Restriction map for
pFN21AB6507
(Circular) (Six-base) MAPSORT of: pFN21AB6507.seq Check: 6802 from: 1 to: 5782
pFN21AB6507 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2241 3125 1937
Size: 304 884 4594
Fragments arranged by size:
4594 884 304
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2954 3845 5229 5497 1632
Size: 1322 891 1384 268 1917
Fragments arranged by size:
1917 1384 1322 891 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2990 4681 5658 1203
Size: 1787 1691 977 1327
Fragments arranged by size:
1787 1691 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 5750
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2419 2882 3107 1870
Size: 73 476 463 225 4545
Fragments arranged by size:
4545 476 463 225 73
BamHI G'GATC_C
Cuts at: 2652 3112 3479 2652
Size: 460 367 4955
Fragments arranged by size:
4955 460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 3103 4558 618
Size: 325 217 627 408 908 1455 1842
Fragments arranged by size:
1842 1455 908 627 408 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 3101 1391
Size: 733 977 4072
Fragments arranged by size:
4072 977 733
BbeI G_GCGC'C
Cuts at: 2199 2199
Size: 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BbvCI CC'TCA_GC
Cuts at: 2699 2699
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2267 2915 4393
Size: 107 122 71 631 605 595 648 1478
Cuts at: 4393 136
Size: 1525
Fragments arranged by size:
1525 1478 648 631 605 595 122 107
71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2559 3843 4999 1790
Size: 769 1284 1156 2573
Fragments arranged by size:
2573 1284 1156 769
BmgBI CAC'GTC
Cuts at: 1318 2356 2539 1318
Size: 1038 183 4561
Fragments arranged by size:
4561 1038 183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2612 2861 4442 1239
Size: 794 579 249 1581 2579
Fragments arranged by size:
2579 1581 794 579 249
Bpu10I CC'TnA_GC
Cuts at: 2036 2699 2036
Size: 663 5119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2279 3047 3907 4772 5070 2108
Size: 171 768 860 865 298 2820
Fragments arranged by size:
2820 865 860 768 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2991 3573 493
Size: 711 1787 582 2702
Fragments arranged by size:
2702 1787 711 582
BsaBI GATnn'nnATC
Cuts at: 2183 5776 2183
Size: 3593 2189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2109 2196 2907 3589
Size: 53 83 186 936 576 87 711 682
Cuts at: 3589 3971 4606 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 711 682 635 576 382 186
87 83 53
BsaWI w'CCGG_w
Cuts at: 2607 2742 2769 4215 4613 4887 5034 5445 2607
Size: 135 27 1446 398 274 147 411 2944
Fragments arranged by size:
2944 1446 411 398 274 147 135 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2447 2477 1178
Size: 30 165 30 1044 30 4483
Fragments arranged by size:
4483 1044 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2765 2794 1186
Size: 1039 540 29 4174
Fragments arranged by size:
4174 1039 540 29
BseYI C'CCAG_C
Cuts at: 2529 2898 4985 5481 2529
Size: 369 2087 496 2830
Fragments arranged by size:
2830 2087 496 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2443 2636 3033 3232 3993 5021
Size: 275 186 442 193 397 199 761 1028
Cuts at: 5021 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 442 397 275 199 193
186
BsiHKAI G_wGCw'C
Cuts at: 2124 2165 3843 3928 4999 2124
Size: 41 1678 85 1071 2907
Fragments arranged by size:
2907 1678 1071 85 41
BsmI GAATG_Cn'
Cuts at: 2438 3293 3386 2438
Size: 855 93 4834
Fragments arranged by size:
4834 855 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2282 2282
Size: 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2165 2559 3101 3843 3928 4999
Size: 399 334 41 394 542 742 85 1071
Cuts at: 4999 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 542 399 394 334 85
41
BspEI T'CCGG_A
Cuts at: 2607 2769 5445 2607
Size: 162 2676 2944
Fragments arranged by size:
2944 2676 162
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2529 2844 3137 844
Size: 1685 315 293 3489
Fragments arranged by size:
3489 1685 315 293
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 2641 3669 1394
Size: 429 331 487 1028 3507
Fragments arranged by size:
3507 1028 487 429 331
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 2564 4426 4613 1700
Size: 110 388 366 1862 187 2869
Fragments arranged by size:
2869 1862 388 366 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 794
Size: 1745 4037
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 2365 1184
Size: 1181 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2298 2652 2850 3112 3479 3864 3881 5333
Size: 326 354 198 262 367 385 17 1452
Cuts at: 5333 5419 5431 5777 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 354 346 326 262
198 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2191 2283 513
Size: 552 1126 92 4012
Fragments arranged by size:
4012 1126 552 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2055 2488 487
Size: 683 56 829 433 3781
Fragments arranged by size:
3781 829 683 433 56
BtsI GCAGTG_nn'
Cuts at: 2656 3298 4092 4112 2656
Size: 642 794 20 4326
Fragments arranged by size:
4326 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DraIII CAC_nnn'GTG
Cuts at: 2869 2869
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 4789 809
Size: 507 698 2775 1802
Fragments arranged by size:
2775 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3030 3229 4118
Size: 54 1425 50 275 18 1200 199 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1200 889 275 199 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3030 3229 1537
Size: 275 1218 199 4090
Fragments arranged by size:
4090 1218 275 199
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2822 3712 1353
Size: 800 669 890 3423
Fragments arranged by size:
3423 890 800 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2122
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2612 2861 2954 3845 4442 5229
Size: 393 401 579 249 93 891 597 787
Cuts at: 5229 5497 1239
Size: 268 1524
Fragments arranged by size:
1524 891 787 597 579 401 393 268
249 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2840 2411
Size: 429 5353
EcoO109I rG'GnC_Cy
Cuts at: 2416 2833 2416
Size: 417 5365
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 5782
FspI TGC'GCA
Cuts at: 2183 4288 2183
Size: 2105 3677
FspAI rTGC'GCAy
Cuts at: 2183 2183
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2199 4929 1678
Size: 521 2730 2531
Fragments arranged by size:
2730 2531 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 3126 3372 669
Size: 988 281 559 629 246 3079
Fragments arranged by size:
3079 988 629 559 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KasI G'GCGC_C
Cuts at: 2195 2195
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 2616 2709 4896 5080 1305
Size: 1185 126 93 2187 184 2007
Fragments arranged by size:
2187 2007 1185 184 126 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 3740 4099 4258 518
Size: 648 1095 1479 359 159 2042
Fragments arranged by size:
2042 1479 1095 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2632 2902 3198 3875 5023 5268 1606
Size: 483 543 270 296 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 543 483 296 270 245
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 5782
NarI GG'CG_CC
Cuts at: 2196 2196
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 2283 513
Size: 552 1218 4012
Fragments arranged by size:
4012 1218 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2719 1108
Size: 1611 4171
PfoI T'CCnGG_A
Cuts at: 2343 2427 2343
Size: 84 5698
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2991 2991
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2416 2833 2416
Size: 417 5365
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 3054 3134 830
Size: 2224 80 3478
Fragments arranged by size:
3478 2224 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2089 2089
Size: 5782
SacI G_AGCT'C
Cuts at: 2124 2124
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SapI GCTCTTCn'nnn_
Cuts at: 2153 2153
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 2480 4030 5742 2480
Size: 1550 1712 2520
Fragments arranged by size:
2520 1712 1550
SexAI A'CCwGG_T
Cuts at: 2865 3075 2865
Size: 210 5572
SfcI C'TryA_G
Cuts at: 826 1045 3050 3130 4265 4946 5137 826
Size: 219 2005 80 1135 681 191 1471
Fragments arranged by size:
2005 1471 1135 681 219 191 80
SfoI GGC'GCC
Cuts at: 2197 2197
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2421 3109 1872
Size: 549 688 4545
Fragments arranged by size:
4545 688 549
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2123 2294 3062 3886 4787 5049
Size: 197 926 180 171 768 824 901 262
Cuts at: 5049 820
Size: 1553
Fragments arranged by size:
1553 926 901 824 768 262 197 180
171
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2283 2511 3221 513
Size: 552 1218 228 710 3074
Fragments arranged by size:
3074 1218 710 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 2543 3809 3826 3979 4164 4609
Size: 1255 924 39 1266 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1498 1266 1255 924 445 185 153 39
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 2856 3069
Size: 274 80 33 51 1443 501 378 213
Cuts at: 3069 4028 5740 96
Size: 959 1712 138
Fragments arranged by size:
1712 1443 959 501 378 274 213 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 2714 1316
Size: 1398 4384
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 2419 3107 1870
Size: 549 688 4545
Fragments arranged by size:
4545 688 549
XmnI GAAnn'nnTTC
Cuts at: 2252 2939 3911 2252
Size: 687 972 4123
Fragments arranged by size:
4123 972 687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoRV FseI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI
SwaI XcmI