Restriction map for
pFN21AB6518
(Circular) (Six-base) MAPSORT of: pFN21AB6518.seq Check: 3149 from: 1 to: 5863
pFN21AB6518 5863 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2299 2859 2962 2299
Size: 560 103 5200
Fragments arranged by size:
5200 560 103
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 3206 1937
Size: 517 752 4594
Fragments arranged by size:
4594 752 517
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 2201 2822 3926 5310 5578 1632
Size: 487 82 621 1104 1384 268 1917
Fragments arranged by size:
1917 1384 1104 621 487 268 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 2108 1166
Size: 942 4921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2888 1184
Size: 1704 4159
AlwNI CAG_nnn'CTG
Cuts at: 2142 2380 2509 3606 5178 2142
Size: 238 129 1097 1572 2827
Fragments arranged by size:
2827 1572 1097 238 129
ApaI G_GGCC'C
Cuts at: 2098 2098
Size: 5863
ApaLI G'TGCA_C
Cuts at: 3920 5076 3920
Size: 1156 4707
ApoI r'AATT_y
Cuts at: 1493 3172 3398 3550 5659 1493
Size: 1679 226 152 2109 1697
Fragments arranged by size:
2109 1697 1679 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 3188 1870
Size: 73 83 1162 4545
Fragments arranged by size:
4545 1162 83 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2944 3184 4639 618
Size: 325 217 627 1157 240 1455 1842
Fragments arranged by size:
1842 1455 1157 627 325 240 217
BanII G_rGCy'C
Cuts at: 1391 2019 2098 2651 3182 1391
Size: 628 79 553 531 4072
Fragments arranged by size:
4072 628 553 531 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4054 4088 1903
Size: 34 2117 34 3678
Fragments arranged by size:
3678 2117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3041 3056 4474
Size: 107 122 71 631 605 1369 15 1418
Cuts at: 4474 136
Size: 1525
Fragments arranged by size:
1525 1418 1369 631 605 122 107 71
15
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2098 2949 3924 5080 1790
Size: 298 10 851 975 1156 2573
Fragments arranged by size:
2573 1156 975 851 298 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 3082 3106
Size: 416 53 83 399 24 658 1007 24
Cuts at: 3106 3594 4552 442
Size: 488 958 1753
Fragments arranged by size:
1753 1007 958 658 488 416 399 83
53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2300 2807 4523 1239
Size: 805 256 507 1716 2579
Fragments arranged by size:
2579 1716 805 507 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3988 4853 5151 3988
Size: 865 298 4700
Fragments arranged by size:
4700 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2122 2480 2522 4526 882
Size: 617 623 358 42 2004 2219
Fragments arranged by size:
2219 2004 623 617 358 42
BsaAI yAC'GTr
Cuts at: 493 1204 2554 3654 493
Size: 711 1350 1100 2702
Fragments arranged by size:
2702 1350 1100 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2619 3670 4052 4687
Size: 53 83 186 936 1086 1051 382 635
Cuts at: 4687 275
Size: 1451
Fragments arranged by size:
1451 1086 1051 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2157 4296 4694 4968 5115 5526 2157
Size: 2139 398 274 147 411 2494
Fragments arranged by size:
2494 2139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5638
Fragments arranged by size:
5638 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2288 2422 2723 1186
Size: 931 171 134 301 4326
Fragments arranged by size:
4326 931 301 171 134
BseYI C'CCAG_C
Cuts at: 2233 2560 3004 5066 5562 2233
Size: 327 444 2062 496 2534
Fragments arranged by size:
2534 2062 496 444 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 2693 2990 1163
Size: 573 543 414 297 4036
Fragments arranged by size:
4036 573 543 414 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2602 2763 3048 3313 4074 5102
Size: 275 186 601 161 285 265 761 1028
Cuts at: 5102 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 601 285 275 265 186
161
BsiHKAI G_wGCw'C
Cuts at: 2651 3924 4009 5080 2651
Size: 1273 85 1071 3434
Fragments arranged by size:
3434 1273 1071 85
BsmI GAATG_Cn'
Cuts at: 2388 3374 3467 2388
Size: 986 93 4784
Fragments arranged by size:
4784 986 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2088 2098 2651 2949 3182 3924
Size: 399 229 69 10 553 298 233 742
Cuts at: 3924 4009 5080 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 399 298 233 229
85 69 10
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 2466 2859 2962 3218 844
Size: 1455 167 393 103 256 3489
Fragments arranged by size:
3489 1455 393 256 167 103
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 2620 3105 4358 4532 65
Size: 1183 1158 214 485 1253 174 1396
Fragments arranged by size:
1396 1253 1183 1158 485 214 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3050 4507 4694 1700
Size: 110 1240 1457 187 2869
Fragments arranged by size:
2869 1457 1240 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3124 794
Size: 2330 3533
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2113
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 2419 2830 3193 3560 3945 3962 5414 5500
Size: 447 411 363 367 385 17 1452 86
Cuts at: 5500 5512 5858 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 447 411 385 367 363 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2074 2652 2702 2757 3036 3095 513
Size: 552 1009 578 50 55 279 59 3281
Fragments arranged by size:
3281 1009 578 552 279 59 55 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2022 487
Size: 683 56 796 4328
Fragments arranged by size:
4328 796 683 56
BtsI GCAGTG_nn'
Cuts at: 3379 4173 4193 3379
Size: 794 20 5049
Fragments arranged by size:
5049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2371 2666
Size: 54 1425 50 275 18 270 271 295
Cuts at: 2666 2760 3033 3045 3087 3310 4199 5828 8
Size: 94 273 12 42 223 889 1629 43
Fragments arranged by size:
1629 1425 889 295 275 273 271 270
223 94 54 50 43 42 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2760 3045 3310 1537
Size: 275 948 285 265 4090
Fragments arranged by size:
4090 948 285 275 265
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 2183 3793 1353
Size: 803 27 1610 3423
Fragments arranged by size:
3423 1610 803 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2191 3092 4450 4824 4970 387
Size: 1064 138 602 901 1358 374 146 1280
Fragments arranged by size:
1358 1280 1064 901 602 374 146 138
EcoICRI GAG'CTC
Cuts at: 2649 2649
Size: 5863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2119 2201 2300 2807 2822 3926
Size: 393 412 75 82 99 507 15 1104
Cuts at: 3926 4523 5310 5578 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1104 787 597 507 412 393 268
99 82 75 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2855 2855
Size: 5863
EcoO109I rG'GnC_Cy
Cuts at: 2030 2095 2545 2638 2030
Size: 65 450 93 5255
Fragments arranged by size:
5255 450 93 65
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 5010 1678
Size: 3332 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2591 2623 4504 4536 4578
Size: 32 135 32 1019 32 1881 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 1881 1019 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2720 2928 748
Size: 1972 208 3683
Fragments arranged by size:
3683 1972 208
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2150 4977 5161 1305
Size: 845 2827 184 2007
Fragments arranged by size:
2827 2007 845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 2373 3089 10
Size: 54 1425 343 270 271 716 2784
Fragments arranged by size:
2784 1425 716 343 271 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2585 3821 4180 4339 518
Size: 648 942 477 1236 359 159 2042
Fragments arranged by size:
2042 1236 942 648 477 359 159
MspA1I CmG'CkG
Cuts at: 1606 2139 2377 2704 3097 3279 3956 5104 5349
Size: 533 238 327 393 182 677 1148 245
Cuts at: 5349 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 533 393 327 245 238
182
NaeI GCC'GGC
Cuts at: 3052 3052
Size: 5863
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5311
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NgoMIV G'CCGG_C
Cuts at: 3050 3050
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3045 3310 3045
Size: 265 5598
NspI r_CATG'y
Cuts at: 1132 1229 3085 3221 4766 1132
Size: 97 1856 136 1545 2229
Fragments arranged by size:
2229 1856 1545 136 97
PasI CC'CwG_GG
Cuts at: 1348 2091 1348
Size: 743 5120
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2380 1108
Size: 1272 4591
PfoI T'CCnGG_A
Cuts at: 2010 2784 2010
Size: 774 5089
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2545 2638 2545
Size: 93 5770
PshAI GACnn'nnGTC
Cuts at: 1613 2061 1613
Size: 448 5415
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PspOMI G'GGCC_C
Cuts at: 2094 2094
Size: 5863
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 5831
PstI C_TGCA'G
Cuts at: 830 2343 2694 2859 3215 830
Size: 1513 351 165 356 3478
Fragments arranged by size:
3478 1513 356 351 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2139 2377 2139
Size: 238 5625
RsrII CG'GwC_CG
Cuts at: 3136 3136
Size: 5863
SacI G_AGCT'C
Cuts at: 2651 2651
Size: 5863
SacII CC_GC'GG
Cuts at: 2705 3098 2705
Size: 393 5470
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SanDI GG'GwC_CC
Cuts at: 2638 2638
Size: 5863
SapI GCTCTTCn'nnn_
Cuts at: 2156 2183 2156
Size: 27 5836
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 2345 4111 5823 2345
Size: 1766 1712 2385
Fragments arranged by size:
2385 1766 1712
SfcI C'TryA_G
Cuts at: 826 1045 2339 2690 2855 3211 4346 5027 5218
Size: 219 1294 351 165 356 1135 681 191
Cuts at: 5218 826
Size: 1471
Fragments arranged by size:
1471 1294 1135 681 356 351 219 191
165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 3050 1700
Size: 1350 4513
SmaI CCC'GGG
Cuts at: 1872 2028 3190 1872
Size: 156 1162 4545
Fragments arranged by size:
4545 1162 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3967 4868 5130 820
Size: 197 926 2024 901 262 1553
Fragments arranged by size:
2024 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 2124 2678 1517
Size: 607 554 4702
Fragments arranged by size:
4702 607 554
StyI C'CwwG_G
Cuts at: 513 1065 2415 2490 3302 513
Size: 552 1350 75 812 3074
Fragments arranged by size:
3074 1350 812 552 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3153 3890 3907 4060 4245 4690 325
Size: 1255 1573 737 17 153 185 445 1498
Fragments arranged by size:
1573 1498 1255 737 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 4109 5821
Size: 274 80 33 51 1443 366 1766 1712
Cuts at: 5821 96
Size: 138
Fragments arranged by size:
1766 1712 1443 366 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5823 5855 5823
Size: 32 5831
Tth111I GACn'n_nGTC
Cuts at: 1316 2957 1316
Size: 1641 4222
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2088 2110 2676 3028 2088
Size: 22 566 352 4923
Fragments arranged by size:
4923 566 352 22
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 2026 3188 1870
Size: 156 1162 4545
Fragments arranged by size:
4545 1162 156
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI SexAI SfiI SfoI SrfI SwaI