Restriction map for
pFN21AB6490
(Circular) (Six-base) MAPSORT of: pFN21AB6490.seq Check: 3434 from: 1 to: 5776
pFN21AB6490 5776 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2366 2366
Size: 5776
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2382 2700 3119 1937
Size: 445 318 419 4594
Fragments arranged by size:
4594 445 419 318
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 3839 5223 5491 1632
Size: 515 1692 1384 268 1917
Fragments arranged by size:
1917 1692 1384 515 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 2297 1166
Size: 1131 4645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 2007
Size: 32 5744
AlwNI CAG_nnn'CTG
Cuts at: 3049 3519 5091 3049
Size: 470 1572 3734
Fragments arranged by size:
3734 1572 470
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 1870
Size: 73 1158 4545
Fragments arranged by size:
4545 1158 73
BamHI G'GATC_C
Cuts at: 2040 3106 3473 2040
Size: 1066 367 4343
Fragments arranged by size:
4343 1066 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2351 3095 1391
Size: 960 744 4072
Fragments arranged by size:
4072 960 744
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2959 928
Size: 971 1060 3745
Fragments arranged by size:
3745 1060 971
BbvCI CC'TCA_GC
Cuts at: 2891 2891
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2266 3668 4878 2266
Size: 1402 1210 3164
Fragments arranged by size:
3164 1402 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2355 4387 136
Size: 107 122 71 631 605 683 2032 1525
Fragments arranged by size:
2032 1525 683 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2304 2372 2804 3006 3837 4993 1790
Size: 514 68 432 202 831 1156 2573
Fragments arranged by size:
2573 1156 831 514 432 202 68
BmgBI CAC'GTC
Cuts at: 1318 2926 1318
Size: 1608 4168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2702 3507 4465
Size: 416 53 83 399 24 1285 805 958
Cuts at: 4465 442
Size: 1753
Fragments arranged by size:
1753 1285 958 805 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2231 2231
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2574 2738 2818 4436 1239
Size: 806 529 164 80 1618 2579
Fragments arranged by size:
2579 1618 806 529 164 80
Bpu10I CC'TnA_GC
Cuts at: 2891 2891
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2669 2774 3901 4766 5064 2606
Size: 63 105 1127 865 298 3318
Fragments arranged by size:
3318 1127 865 298 105 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2389 3567 493
Size: 711 1185 1178 2702
Fragments arranged by size:
2702 1185 1178 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2063 2073 2093 1178
Size: 30 165 30 640 20 10 20 4861
Fragments arranged by size:
4861 640 165 30 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2777 1186
Size: 1591 4185
BseYI C'CCAG_C
Cuts at: 4979 5475 4979
Size: 496 5280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 2906 1163
Size: 573 774 396 4033
Fragments arranged by size:
4033 774 573 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3837 3922 4993 3837
Size: 85 1071 4620
Fragments arranged by size:
4620 1071 85
BsmI GAATG_Cn'
Cuts at: 2131 3287 3380 2131
Size: 1156 93 4527
Fragments arranged by size:
4527 1156 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2351 2372 2804 3006 3095 3837
Size: 399 514 47 21 432 202 89 742
Cuts at: 3837 3922 4993 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 432 399 202 89
85 47 21
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2366 3131 844
Size: 1522 765 3489
Fragments arranged by size:
3489 1522 765
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2568 4271 4445 65
Size: 1183 1320 1703 174 1396
Fragments arranged by size:
1703 1396 1320 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 2841 96
Size: 2745 3031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2036 2262 2036
Size: 226 5550
BstYI r'GATC_y
Cuts at: 1972 2040 3106 3473 3858 3875 5327 5413 5425
Size: 68 1066 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1066 385 367 346 86 68
17 12
BstZ17I GTA'TAC
Cuts at: 2383 2383
Size: 5776
Bsu36I CC'TnA_GG
Cuts at: 2120 2120
Size: 5776
BtgI C'CryG_G
Cuts at: 513 1065 2004 2364 513
Size: 552 939 360 3925
Fragments arranged by size:
3925 939 552 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2758 2793 529
Size: 35 2194 35 3512
Fragments arranged by size:
3512 2194 35 35
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 3223 4112
Size: 54 1425 50 275 18 526 867 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 867 526 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 3706 1353
Size: 794 1559 3423
Fragments arranged by size:
3423 1559 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2876 4363 4737 4883 387
Size: 1064 138 1287 1487 374 146 1280
Fragments arranged by size:
1487 1287 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2147 2574 2738 2818 3839 4436
Size: 393 413 102 427 164 80 1021 597
Cuts at: 4436 5223 5491 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1021 787 597 427 413 393 268
164 102 80
EcoNI CCTnn'n_nnAGG
Cuts at: 3041 3041
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 3046 3046
Size: 5776
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 4923 1678
Size: 3245 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373
Size: 32 135 32 2845 32 42 32 2626
Fragments arranged by size:
2845 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2049 3120 3366 669
Size: 988 281 111 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2358 10
Size: 54 1425 343 526 3428
Fragments arranged by size:
3428 1425 526 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2260 2297 3734 4093 4252 518
Size: 648 933 161 37 1437 359 159 2042
Fragments arranged by size:
2042 1437 933 648 359 161 159 37
MspA1I CmG'CkG
Cuts at: 1606 2494 3192 3869 5017 5262 1606
Size: 888 698 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 698 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2364 513
Size: 552 939 360 3925
Fragments arranged by size:
3925 939 552 360
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2842 3134 4679 1132
Size: 97 1613 292 1545 2229
Fragments arranged by size:
2229 1613 1545 292 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2365 3049 1108
Size: 1257 684 3835
Fragments arranged by size:
3835 1257 684
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3046 3046
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 3128 830
Size: 2298 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2494 2494
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2284 2440 3124 4259 4940 5131 826
Size: 219 1239 156 684 1135 681 191 1471
Fragments arranged by size:
1471 1239 1135 684 681 219 191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 2684 2789 3880 4781 5043
Size: 197 926 678 63 105 1091 901 262
Cuts at: 5043 820
Size: 1553
Fragments arranged by size:
1553 1091 926 901 678 262 197 105
63
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 2630 3700 5
Size: 47 2578 1070 2081
Fragments arranged by size:
2578 2081 1070 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 3215 513
Size: 552 939 360 851 3074
Fragments arranged by size:
3074 939 851 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2684 3803 3820 3973 4158 4603 325
Size: 1255 1104 1119 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1119 1104 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 4022 5734
Size: 274 80 33 51 1443 864 1181 1712
Cuts at: 5734 96
Size: 138
Fragments arranged by size:
1712 1443 1181 864 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2043 2075 5736 5768 2043
Size: 32 3661 32 2051
Fragments arranged by size:
3661 2051 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2250 1316
Size: 934 4842
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 2129 3905 2129
Size: 1776 4000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI