Restriction map for
pFN21AB6502
(Circular) (Six-base) MAPSORT of: pFN21AB6502.seq Check: 6 from: 1 to: 5848
pFN21AB6502 5848 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3120 3191 1937
Size: 1183 71 4594
Fragments arranged by size:
4594 1183 71
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2395 3911 5295 5563 1632
Size: 763 1516 1384 268 1917
Fragments arranged by size:
1917 1516 1384 763 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 2682 4747 5724 1203
Size: 1479 2065 977 1327
Fragments arranged by size:
2065 1479 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 2545 4577 2545
Size: 2032 3816
AleI CACnn'nnGTG
Cuts at: 1166 2288 1166
Size: 1122 4726
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2207 1184
Size: 1023 4825
AlwNI CAG_nnn'CTG
Cuts at: 2316 2909 3025 3088 3591 5163 2316
Size: 593 116 63 503 1572 3001
Fragments arranged by size:
3001 1572 593 503 116 63
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2693 3157 3383 3535 5644 1493
Size: 1200 464 226 152 2109 1697
Fragments arranged by size:
2109 1697 1200 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 2179 2348 2504 2850 3173 1870
Size: 73 236 169 156 346 323 4545
Fragments arranged by size:
4545 346 323 236 169 156 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2196 2276 3169 4624 618
Size: 325 217 627 409 80 893 1455 1842
Fragments arranged by size:
1842 1455 893 627 409 325 217 80
BanII G_rGCy'C
Cuts at: 1391 2505 3167 1391
Size: 1114 662 4072
Fragments arranged by size:
4072 1114 662
BbeI G_GCGC'C
Cuts at: 2200 2200
Size: 5848
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2565 2599 4039 4073 1903
Size: 34 628 34 1440 34 3678
Fragments arranged by size:
3678 1440 628 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2631 5843 2631
Size: 3212 2636
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2031 3909 5065 1790
Size: 241 1878 1156 2573
Fragments arranged by size:
2573 1878 1156 241
BmgBI CAC'GTC
Cuts at: 1318 2683 1318
Size: 1365 4483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2618 2806 3579
Size: 416 53 83 399 24 1201 188 773
Cuts at: 3579 4537 442
Size: 958 1753
Fragments arranged by size:
1753 1201 958 773 416 399 188 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2815 4508 1239
Size: 866 710 1693 2579
Fragments arranged by size:
2579 1693 866 710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2354 3973 4838 5136 2345
Size: 9 1619 865 298 3057
Fragments arranged by size:
3057 1619 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2441 2556 4511 882
Size: 617 942 115 1955 2219
Fragments arranged by size:
2219 1955 942 617 115
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2197 3655 4037 4672
Size: 53 83 186 936 664 1458 382 635
Cuts at: 4672 275
Size: 1451
Fragments arranged by size:
1458 1451 936 664 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5848
BseYI C'CCAG_C
Cuts at: 2089 2497 3043 5051 5547 2089
Size: 408 546 2008 496 2390
Fragments arranged by size:
2390 2008 546 496 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2126 3298 4059 5087 1540
Size: 275 186 125 1172 761 1028 2301
Fragments arranged by size:
2301 1172 1028 761 275 186 125
BsiHKAI G_wGCw'C
Cuts at: 2295 3909 3994 5065 2295
Size: 1614 85 1071 3078
Fragments arranged by size:
3078 1614 1071 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2295 2505 3167 3909 3994 5065
Size: 399 241 264 210 662 742 85 1071
Cuts at: 5065 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 662 399 264 241 210
85
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 3203 844
Size: 1379 980 3489
Fragments arranged by size:
3489 1379 980
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 2901 3735 1394
Size: 429 278 800 834 3507
Fragments arranged by size:
3507 834 800 429 278
BsrDI GCAATG_nn'
Cuts at: 65 1248 2440 2878 4343 4517 65
Size: 1183 1192 438 1465 174 1396
Fragments arranged by size:
1465 1396 1192 1183 438 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2218 4492 4679 1700
Size: 110 311 97 2274 187 2869
Fragments arranged by size:
2869 2274 311 187 110 97
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 2224 2226 1925
Size: 299 2 5547
Fragments arranged by size:
5547 299 2
BssSI C'ACGA_G
Cuts at: 2464 3908 4920 2464
Size: 1444 1012 3392
Fragments arranged by size:
3392 1444 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 2210 1184
Size: 1026 4822
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 2677 2170
Size: 507 5341
BstYI r'GATC_y
Cuts at: 1972 2631 3178 3545 3930 3947 5399 5485 5497
Size: 659 547 367 385 17 1452 86 12
Cuts at: 5497 5843 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 659 547 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3121 3121
Size: 5848
Bsu36I CC'TnA_GG
Cuts at: 2823 2823
Size: 5848
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3364 4158 4178 3364
Size: 794 20 5034
Fragments arranged by size:
5034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2845 2880 529
Size: 35 2281 35 3497
Fragments arranged by size:
3497 2281 35 35
DraI TTT'AAA
Cuts at: 2764 3152 2764
Size: 388 5460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2123 2160 2172
Size: 54 1425 50 275 18 293 37 12
Cuts at: 2172 3295 4184 5813 8
Size: 1123 889 1629 43
Fragments arranged by size:
1629 1425 1123 889 293 275 54 50
43 37 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2123 3295 1537
Size: 275 311 1172 4090
Fragments arranged by size:
4090 1172 311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2536 3778 1353
Size: 884 299 1242 3423
Fragments arranged by size:
3423 1242 884 299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2395 2815 3911 4508 5295 5563
Size: 393 473 290 420 1096 597 787 268
Cuts at: 5563 1239
Size: 1524
Fragments arranged by size:
1524 1096 787 597 473 420 393 290
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2204
Size: 5848
EcoO109I rG'GnC_Cy
Cuts at: 2509 2509
Size: 5848
EcoRI G'AATT_C
Cuts at: 2693 3157 3535 2693
Size: 464 378 5006
Fragments arranged by size:
5006 464 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 5848
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2200 2272 4995 1678
Size: 522 72 2723 2531
Fragments arranged by size:
2723 2531 522 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2519 2531 2551 2563 4489
Size: 32 135 32 947 12 20 12 1926
Cuts at: 4489 4521 4563 4595 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1926 947 135 42 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KasI G'GCGC_C
Cuts at: 2196 2196
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 2628 2667 3012 3132 4962 5146 1305
Size: 714 609 39 345 120 1830 184 2007
Fragments arranged by size:
2007 1830 714 609 345 184 120 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 10
Size: 54 1425 343 342 3684
Fragments arranged by size:
3684 1425 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2288 3806 4165 4324 518
Size: 648 1002 120 1518 359 159 2042
Fragments arranged by size:
2042 1518 1002 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2083 2599 3022 3088 3264 3941 5089 5334
Size: 477 516 423 66 176 677 1148 245
Cuts at: 5334 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 516 477 423 245 176
66
NaeI GCC'GGC
Cuts at: 2123 2123
Size: 5848
NarI GG'CG_CC
Cuts at: 2197 2197
Size: 5848
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5296
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NgoMIV G'CCGG_C
Cuts at: 2121 2121
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 3206 4751 1132
Size: 97 1977 1545 2229
Fragments arranged by size:
2229 1977 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PstI C_TGCA'G
Cuts at: 830 2205 3200 830
Size: 1375 995 3478
Fragments arranged by size:
3478 1375 995
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2083 3022 3088 2083
Size: 939 66 4843
Fragments arranged by size:
4843 939 66
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2237
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SexAI A'CCwGG_T
Cuts at: 3123 3123
Size: 5848
SfcI C'TryA_G
Cuts at: 826 1045 2201 3196 4331 5012 5203 826
Size: 219 1156 995 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 995 681 219 191
SfoI GGC'GCC
Cuts at: 2198 2198
Size: 5848
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 2181 2852 3175 1872
Size: 309 671 323 4545
Fragments arranged by size:
4545 671 323 309
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 2369 3952 4853 5115 820
Size: 197 926 417 9 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 417 262 197 9
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 3287 513
Size: 552 2222 3074
Fragments arranged by size:
3074 2222 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2574 3875 3892 4045 4230 4675 325
Size: 1255 994 1301 17 153 185 445 1498
Fragments arranged by size:
1498 1301 1255 994 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 4094 5806
Size: 274 80 33 51 1443 729 1388 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1712 1443 1388 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 2969 1316
Size: 1653 4195
XbaI T'CTAG_A
Cuts at: 2455 3184 2455
Size: 729 5119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 2179 2850 3173 1870
Size: 309 671 323 4545
Fragments arranged by size:
4545 671 323 309
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI