Restriction map for
pFN21AB6521
(Circular) (Six-base) MAPSORT of: pFN21AB6521.seq Check: 9561 from: 1 to: 5794
pFN21AB6521 5794 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3857 5241 5509 1632
Size: 2225 1384 268 1917
Fragments arranged by size:
2225 1917 1384 268
AfeI AGC'GCT
Cuts at: 2746 2746
Size: 5794
AflII C'TTAA_G
Cuts at: 820 1017 2716 820
Size: 197 1699 3898
Fragments arranged by size:
3898 1699 197
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5794
AlwNI CAG_nnn'CTG
Cuts at: 3537 5109 3537
Size: 1572 4222
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2377 2591 2619 3103 3329 3481 5590 1493
Size: 884 214 28 484 226 152 2109 1697
Fragments arranged by size:
2109 1697 884 484 226 214 152 28
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 1870
Size: 73 1176 4545
Fragments arranged by size:
4545 1176 73
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3115 4570 618
Size: 325 217 627 1328 1455 1842
Fragments arranged by size:
1842 1455 1328 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3113 1391
Size: 1722 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4823
BbvCI CC'TCA_GC
Cuts at: 2473 2473
Size: 5794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3985 4019 1903
Size: 34 50 34 1964 34 3678
Fragments arranged by size:
3678 1964 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BfrBI ATG'CAT
Cuts at: 2333 2333
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2212 5789 2212
Size: 3577 2217
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 3855 5011 1790
Size: 2065 1156 2573
Fragments arranged by size:
2573 2065 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 4483 442
Size: 416 53 83 399 24 2108 958 1753
Fragments arranged by size:
2108 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4454 1239
Size: 3215 2579
Bpu10I CC'TnA_GC
Cuts at: 2473 2481 2473
Size: 8 5786
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 3919 4784 5082 2342
Size: 1577 865 298 3054
Fragments arranged by size:
3054 1577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4457 882
Size: 617 2958 2219
Fragments arranged by size:
2958 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3601 3983 4618 275
Size: 53 83 186 936 2068 382 635 1451
Fragments arranged by size:
2068 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4227 4625 4899 5046 5457 4227
Size: 398 274 147 411 4564
Fragments arranged by size:
4564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2498 2528 1178
Size: 30 165 30 1095 30 4444
Fragments arranged by size:
4444 1095 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2079 1186
Size: 893 4901
BseYI C'CCAG_C
Cuts at: 2801 4997 5493 2801
Size: 2196 496 3102
Fragments arranged by size:
3102 2196 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3244 4005 5033 1540
Size: 275 186 1243 761 1028 2301
Fragments arranged by size:
2301 1243 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3855 3940 5011 3855
Size: 85 1071 4638
Fragments arranged by size:
4638 1071 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3113 3855 3940 5011 1391
Size: 399 1323 742 85 1071 2174
Fragments arranged by size:
2174 1323 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3149 844
Size: 2305 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3681 1394
Size: 429 1858 3507
Fragments arranged by size:
3507 1858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2643 2796 4289 4463 65
Size: 1183 1395 153 1493 174 1396
Fragments arranged by size:
1493 1396 1395 1183 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 4438 4625 1700
Size: 110 2628 187 2869
Fragments arranged by size:
2869 2628 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 3854 4866 3854
Size: 1012 4782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3026 794
Size: 2232 3562
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2269
Size: 5794
BstYI r'GATC_y
Cuts at: 1972 2212 3124 3491 3876 3893 5345 5431 5443
Size: 240 912 367 385 17 1452 86 12
Cuts at: 5443 5789 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 912 385 367 346 240 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2502 513
Size: 552 1437 3805
Fragments arranged by size:
3805 1437 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2190 3310 4104 4124 2190
Size: 1120 794 20 3860
Fragments arranged by size:
3860 1120 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2130 2165 529
Size: 35 1566 35 4158
Fragments arranged by size:
4158 1566 35 35
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 809
Size: 507 3485 1802
Fragments arranged by size:
3485 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3241 4130 5759
Size: 54 1425 50 275 18 1411 889 1629
Cuts at: 5759 8
Size: 43
Fragments arranged by size:
1629 1425 1411 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3241 1537
Size: 275 1429 4090
Fragments arranged by size:
4090 1429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3724 1353
Size: 2371 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2418 4381 4755 4901 387
Size: 1064 138 829 1963 374 146 1280
Fragments arranged by size:
1963 1280 1064 829 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3857 4454 5241 5509 1239
Size: 393 2225 597 787 268 1524
Fragments arranged by size:
2225 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2033 2033
Size: 5794
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 2748 4941 1678
Size: 1070 2193 2531
Fragments arranged by size:
2531 2193 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2498 2530 4435
Size: 32 135 32 472 32 422 32 1905
Cuts at: 4435 4467 4509 4541 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1905 472 422 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3007 3138 3384 669
Size: 988 281 1069 131 246 3079
Fragments arranged by size:
3079 1069 988 281 246 131
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4908 5092 1305
Size: 3603 184 2007
Fragments arranged by size:
3603 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3972
Fragments arranged by size:
3972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3752 4111 4270 518
Size: 648 2586 359 159 2042
Fragments arranged by size:
2586 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2477 2816 3210 3887 5035 5280 1606
Size: 444 427 339 394 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 427 394 339 245
NcoI C'CATG_G
Cuts at: 513 1065 2502 513
Size: 552 1437 3805
Fragments arranged by size:
3805 1437 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NsiI A_TGCA'T
Cuts at: 2335 2335
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 3152 4697 1132
Size: 97 1923 1545 2229
Fragments arranged by size:
2229 1923 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2121 1348
Size: 773 5021
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2941 1108
Size: 1833 3961
PfoI T'CCnGG_A
Cuts at: 2166 2166
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2334 2366 2334
Size: 32 5762
PstI C_TGCA'G
Cuts at: 830 3146 830
Size: 2316 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
PvuII CAG'CTG
Cuts at: 2477 2816 2477
Size: 339 5455
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 2038 4042 5754 2038
Size: 2004 1712 2078
Fragments arranged by size:
2078 2004 1712
SfcI C'TryA_G
Cuts at: 826 1045 3142 4277 4958 5149 826
Size: 219 2097 1135 681 191 1471
Fragments arranged by size:
2097 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 2716 3898 4799 5061 820
Size: 197 926 378 395 1182 901 262 1553
Fragments arranged by size:
1553 1182 926 901 395 378 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5794
StyI C'CwwG_G
Cuts at: 513 1065 2055 2502 3233 513
Size: 552 990 447 731 3074
Fragments arranged by size:
3074 990 731 552 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2113 3821 3838 3991 4176 4621 325
Size: 1255 533 1708 17 153 185 445 1498
Fragments arranged by size:
1708 1498 1255 533 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2036 4040 5752
Size: 274 80 33 51 1443 59 2004 1712
Cuts at: 5752 96
Size: 138
Fragments arranged by size:
2004 1712 1443 274 138 80 59 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5754 5786 5754
Size: 32 5762
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 2579 3130 2579
Size: 551 5243
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI