17 153 185 445 1498
Fragments arranged by size:
1498 1260 1255 1053 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2815 4112 5824
Size: 274 80 33 51 1443 838 1297 1712
Cuts at: 5824 96
Size: 138
Fragments arranged by size:
1712 1443 1297 838 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3136 3136
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 2018 3995 2018
Size: 1977 3889
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI