Restriction map for
pFN21AB6509
(Circular) (Six-base) MAPSORT of: pFN21AB6509.seq Check: 9173 from: 1 to: 5782
pFN21AB6509 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2895 3045 3125 1937
Size: 958 150 80 4594
Fragments arranged by size:
4594 958 150 80
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 2444 2447 2582 3019 3845 5229 5497
Size: 704 108 3 135 437 826 1384 268
Cuts at: 5497 1632
Size: 1917
Fragments arranged by size:
1917 1384 826 704 437 268 135 108
3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2495 1166
Size: 1329 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2467 3525 5097 2467
Size: 1058 1572 3152
Fragments arranged by size:
3152 1572 1058
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
AvrII C'CTAG_G
Cuts at: 2152 2152
Size: 5782
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2838 2871 2838
Size: 33 5749
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 3103 4558 618
Size: 325 217 627 762 554 1455 1842
Fragments arranged by size:
1842 1455 762 627 554 325 217
BanII G_rGCy'C
Cuts at: 1391 2148 2570 3101 1391
Size: 757 422 531 4072
Fragments arranged by size:
4072 757 531 422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2651 928
Size: 971 752 4059
Fragments arranged by size:
4059 971 752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4393 136
Size: 107 122 71 631 605 337 2384 1525
Fragments arranged by size:
2384 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2476 2552 3843 4999 1790
Size: 378 308 76 1291 1156 2573
Fragments arranged by size:
2573 1291 1156 378 308 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2153 2555 3022
Size: 416 53 83 399 24 736 402 467
Cuts at: 3022 3513 4471 442
Size: 491 958 1753
Fragments arranged by size:
1753 958 736 491 467 416 402 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2724 1056
Size: 1668 4114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2391 4442 1239
Size: 1152 2051 2579
Fragments arranged by size:
2579 2051 1152
Bpu10I CC'TnA_GC
Cuts at: 2569 2569
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2637 3907 4772 5070 2637
Size: 1270 865 298 3349
Fragments arranged by size:
3349 1270 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3049 3573 493
Size: 711 1845 524 2702
Fragments arranged by size:
2702 1845 711 524
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5557
Fragments arranged by size:
5557 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5782
BseYI C'CCAG_C
Cuts at: 4985 5481 4985
Size: 496 5286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2570 3843 3928 4999 2570
Size: 1273 85 1071 3353
Fragments arranged by size:
3353 1273 1071 85
BsmI GAATG_Cn'
Cuts at: 2646 3293 3386 2646
Size: 647 93 5042
Fragments arranged by size:
5042 647 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2168 2476 2552 2570 3101 3843
Size: 399 358 20 308 76 18 531 742
Cuts at: 3843 3928 4999 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 531 399 358 308 85
76 20 18
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 3137 844
Size: 1430 863 3489
Fragments arranged by size:
3489 1430 863
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2622 4277 4451 65
Size: 1183 1374 1655 174 1396
Fragments arranged by size:
1655 1396 1374 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2236 3112 3479 3864 3881 5333 5419 5431
Size: 264 876 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 876 385 367 346 264 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2799 3229 4118
Size: 54 1425 50 275 18 969 430 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 969 889 430 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2463 2558 3712 1353
Size: 1110 95 1154 3423
Fragments arranged by size:
3423 1154 1110 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2568 2568
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2391 2444 2447 2582 3019 3845
Size: 393 704 55 53 3 135 437 826
Cuts at: 3845 4442 5229 5497 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 826 787 704 597 437 393 268
135 55 53 3
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 4423 4455 4497
Size: 32 135 32 1205 32 1614 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1614 1205 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 2785 3126 3372 669
Size: 988 281 406 441 341 246 3079
Fragments arranged by size:
3079 988 441 406 341 281 246
HindIII A'AGCT_T
Cuts at: 748 2843 748
Size: 2095 3687
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2905 4896 5080 1305
Size: 1071 529 1991 184 2007
Fragments arranged by size:
2007 1991 1071 529 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2801 10
Size: 54 1425 343 969 2991
Fragments arranged by size:
2991 1425 969 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 2495 2688 3740 4099 4258 518
Size: 648 1315 14 193 1052 359 159 2042
Fragments arranged by size:
2042 1315 1052 648 359 193 159 14
MspA1I CmG'CkG
Cuts at: 1606 2175 2425 2560 3198 3875 5023 5268 1606
Size: 569 250 135 638 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 638 569 250 245 135
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 2720 1052
Size: 1668 4114
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PfoI T'CCnGG_A
Cuts at: 2368 2368
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 3134 830
Size: 2304 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2425 2425
Size: 5782
SacI G_AGCT'C
Cuts at: 2570 2570
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SapI GCTCTTCn'nnn_
Cuts at: 2558 2558
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 3011 4030 5742 3011
Size: 1019 1712 3051
Fragments arranged by size:
3051 1712 1019
SexAI A'CCwGG_T
Cuts at: 2857 2857
Size: 5782
SfcI C'TryA_G
Cuts at: 826 1045 2919 3130 4265 4946 5137 826
Size: 219 1874 211 1135 681 191 1471
Fragments arranged by size:
1874 1471 1135 681 219 211 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2652 3886 4787 5049 820
Size: 197 926 709 1234 901 262 1553
Fragments arranged by size:
1553 1234 926 901 709 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2152 3221 513
Size: 552 1087 1069 3074
Fragments arranged by size:
3074 1087 1069 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3809 3826 3979 4164 4609 325
Size: 1255 2229 17 153 185 445 1498
Fragments arranged by size:
2229 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 3009 4028
Size: 274 80 33 51 1443 903 129 1019
Cuts at: 4028 5740 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1019 903 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2632 2664 5742 5774 2632
Size: 32 3078 32 2640
Fragments arranged by size:
3078 2640 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI
XcmI