Restriction map for
pFN21AB6489
(Circular) (Six-base) MAPSORT of: pFN21AB6489.seq Check: 1459 from: 1 to: 5692
pFN21AB6489 5692 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2033 2600 2033
Size: 567 5125
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 2017 3013 2017
Size: 996 4696
AclI AA'CG_TT
Cuts at: 1563 2983 3819 4192 1563
Size: 1420 836 373 3063
Fragments arranged by size:
3063 1420 836 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2566 3755 5139 5407 1632
Size: 731 203 1189 1384 268 1917
Fragments arranged by size:
1917 1384 1189 731 268 203
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 2371 2408 4591 5568 1203
Size: 1168 37 2183 977 1327
Fragments arranged by size:
2183 1327 1168 977 37
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2030 2780 2870 3435 5007 2030
Size: 750 90 565 1572 2715
Fragments arranged by size:
2715 1572 750 565 90
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2173 2206 2173
Size: 33 5659
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 3013 4468 618
Size: 325 217 627 230 996 1455 1842
Fragments arranged by size:
1842 1455 996 627 325 230 217
BanII G_rGCy'C
Cuts at: 1391 3011 1391
Size: 1620 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2484 928
Size: 971 585 4136
Fragments arranged by size:
4136 971 585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2174 2246 3584 4794 2174
Size: 72 1338 1210 3072
Fragments arranged by size:
3072 1338 1210 72
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2969 5687 2969
Size: 2718 2974
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 3753 4909 1790
Size: 1963 1156 2573
Fragments arranged by size:
2573 1963 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 3423 4381
Size: 416 53 83 399 24 779 1227 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1227 958 779 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 4352 1239
Size: 1412 1701 2579
Fragments arranged by size:
2579 1701 1412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2569 4355 882
Size: 617 1070 1786 2219
Fragments arranged by size:
2219 1786 1070 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2247 2832 4125 4523 4797 4944 5355 2247
Size: 585 1293 398 274 147 411 2584
Fragments arranged by size:
2584 1293 585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2624 2654 1178
Size: 30 165 30 1221 30 4216
Fragments arranged by size:
4216 1221 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 1186
Size: 896 4796
BseYI C'CCAG_C
Cuts at: 4895 5391 4895
Size: 496 5196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 2710 1163
Size: 573 921 53 4145
Fragments arranged by size:
4145 921 573 53
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 2506 3203 3296 2506
Size: 697 93 4902
Fragments arranged by size:
4902 697 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3011 3753 3838 4909 1391
Size: 399 1221 742 85 1071 2174
Fragments arranged by size:
2174 1221 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2832 5355 2832
Size: 2523 3169
BspHI T'CATG_A
Cuts at: 2562 3581 5311 2562
Size: 1019 1730 2943
Fragments arranged by size:
2943 1730 1019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 2600 3047 844
Size: 1189 567 447 3489
Fragments arranged by size:
3489 1189 567 447
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2865 4187 4361 65
Size: 1183 1617 1322 174 1396
Fragments arranged by size:
1617 1396 1322 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 2700 96
Size: 2604 3088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2228 2298 2228
Size: 70 5622
BstYI r'GATC_y
Cuts at: 1972 2074 2969 3022 3389 3774 3791 5243 5329
Size: 102 895 53 367 385 17 1452 86
Cuts at: 5329 5341 5687 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 895 385 367 346 102 86
53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2645 3208 4002 4022 2645
Size: 563 794 20 4315
Fragments arranged by size:
4315 794 563 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657
Size: 54 1425 50 275 18 1309 889 1629
Cuts at: 5657 8
Size: 43
Fragments arranged by size:
1629 1425 1309 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2566 2651 3755 4352 5139 5407
Size: 393 731 203 85 1104 597 787 268
Cuts at: 5407 1239
Size: 1524
Fragments arranged by size:
1524 1104 787 731 597 393 268 203
85
EcoO109I rG'GnC_Cy
Cuts at: 2123 2520 2123
Size: 397 5295
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2780 2795 2812 2827 4333
Size: 32 135 32 1208 15 17 15 1506
Cuts at: 4333 4365 4407 4439 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1506 1208 135 42 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 2021 3017 2021
Size: 996 4696
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 2838 4806 4990 1305
Size: 1035 498 1968 184 2007
Fragments arranged by size:
2007 1968 1035 498 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 3650 4009 4168 518
Size: 648 1401 1083 359 159 2042
Fragments arranged by size:
2042 1401 1083 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2138 3108 3785 4933 5178 1606
Size: 532 970 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 677 532 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2375 2412 3050 4595 1132
Size: 97 1146 37 638 1545 2229
Fragments arranged by size:
2229 1545 1146 638 97 37
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 2371 2408 4591 2371
Size: 37 2183 3472
Fragments arranged by size:
3472 2183 37
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2153 2231 1108
Size: 1045 78 4569
Fragments arranged by size:
4569 1045 78
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2123 2123
Size: 5692
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 2044 3044 830
Size: 1214 1000 3478
Fragments arranged by size:
3478 1214 1000
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2138 2138
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 2044 3044 2044
Size: 1000 4692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2040 2185 3040 4175 4856 5047 826
Size: 219 995 145 855 1135 681 191 1471
Fragments arranged by size:
1471 1135 995 855 681 219 191 145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2083 1517
Size: 566 5126
StyI C'CwwG_G
Cuts at: 513 1065 2078 3131 513
Size: 552 1013 1053 3074
Fragments arranged by size:
3074 1053 1013 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 3938 5650
Size: 274 80 33 51 1443 723 1238 1712
Cuts at: 5650 96
Size: 138
Fragments arranged by size:
1712 1443 1238 723 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2624 2656 5652 5684 2624
Size: 32 2996 32 2632
Fragments arranged by size:
2996 2632 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2228 2626 2011
Size: 217 398 5077
Fragments arranged by size:
5077 398 217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI