Restriction map for
pFN21AB6492
(Circular) (Six-base) MAPSORT of: pFN21AB6492.seq Check: 8025 from: 1 to: 5776
pFN21AB6492 5776 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2389 2389
Size: 5776
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2696 3839 5223 5491 1632
Size: 641 423 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1143 641 423 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2669 1184
Size: 1485 4291
AlwNI CAG_nnn'CTG
Cuts at: 2209 2707 3037 3519 5091 2209
Size: 498 330 482 1572 2894
Fragments arranged by size:
2894 1572 498 482 330
ApaI G_GGCC'C
Cuts at: 2079 2079
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2492 3085 3311 3463 5572 1493
Size: 999 593 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 593 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 3101 1870
Size: 73 129 1029 4545
Fragments arranged by size:
4545 1029 129 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2423 3097 4552 618
Size: 325 217 627 221 415 674 1455 1842
Fragments arranged by size:
1842 1455 674 627 415 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2043 2079 2241 2769 2847 3061 3095 1391
Size: 652 36 162 528 78 214 34 4072
Fragments arranged by size:
4072 652 528 214 162 78 36 34
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 5776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2439 2486 928
Size: 971 540 47 4218
Fragments arranged by size:
4218 971 540 47
BbvCI CC'TCA_GC
Cuts at: 2918 2918
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 2736 4387
Size: 107 122 71 631 605 428 636 1651
Cuts at: 4387 136
Size: 1525
Fragments arranged by size:
1651 1525 636 631 605 428 122 107
71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2079 3837 4993 1790
Size: 289 1758 1156 2573
Fragments arranged by size:
2573 1758 1156 289
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2594 2846 2918 3005 3037 4436 1239
Size: 1355 252 72 87 32 1399 2579
Fragments arranged by size:
2579 1399 1355 252 87 72 32
Bpu10I CC'TnA_GC
Cuts at: 2582 2630 2918 2582
Size: 48 288 5440
Fragments arranged by size:
5440 288 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3901 4766 5064 2831
Size: 1070 865 298 3543
Fragments arranged by size:
3543 1070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2570 4439 882
Size: 617 1071 1869 2219
Fragments arranged by size:
2219 1869 1071 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3583 3965 4600
Size: 53 83 186 936 476 1574 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1574 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2153 4209 4607 4881 5028 5439 2153
Size: 2056 398 274 147 411 2490
Fragments arranged by size:
2490 2056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5551
Fragments arranged by size:
5551 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2777 2855 1186
Size: 988 603 78 4107
Fragments arranged by size:
4107 988 603 78
BseYI C'CCAG_C
Cuts at: 2202 3004 4979 5475 2202
Size: 802 1975 496 2503
Fragments arranged by size:
2503 1975 802 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2639 1163
Size: 573 903 4300
Fragments arranged by size:
4300 903 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2241 2847 3061 3837 3922 4993 2241
Size: 606 214 776 85 1071 3024
Fragments arranged by size:
3024 1071 776 606 214 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2079 2241 2769 2847 3061 3095
Size: 399 253 36 162 528 78 214 34
Cuts at: 3095 3837 3922 4993 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 399 253 214 162
85 78 36 34
BspEI T'CCGG_A
Cuts at: 2153 5439 2153
Size: 3286 2490
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2389 3131 844
Size: 1545 742 3489
Fragments arranged by size:
3489 1545 742
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2555 2603 2909 3836 4848 2555
Size: 48 306 927 1012 3483
Fragments arranged by size:
3483 1012 927 306 48
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2980 794
Size: 2186 3590
BstBI TT'CG_AA
Cuts at: 2856 4580 2856
Size: 1724 4052
BstEII G'GTnAC_C
Cuts at: 1184 2401 1184
Size: 1217 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 3004 3004
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 3048 3106 3473 3858 3875 5327 5413 5425
Size: 1076 58 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1076 385 367 346 86 58
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2095 513
Size: 552 939 8 83 4194
Fragments arranged by size:
4194 939 552 83 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2361 3223
Size: 54 1425 50 275 18 402 129 862
Cuts at: 3223 4112 5741 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 862 402 275 129 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3706 1353
Size: 719 1634 3423
Fragments arranged by size:
3423 1634 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2791 3007 4363 4737 4883 387
Size: 1064 138 1202 216 1356 374 146 1280
Fragments arranged by size:
1356 1280 1202 1064 374 216 146 138
EcoICRI GAG'CTC
Cuts at: 2239 2845 3059 2239
Size: 606 214 4956
Fragments arranged by size:
4956 606 214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2594 2696 2846 2918 3005 3037
Size: 393 641 321 102 150 72 87 32
Cuts at: 3037 3839 4436 5223 5491 1239
Size: 802 597 787 268 1524
Fragments arranged by size:
1524 802 787 641 597 393 321 268
150 102 87 72 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2984 2984
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2076 2704 2788 3034 2076
Size: 628 84 246 4818
Fragments arranged by size:
4818 628 246 84
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FspI TGC'GCA
Cuts at: 2690 2720 2907 4282 2690
Size: 30 187 1375 4184
Fragments arranged by size:
4184 1375 187 30
HaeII r_GCGC'y
Cuts at: 1678 2012 2051 4923 1678
Size: 334 39 2872 2531
Fragments arranged by size:
2872 2531 334 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373
Size: 32 135 32 2845 32 42 32 2626
Fragments arranged by size:
2845 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2294 748
Size: 1546 4230
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3423
Fragments arranged by size:
3423 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3734 4093 4252 518
Size: 648 2568 359 159 2042
Fragments arranged by size:
2568 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2087 2188 2896 3192 3869 5017 5262
Size: 408 73 101 708 296 677 1148 245
Cuts at: 5262 1606
Size: 2120
Fragments arranged by size:
2120 1148 708 677 408 296 245 101
73
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4285
Fragments arranged by size:
4285 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2769 1348
Size: 1421 4355
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 1108
Size: 1599 4177
PfoI T'CCnGG_A
Cuts at: 2835 2835
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 3845 3877 5358 5390 2741
Size: 32 1072 32 1481 32 3127
Fragments arranged by size:
3127 1481 1072 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2788 3034 2788
Size: 246 5530
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2075 2075
Size: 5776
PstI C_TGCA'G
Cuts at: 830 3033 3128 830
Size: 2203 95 3478
Fragments arranged by size:
3478 2203 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2188 2896 2188
Size: 708 5068
SacI G_AGCT'C
Cuts at: 2241 2847 3061 2241
Size: 606 214 4956
Fragments arranged by size:
4956 606 214
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2072 2072
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2108 4024 5736 2108
Size: 1916 1712 2148
Fragments arranged by size:
2148 1916 1712
SfcI C'TryA_G
Cuts at: 826 1045 2740 3029 3124 4259 4940 5131 826
Size: 219 1695 289 95 1135 681 191 1471
Fragments arranged by size:
1695 1471 1135 681 289 219 191 95
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 5776
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 3880 4781 5043 820
Size: 197 926 903 1034 901 262 1553
Fragments arranged by size:
1553 1034 926 903 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2997 3215 513
Size: 552 939 993 218 3074
Fragments arranged by size:
3074 993 939 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2441 2606 3803 3820 3973 4158 4603
Size: 1255 861 165 1197 17 153 185 445
Cuts at: 4603 325
Size: 1498
Fragments arranged by size:
1498 1255 1197 861 445 185 165 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2559 4022
Size: 274 80 33 51 1443 129 453 1463
Cuts at: 4022 5734 96
Size: 1712 138
Fragments arranged by size:
1712 1463 1443 453 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2450 2482 5736 5768 2450
Size: 32 3254 32 2458
Fragments arranged by size:
3254 2458 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2371
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SexAI SfiI SrfI SwaI