Restriction map for
pFN21AB6504
(Circular) (Six-base) MAPSORT of: pFN21AB6504.seq Check: 10 from: 1 to: 5851
pFN21AB6504 5851 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3010 3172 3010
Size: 162 5689
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 3914 5298 5566 1632
Size: 1076 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 1076 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2312 3078 1184
Size: 1128 766 3957
Fragments arranged by size:
3957 1128 766
AlwNI CAG_nnn'CTG
Cuts at: 3594 5166 3594
Size: 1572 4279
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3160 3386 3538 5647 1493
Size: 1667 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2323 2332 2954 3176 1870
Size: 73 380 9 622 222 4545
Fragments arranged by size:
4545 622 380 222 73 9
BamHI G'GATC_C
Cuts at: 2269 2407 2778 3181 3548 2269
Size: 138 371 403 367 4572
Fragments arranged by size:
4572 403 371 367 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 2727 3010 3172 4627
Size: 325 217 627 382 558 283 162 1455
Cuts at: 4627 618
Size: 1842
Fragments arranged by size:
1842 1455 627 558 382 325 283 217
162
BanII G_rGCy'C
Cuts at: 1391 2141 2796 2824 2964 3150 3170 1391
Size: 750 655 28 140 186 20 4072
Fragments arranged by size:
4072 750 655 186 140 28 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2985 928
Size: 971 1086 3794
Fragments arranged by size:
3794 1086 971
BbvCI CC'TCA_GC
Cuts at: 2879 2879
Size: 5851
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4042 4076 1903
Size: 34 50 34 2021 34 3678
Fragments arranged by size:
3678 2021 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4462 136
Size: 107 122 71 631 605 2790 1525
Fragments arranged by size:
2790 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 2618 3268 2618
Size: 650 5201
Bme1580I G_kGCm'C
Cuts at: 1790 2574 2581 3912 5068 1790
Size: 784 7 1331 1156 2573
Fragments arranged by size:
2573 1331 1156 784 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 2381 2609 2633 4511 1239
Size: 1066 76 228 24 1878 2579
Fragments arranged by size:
2579 1878 1066 228 76 24
Bpu10I CC'TnA_GC
Cuts at: 2879 2879
Size: 5851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3976 4841 5139 3976
Size: 865 298 4688
Fragments arranged by size:
4688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4514 882
Size: 617 3015 2219
Fragments arranged by size:
3015 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2398 3642 493
Size: 711 1194 1244 2702
Fragments arranged by size:
2702 1244 1194 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4040 4675 275
Size: 53 83 186 936 2125 382 635 1451
Fragments arranged by size:
2125 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3003 3033 1178
Size: 30 165 30 1600 30 3996
Fragments arranged by size:
3996 1600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2896 1186
Size: 1012 698 4141
Fragments arranged by size:
4141 1012 698
BseYI C'CCAG_C
Cuts at: 2041 2194 2736 5054 5550 2041
Size: 153 542 2318 496 2342
Fragments arranged by size:
2342 2318 542 496 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2885 1163
Size: 573 1149 4129
Fragments arranged by size:
4129 1149 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2291 2796 3021 3912 3997 5068 2291
Size: 505 225 891 85 1071 3074
Fragments arranged by size:
3074 1071 891 505 225 85
BsmI GAATG_Cn'
Cuts at: 3114 3362 3455 3114
Size: 248 93 5510
Fragments arranged by size:
5510 248 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 2285
Size: 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2291 2574 2581 2796 2824 2964
Size: 399 351 150 283 7 215 28 140
Cuts at: 2964 3021 3150 3170 3912 3997 5068 1391
Size: 57 129 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 283 215 150
140 129 85 57 28 20 7
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2378 2922 3206 844
Size: 1534 544 284 3489
Fragments arranged by size:
3489 1534 544 284
BsrBI CCG'CTC
Cuts at: 1394 1823 2163 3738 1394
Size: 429 340 1575 3507
Fragments arranged by size:
3507 1575 429 340
BsrDI GCAATG_nn'
Cuts at: 65 1248 2458 4346 4520 65
Size: 1183 1210 1888 174 1396
Fragments arranged by size:
1888 1396 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4495 4682 1700
Size: 110 2685 187 2869
Fragments arranged by size:
2869 2685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 2647 1184
Size: 1463 4388
BstYI r'GATC_y
Cuts at: 1972 2269 2407 2778 3181 3548 3933 3950 5402
Size: 297 138 371 403 367 385 17 1452
Cuts at: 5402 5488 5500 5846 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 403 385 371 367 346 297
138 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2020 2096 2133 2556 3093 513
Size: 552 955 76 37 423 537 3271
Fragments arranged by size:
3271 955 552 537 423 76 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 3367 4161 4181 3367
Size: 794 20 5037
Fragments arranged by size:
5037 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3074 4858 809
Size: 507 1758 1784 1802
Fragments arranged by size:
1802 1784 1758 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2460 3298
Size: 54 1425 50 275 18 300 330 838
Cuts at: 3298 4187 5816 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 838 330 300 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 2669 2673 3781 1353
Size: 1175 141 4 1108 3423
Fragments arranged by size:
3423 1175 1108 141 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4438 4812 4958 387
Size: 1064 138 2849 374 146 1280
Fragments arranged by size:
2849 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2794 2794
Size: 5851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 2381 2609 2633 2708 3914 4511
Size: 393 673 76 228 24 75 1206 597
Cuts at: 4511 5298 5566 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1206 787 673 597 393 268 228
76 75 24
EcoO109I rG'GnC_Cy
Cuts at: 2212 2699 2212
Size: 487 5364
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 2086 2129 2687 4998 1678
Size: 408 43 558 2311 2531
Fragments arranged by size:
2531 2311 558 408 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2278 2310 4492 4524 4566
Size: 32 135 32 706 32 2182 32 42
Cuts at: 4566 4598 1373
Size: 32 2626
Fragments arranged by size:
2626 2182 706 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3014 3176 3014
Size: 162 5689
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2974 4965 5149 1305
Size: 1669 1991 184 2007
Fragments arranged by size:
2007 1991 1669 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 10
Size: 54 1425 343 630 3399
Fragments arranged by size:
3399 1425 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3809 4168 4327 518
Size: 648 2643 359 159 2042
Fragments arranged by size:
2643 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2098 2194 2865 3267 3944 5092 5337
Size: 416 76 96 671 402 677 1148 245
Cuts at: 5337 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 671 416 402 245 96
76
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5299
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 3209 4754 1132
Size: 97 1980 1545 2229
Fragments arranged by size:
2229 1980 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2431 1348
Size: 1083 4768
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2743 1108
Size: 1635 4216
PfoI T'CCnGG_A
Cuts at: 2358 2358
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PmlI CAC'GTG
Cuts at: 2398 2398
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2040 2072 3920 3952 5433 5465 2040
Size: 32 1848 32 1481 32 2426
Fragments arranged by size:
2426 1848 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2699 2212
Size: 487 5364
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PstI C_TGCA'G
Cuts at: 830 2922 3203 830
Size: 2092 281 3478
Fragments arranged by size:
3478 2092 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
RsrII CG'GwC_CG
Cuts at: 2860 2860
Size: 5851
SacI G_AGCT'C
Cuts at: 2796 2796
Size: 5851
SacII CC_GC'GG
Cuts at: 2023 2099 2023
Size: 76 5775
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SanDI GG'GwC_CC
Cuts at: 2212 2699 2212
Size: 487 5364
SapI GCTCTTCn'nnn_
Cuts at: 2528 2669 2528
Size: 141 5710
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 2839 2918 3199 4334 5015 5206 826
Size: 219 1794 79 281 1135 681 191 1471
Fragments arranged by size:
1794 1471 1135 681 281 219 191 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 2325 2334 2956 3178 1872
Size: 453 9 622 222 4545
Fragments arranged by size:
4545 622 453 222 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3955 4856 5118 820
Size: 197 926 2012 901 262 1553
Fragments arranged by size:
2012 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2474 2703 3290 513
Size: 552 1409 229 587 3074
Fragments arranged by size:
3074 1409 587 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3102 3106 3878 3895 4048 4233 4678
Size: 1255 1522 4 772 17 153 185 445
Cuts at: 4678 325
Size: 1498
Fragments arranged by size:
1522 1498 1255 772 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2943 4097 5809
Size: 274 80 33 51 1443 966 1154 1712
Cuts at: 5809 96
Size: 138
Fragments arranged by size:
1712 1443 1154 966 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5851
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2280 2280
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 2323 2332 2954 3176 1870
Size: 453 9 622 222 4545
Fragments arranged by size:
4545 622 453 222 9
XmnI GAAnn'nnTTC
Cuts at: 2522 3980 2522
Size: 1458 4393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI PvuII SexAI
SfiI SfoI SrfI SwaI