Restriction map for
pFN21AB6496
(Circular) (Six-base) MAPSORT of: pFN21AB6496.seq Check: 9925 from: 1 to: 5779
pFN21AB6496 5779 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3057 3122 1937
Size: 1120 65 4594
Fragments arranged by size:
4594 1120 65
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3842 5226 5494 1632
Size: 2210 1384 268 1917
Fragments arranged by size:
2210 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 2786 1166
Size: 1620 4159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 2020 2212 3031 3522 5094 2020
Size: 192 819 491 1572 2705
Fragments arranged by size:
2705 1572 819 491 192
ApaI G_GGCC'C
Cuts at: 2549 2549
Size: 5779
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 2201 2449 3088 3314 3466 5575 1493
Size: 708 248 639 226 152 2109 1697
Fragments arranged by size:
2109 1697 708 639 248 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2868 3104 1870
Size: 73 925 236 4545
Fragments arranged by size:
4545 925 236 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 3100 4555 618
Size: 325 217 627 311 1002 1455 1842
Fragments arranged by size:
1842 1455 1002 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2306 2549 2922 2954 3098 1391
Size: 915 243 373 32 144 4072
Fragments arranged by size:
4072 915 373 243 144 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4390 136
Size: 107 122 71 631 605 2718 1525
Fragments arranged by size:
2718 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2088 2413 5774 2088
Size: 325 3361 2093
Fragments arranged by size:
3361 2093 325
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2549 3840 4996 1790
Size: 759 1291 1156 2573
Fragments arranged by size:
2573 1291 1156 759
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2733 4439 1239
Size: 1494 1706 2579
Fragments arranged by size:
2579 1706 1494
Bpu10I CC'TnA_GC
Cuts at: 2021 2021
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2698 3570 493
Size: 711 1494 872 2702
Fragments arranged by size:
2702 1494 872 711
BsaBI GATnn'nnATC
Cuts at: 2295 5773 2295
Size: 3478 2301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 3968 4603 275
Size: 53 83 186 936 2053 382 635 1451
Fragments arranged by size:
2053 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2169 4212 4610 4884 5031 5442 2169
Size: 2043 398 274 147 411 2506
Fragments arranged by size:
2506 2043 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2781 1186
Size: 1595 4184
BseYI C'CCAG_C
Cuts at: 2538 2542 4982 5478 2538
Size: 4 2440 496 2839
Fragments arranged by size:
2839 2440 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3068 1163
Size: 573 1332 3874
Fragments arranged by size:
3874 1332 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 3229 3990 5018 1540
Size: 275 186 169 1059 761 1028 2301
Fragments arranged by size:
2301 1059 1028 761 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 2027 2306 3840 3925 4996 2027
Size: 279 1534 85 1071 2810
Fragments arranged by size:
2810 1534 1071 279 85
BsmI GAATG_Cn'
Cuts at: 2594 3290 3383 2594
Size: 696 93 4990
Fragments arranged by size:
4990 696 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2649 2649
Size: 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2306 2549 2922 2954 3098 3840
Size: 399 237 279 243 373 32 144 742
Cuts at: 3840 3925 4996 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 279 243 237
144 85 32
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 844
Size: 2290 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4423 4610 1700
Size: 110 2613 187 2869
Fragments arranged by size:
2869 2613 187 110
BsrGI T'GTAC_A
Cuts at: 96 2778 96
Size: 2682 3097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2031 2527 794
Size: 1237 496 4046
Fragments arranged by size:
4046 1237 496
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2542 2542
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2088 2209 2413 3109 3476 3861 3878 5330
Size: 116 121 204 696 367 385 17 1452
Cuts at: 5330 5416 5428 5774 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 696 385 367 346 204 121
116 86 17 12
BstZ17I GTA'TAC
Cuts at: 3058 3058
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2004 2308 513
Size: 552 939 304 3984
Fragments arranged by size:
3984 939 552 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3226 4115 5744
Size: 54 1425 50 275 18 1396 889 1629
Cuts at: 5744 8
Size: 43
Fragments arranged by size:
1629 1425 1396 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3709 1353
Size: 2356 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2304 2304
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2733 3842 4439 5226 5494 1239
Size: 393 1101 1109 597 787 268 1524
Fragments arranged by size:
1524 1109 1101 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2546 2546
Size: 5779
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2557 4926 1678
Size: 879 2369 2531
Fragments arranged by size:
2531 2369 879
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 3003 3035 4420
Size: 32 135 32 647 32 752 32 1385
Cuts at: 4420 4452 4494 4526 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1385 752 647 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3123 3369 669
Size: 988 281 1059 126 246 3079
Fragments arranged by size:
3079 1059 988 281 246 126
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 2997 3369 2997
Size: 372 5407
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 2512 3378 2512
Size: 866 4913
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2700 2786 2844 3737 4096 4255 518
Size: 648 1534 86 58 893 359 159 2042
Fragments arranged by size:
2042 1534 893 648 359 159 86 58
MspA1I CmG'CkG
Cuts at: 1606 2542 3195 3872 5020 5265 1606
Size: 936 653 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 677 653 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2308 513
Size: 552 939 304 3984
Fragments arranged by size:
3984 939 552 304
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 3137 4682 1132
Size: 97 1908 1545 2229
Fragments arranged by size:
2229 1908 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2549 1348
Size: 1201 4578
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 2575 1108
Size: 1104 363 4312
Fragments arranged by size:
4312 1104 363
PfoI T'CCnGG_A
Cuts at: 2205 2205
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PmlI CAC'GTG
Cuts at: 2698 2698
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2545 2545
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2157 3131 830
Size: 1327 974 3478
Fragments arranged by size:
3478 1327 974
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2542 2542
Size: 5779
SacI G_AGCT'C
Cuts at: 2306 2306
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2137 2153 3127 4262 4943 5134 826
Size: 219 1092 16 974 1135 681 191 1471
Fragments arranged by size:
1471 1135 1092 974 681 219 191 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 2870 3106 1872
Size: 998 236 4545
Fragments arranged by size:
4545 998 236
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 2605 152
Size: 2453 3326
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 2386 2754 3703 5
Size: 47 2334 368 949 2081
Fragments arranged by size:
2334 2081 949 368 47
StuI AGG'CCT
Cuts at: 1517 2135 1517
Size: 618 5161
StyI C'CwwG_G
Cuts at: 513 1065 2004 2308 3218 513
Size: 552 939 304 910 3074
Fragments arranged by size:
3074 939 910 552 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2552 2763 3806 3823 3976 4161
Size: 1255 624 348 211 1043 17 153 185
Cuts at: 4161 4606 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1043 624 445 348 211 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 4025 5737
Size: 274 80 33 51 1443 801 1247 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1712 1443 1247 801 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 5739 5771 2439
Size: 32 3268 32 2447
Fragments arranged by size:
3268 2447 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 2416 3115 2416
Size: 699 5080
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 2868 3104 1870
Size: 998 236 4545
Fragments arranged by size:
4545 998 236
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI