Restriction map for
pFN21AB6494
(Circular) (Six-base) MAPSORT of: pFN21AB6494.seq Check: 1164 from: 1 to: 5515
pFN21AB6494 5515 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2691 3578 4962 5230 1632
Size: 1059 887 1384 268 1917
Fragments arranged by size:
1917 1384 1059 887 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 2712 4414 5391 1203
Size: 1509 1702 977 1327
Fragments arranged by size:
1702 1509 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 2231 1166
Size: 1065 4450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2644 2824 3050 3202 5311 1493
Size: 1151 180 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 180 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2127 2840 1870
Size: 73 131 53 713 4545
Fragments arranged by size:
4545 713 131 73 53
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 2134 2834 1391
Size: 737 6 700 4072
Fragments arranged by size:
4072 737 700 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4544
BbvCI CC'TCA_GC
Cuts at: 2533 2533
Size: 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2731 3407 4617 2731
Size: 676 1210 3629
Fragments arranged by size:
3629 1210 676
BclI T'GATC_A
Cuts at: 1548 2490 1548
Size: 942 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 136
Size: 107 122 71 631 605 2454 1525
Fragments arranged by size:
2454 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2402 5510 2402
Size: 3108 2407
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2766 3576 4732 1790
Size: 358 618 810 1156 2573
Fragments arranged by size:
2573 1156 810 618 358
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 2471 4175 1239
Size: 1113 119 1704 2579
Fragments arranged by size:
2579 1704 1113 119
Bpu10I CC'TnA_GC
Cuts at: 2533 2533
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2088 2678 3640 4505 4803 2088
Size: 590 962 865 298 2800
Fragments arranged by size:
2800 962 865 590 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3322 3704 4339 275
Size: 53 83 186 936 1789 382 635 1451
Fragments arranged by size:
1789 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5290
Fragments arranged by size:
5290 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 1186
Size: 843 4672
BseYI C'CCAG_C
Cuts at: 2133 4718 5214 2133
Size: 2585 496 2434
Fragments arranged by size:
2585 2434 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2286 1163
Size: 573 550 4392
Fragments arranged by size:
4392 573 550
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2548 3576 3661 4732 2548
Size: 1028 85 1071 3331
Fragments arranged by size:
3331 1071 1028 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2134 2148 2548 2766 2834 3576
Size: 399 338 6 14 400 218 68 742
Cuts at: 3576 3661 4732 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 338 218 85
68 14 6
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2761 4010 4184 65
Size: 1183 1513 1249 174 1396
Fragments arranged by size:
1513 1396 1249 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 4159 4346 1700
Size: 110 244 2105 187 2869
Fragments arranged by size:
2869 2105 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2143 794
Size: 1349 4166
BstBI TT'CG_AA
Cuts at: 2022 4319 2022
Size: 2297 3218
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2402 2689 2845 3212 3597 3614 5066 5152
Size: 430 287 156 367 385 17 1452 86
Cuts at: 5152 5164 5510 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 430 385 367 346 287 156
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2307 513
Size: 552 1242 3721
Fragments arranged by size:
3721 1242 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2353 3031 3825 3845 2353
Size: 678 794 20 4023
Fragments arranged by size:
4023 794 678 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2164 2199 529
Size: 35 1600 35 3845
Fragments arranged by size:
3845 1600 35 35
DraI TTT'AAA
Cuts at: 2797 2819 2797
Size: 22 5493
DraIII CAC_nnn'GTG
Cuts at: 2143 2143
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480
Size: 54 1425 50 275 18 1132 889 1629
Cuts at: 5480 8
Size: 43
Fragments arranged by size:
1629 1425 1132 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 1353
Size: 2092 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2471 2691 3578 4175 4962 5230
Size: 393 720 119 220 887 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 887 787 720 597 393 268 220
119
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 4662 1678
Size: 2984 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2535 2567 4156 4188 4230
Size: 32 135 32 963 32 1589 32 42
Cuts at: 4230 4262 1373
Size: 32 2626
Fragments arranged by size:
2626 1589 963 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2329 2859 3105 669
Size: 988 281 391 530 246 3079
Fragments arranged by size:
3079 988 530 391 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2288 4629 4813 1305
Size: 983 2341 184 2007
Fragments arranged by size:
2341 2007 983 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 3473 3832 3991 518
Size: 648 1065 1242 359 159 2042
Fragments arranged by size:
2042 1242 1065 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2300 2704 2931 3608 4756 5001 1606
Size: 558 136 404 227 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 404 245 227 136
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 2307 513
Size: 552 1242 3721
Fragments arranged by size:
3721 1242 552
NdeI CA'TA_TG
Cuts at: 387 2377 387
Size: 1990 3525
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2252 2716 2873 4418 1132
Size: 97 1023 464 157 1545 2229
Fragments arranged by size:
2229 1545 1023 464 157 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 2712 4414 2712
Size: 1702 3813
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2612 1108
Size: 1504 4011
PfoI T'CCnGG_A
Cuts at: 2560 2560
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2867 830
Size: 2037 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2164 2300 2704 2164
Size: 136 404 4975
Fragments arranged by size:
4975 404 136
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SexAI A'CCwGG_T
Cuts at: 2634 2634
Size: 5515
SfcI C'TryA_G
Cuts at: 826 1045 2863 3998 4679 4870 826
Size: 219 1818 1135 681 191 1471
Fragments arranged by size:
1818 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2076 2842 1872
Size: 204 766 4545
Fragments arranged by size:
4545 766 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2103 2693 3619 4520 4782 820
Size: 197 926 160 590 926 901 262 1553
Fragments arranged by size:
1553 926 926 901 590 262 197 160
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2307 2954 513
Size: 552 1242 647 3074
Fragments arranged by size:
3074 1242 647 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2320 3542 3559 3712 3897 4342 325
Size: 1255 740 1222 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1222 740 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2150 3761 5473
Size: 274 80 33 51 1443 173 1611 1712
Cuts at: 5473 96
Size: 138
Fragments arranged by size:
1712 1611 1443 274 173 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2347 2379 2572 2604 5475 5507 2347
Size: 32 193 32 2871 32 2355
Fragments arranged by size:
2871 2355 193 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2479 2479
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2074 2840 1870
Size: 204 766 4545
Fragments arranged by size:
4545 766 204
XmnI GAAnn'nnTTC
Cuts at: 2465 2597 3644 2465
Size: 132 1047 4336
Fragments arranged by size:
4336 1047 132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI