Restriction map for
pFN21AB6520
(Circular) (Six-base) MAPSORT of: pFN21AB6520.seq Check: 9615 from: 1 to: 5863
pFN21AB6520 5863 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2895 3673 278
Size: 53 83 186 936 1359 778 2468
Fragments arranged by size:
2468 1359 936 778 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 1937
Size: 1269 4594
Acc65I G'GTAC_C
Cuts at: 2277 2778 3184 2277
Size: 501 406 4956
Fragments arranged by size:
4956 501 406
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 3926 5310 5578 1632
Size: 1046 1248 1384 268 1917
Fragments arranged by size:
1917 1384 1248 1046 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 2515 2570 4592 2515
Size: 55 2022 3786
Fragments arranged by size:
3786 2022 55
AleI CACnn'nnGTG
Cuts at: 1166 2757 1166
Size: 1591 4272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5863
AlwNI CAG_nnn'CTG
Cuts at: 3006 3606 5178 3006
Size: 600 1572 3691
Fragments arranged by size:
3691 1572 600
ApaI G_GGCC'C
Cuts at: 2841 2841
Size: 5863
ApaLI G'TGCA_C
Cuts at: 2405 3920 5076 2405
Size: 1515 1156 3192
Fragments arranged by size:
3192 1515 1156
ApoI r'AATT_y
Cuts at: 1493 3172 3398 3550 5659 1493
Size: 1679 226 152 2109 1697
Fragments arranged by size:
2109 1697 1679 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2387 2395 2647 2834 3188 1870
Size: 73 153 291 8 252 187 354 4545
Fragments arranged by size:
4545 354 291 252 187 153 73 8
BamHI G'GATC_C
Cuts at: 2066 2948 3193 3560 2066
Size: 882 245 367 4369
Fragments arranged by size:
4369 882 367 245
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2277 2732 2778 3184 4639
Size: 325 217 627 490 455 46 406 1455
Cuts at: 4639 618
Size: 1842
Fragments arranged by size:
1842 1455 627 490 455 406 325 217
46
BanII G_rGCy'C
Cuts at: 1391 2043 2079 2841 3013 3062 3182 1391
Size: 652 36 762 172 49 120 4072
Fragments arranged by size:
4072 762 652 172 120 49 36
BbeI G_GCGC'C
Cuts at: 2736 2736
Size: 5863
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 2601 2889 928
Size: 971 261 441 288 3902
Fragments arranged by size:
3902 971 441 288 261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2337 2371 4054 4088 1903
Size: 34 400 34 1683 34 3678
Fragments arranged by size:
3678 1683 400 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2382 4474
Size: 107 122 71 631 605 337 373 2092
Cuts at: 4474 136
Size: 1525
Fragments arranged by size:
2092 1525 631 605 373 337 122 107
71
BglII A'GATC_T
Cuts at: 2110 5858 2110
Size: 3748 2115
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 2409 2841 3924 5080 1790
Size: 619 432 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 619 432
BmgBI CAC'GTC
Cuts at: 1318 2053 1318
Size: 735 5128
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2431 3016 3594
Size: 416 53 83 399 24 1014 585 578
Cuts at: 3594 4552 442
Size: 958 1753
Fragments arranged by size:
1753 1014 958 585 578 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2930 4523 1239
Size: 926 765 1593 2579
Fragments arranged by size:
2579 1593 926 765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2065 2480 2552 3988 4853 5151 2065
Size: 415 72 1436 865 298 2777
Fragments arranged by size:
2777 1436 865 415 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4526 882
Size: 617 3027 2219
Fragments arranged by size:
3027 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2733 2892 3670 4052
Size: 53 83 186 936 1200 159 778 382
Cuts at: 4052 4687 275
Size: 635 1451
Fragments arranged by size:
1451 1200 936 778 635 382 186 159
83 53
BsaWI w'CCGG_w
Cuts at: 2306 4296 4694 4968 5115 5526 2306
Size: 1990 398 274 147 411 2643
Fragments arranged by size:
2643 1990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5638
Fragments arranged by size:
5638 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2575 2717 1186
Size: 1389 142 4332
Fragments arranged by size:
4332 1389 142
BseYI C'CCAG_C
Cuts at: 2017 2042 2771 2986 5066 5562 2017
Size: 25 729 215 2080 496 2318
Fragments arranged by size:
2318 2080 729 496 215 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2512 3313 4074 5102 1540
Size: 275 186 511 801 761 1028 2301
Fragments arranged by size:
2301 1028 801 761 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2409 3062 3924 4009 5080 2079
Size: 330 653 862 85 1071 2862
Fragments arranged by size:
2862 1071 862 653 330 85
BsmI GAATG_Cn'
Cuts at: 2965 3374 3467 2965
Size: 409 93 5361
Fragments arranged by size:
5361 409 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2306 2306
Size: 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2079 2409 2841 3013 3062 3182
Size: 399 253 36 330 432 172 49 120
Cuts at: 3182 3924 4009 5080 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 330 253 172
120 85 49 36
BspEI T'CCGG_A
Cuts at: 2306 5526 2306
Size: 3220 2643
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3218 844
Size: 2374 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4358 4532 65
Size: 1183 3110 174 1396
Fragments arranged by size:
3110 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2796 4507 4694 1700
Size: 110 986 1711 187 2869
Fragments arranged by size:
2869 1711 986 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2169 2349 794
Size: 1375 180 4308
Fragments arranged by size:
4308 1375 180
BstBI TT'CG_AA
Cuts at: 2541 4667 2541
Size: 2126 3737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 3091 3091
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 2066 2110 2217 2948 3193 3560 3945 3962
Size: 94 44 107 731 245 367 385 17
Cuts at: 3962 5414 5500 5512 5858 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 731 385 367 346 245 107
94 86 44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2941 513
Size: 552 939 937 3435
Fragments arranged by size:
3435 939 937 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 3379 4173 4193 3379
Size: 794 20 5049
Fragments arranged by size:
5049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 4870 809
Size: 507 1575 1979 1802
Fragments arranged by size:
1979 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3072 3310
Size: 54 1425 50 275 18 180 1062 238
Cuts at: 3310 4199 5828 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1062 889 275 238 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 3793 1353
Size: 908 1532 3423
Fragments arranged by size:
3423 1532 908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2961 4450 4824 4970 387
Size: 1064 138 1372 1489 374 146 1280
Fragments arranged by size:
1489 1372 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 3060 2077
Size: 983 4880
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2678 2930 3926 4523 5310 5578
Size: 393 533 513 252 996 597 787 268
Cuts at: 5578 1239
Size: 1524
Fragments arranged by size:
1524 996 787 597 533 513 393 268
252
EcoO109I rG'GnC_Cy
Cuts at: 2391 2711 2838 2391
Size: 320 127 5416
Fragments arranged by size:
5416 320 127
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 2736 5010 1678
Size: 1058 2274 2531
Fragments arranged by size:
2531 2274 1058
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 2556 2588 2882
Size: 32 135 32 929 32 23 32 294
Cuts at: 2882 2914 4504 4536 4578 4610 1373
Size: 32 1590 32 42 32 2626
Fragments arranged by size:
2626 1590 929 294 135 42 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KasI G'GCGC_C
Cuts at: 2732 2732
Size: 5863
KpnI G_GTAC'C
Cuts at: 2281 2782 3188 2281
Size: 501 406 4956
Fragments arranged by size:
4956 501 406
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2453 2499 4977 5161 1305
Size: 1148 46 2478 184 2007
Fragments arranged by size:
2478 2007 1148 184 46
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4041
Fragments arranged by size:
4041 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2757 3821 4180 4339 518
Size: 648 885 706 1064 359 159 2042
Fragments arranged by size:
2042 1064 885 706 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2775 3279 3956 5104 5349 1606
Size: 1169 504 677 1148 245 2120
Fragments arranged by size:
2120 1169 1148 677 504 245
NarI GG'CG_CC
Cuts at: 2733 2733
Size: 5863
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4372
Fragments arranged by size:
4372 939 552
NdeI CA'TA_TG
Cuts at: 387 2122 387
Size: 1735 4128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2666 3221 4766 1132
Size: 97 1437 555 1545 2229
Fragments arranged by size:
2229 1545 1437 555 97
PasI CC'CwG_GG
Cuts at: 1348 2746 1348
Size: 1398 4465
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5863
PfoI T'CCnGG_A
Cuts at: 2244 2907 2244
Size: 663 5200
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2391 2711 2391
Size: 320 5543
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PspOMI G'GGCC_C
Cuts at: 2837 2837
Size: 5863
PstI C_TGCA'G
Cuts at: 830 2469 2808 3215 830
Size: 1639 339 407 3478
Fragments arranged by size:
3478 1639 407 339
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2775 2775
Size: 5863
SacI G_AGCT'C
Cuts at: 2079 3062 2079
Size: 983 4880
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SanDI GG'GwC_CC
Cuts at: 2711 2711
Size: 5863
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SfcI C'TryA_G
Cuts at: 826 1045 2465 2804 3211 4346 5027 5218 826
Size: 219 1420 339 407 1135 681 191 1471
Fragments arranged by size:
1471 1420 1135 681 407 339 219 191
SfoI GGC'GCC
Cuts at: 2734 2734
Size: 5863
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 2098 2389 2649 2836 3190 1872
Size: 226 291 260 187 354 4545
Fragments arranged by size:
4545 354 291 260 226 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 2495 2531 3967 4868 5130
Size: 197 926 137 415 36 1436 901 262
Cuts at: 5130 820
Size: 1553
Fragments arranged by size:
1553 1436 926 901 415 262 197 137
36
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3125 3787 5
Size: 47 3073 662 2081
Fragments arranged by size:
3073 2081 662 47
StuI AGG'CCT
Cuts at: 1517 2501 1517
Size: 984 4879
StyI C'CwwG_G
Cuts at: 513 1065 2004 3302 513
Size: 552 939 1298 3074
Fragments arranged by size:
3074 1298 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2413 2526 3890 3907 4060 4245 4690
Size: 1255 833 113 1364 17 153 185 445
Cuts at: 4690 325
Size: 1498
Fragments arranged by size:
1498 1364 1255 833 445 185 153 113
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4109 5821 96
Size: 274 80 33 51 1443 2132 1712 138
Fragments arranged by size:
2132 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 5823 5855 2933
Size: 32 2858 32 2941
Fragments arranged by size:
2941 2858 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2743 2020
Size: 723 5140
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 2096 2387 2647 2834 3188 1870
Size: 226 291 260 187 354 4545
Fragments arranged by size:
4545 354 291 260 226 187
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2893 3671 276
Size: 53 83 186 936 1359 778 2468
Fragments arranged by size:
2468 1359 936 778 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI