Restriction map for
pFN21AB6484
(Circular) (Six-base) MAPSORT of: pFN21AB6484.seq Check: 6629 from: 1 to: 5773
pFN21AB6484 5773 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 2968 3116 1937
Size: 537 494 148 4594
Fragments arranged by size:
4594 537 494 148
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3836 5220 5488 1632
Size: 2204 1384 268 1917
Fragments arranged by size:
2204 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 2586 4672 5649 1203
Size: 1383 2086 977 1327
Fragments arranged by size:
2086 1383 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 2141 3516 5088 2141
Size: 1375 1572 2826
Fragments arranged by size:
2826 1572 1375
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 3017 3082 3308 3460 5569 1493
Size: 1524 65 226 152 2109 1697
Fragments arranged by size:
2109 1697 1524 226 152 65
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 3098 1870
Size: 73 1155 4545
Fragments arranged by size:
4545 1155 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3094 4549 618
Size: 325 217 627 1307 1455 1842
Fragments arranged by size:
1842 1455 1307 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3092 1391
Size: 1701 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4802
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3052 3086 3964 3998 1903
Size: 34 1115 34 878 34 3678
Fragments arranged by size:
3678 1115 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4384 136
Size: 107 122 71 631 605 2712 1525
Fragments arranged by size:
2712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 2436 3190 2436
Size: 754 5019
Bme1580I G_kGCm'C
Cuts at: 1790 3834 4990 1790
Size: 2044 1156 2573
Fragments arranged by size:
2573 2044 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4433 1239
Size: 3194 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2174 2587 3564 493
Size: 711 970 413 977 2702
Fragments arranged by size:
2702 977 970 711 413
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3580 3962 4597 275
Size: 53 83 186 936 2047 382 635 1451
Fragments arranged by size:
2047 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2799 2829 1178
Size: 30 165 30 1396 30 4122
Fragments arranged by size:
4122 1396 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5773
BseYI C'CCAG_C
Cuts at: 2076 2564 2974 4976 5472 2076
Size: 488 410 2002 496 2377
Fragments arranged by size:
2377 2002 496 488 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 1163
Size: 573 378 4822
Fragments arranged by size:
4822 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3834 3919 4990 3834
Size: 85 1071 4617
Fragments arranged by size:
4617 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3092 3834 3919 4990 1391
Size: 399 1302 742 85 1071 2174
Fragments arranged by size:
2174 1302 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2486 3128 844
Size: 1642 642 3489
Fragments arranged by size:
3489 1642 642
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 4268 4442 65
Size: 1183 1014 2006 174 1396
Fragments arranged by size:
2006 1396 1183 1014 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4417 4604 1700
Size: 110 2607 187 2869
Fragments arranged by size:
2869 2607 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 794
Size: 1652 4121
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstXI CCAn_nnnn'nTGG
Cuts at: 3022 3022
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2457 513
Size: 552 1392 3829
Fragments arranged by size:
3829 1392 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 2102 3289 4083 4103 2102
Size: 1187 794 20 3772
Fragments arranged by size:
3772 1187 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 2392 3077 2392
Size: 685 5088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2321 4780 809
Size: 507 1005 2459 1802
Fragments arranged by size:
2459 1802 1005 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3012 3220 4109
Size: 54 1425 50 275 18 1182 208 889
Cuts at: 4109 5738 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1182 889 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3703 1353
Size: 2350 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4360 4734 4880 387
Size: 1064 138 2771 374 146 1280
Fragments arranged by size:
2771 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3836 4433 5220 5488 1239
Size: 393 2204 597 787 268 1524
Fragments arranged by size:
2204 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2884 2884
Size: 5773
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2702 2702
Size: 5773
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 4920 1678
Size: 3242 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4414 4446 4488 4520 1373
Size: 32 135 32 2842 32 42 32 2626
Fragments arranged by size:
2842 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 3117 3363 669
Size: 988 281 478 701 246 3079
Fragments arranged by size:
3079 988 701 478 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2170 4887 5071 1305
Size: 865 2717 184 2007
Fragments arranged by size:
2717 2007 865 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 10
Size: 54 1425 343 1182 2769
Fragments arranged by size:
2769 1425 1182 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2910 3731 4090 4249 518
Size: 648 1744 821 359 159 2042
Fragments arranged by size:
2042 1744 821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2974 3189 3866 5014 5259 1606
Size: 474 894 215 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 474 245 215
NcoI C'CATG_G
Cuts at: 513 1065 2457 513
Size: 552 1392 3829
Fragments arranged by size:
3829 1392 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 2444 3131 4676 1132
Size: 97 1215 687 1545 2229
Fragments arranged by size:
2229 1545 1215 687 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PmlI CAC'GTG
Cuts at: 2587 2587
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3033 3343 3033
Size: 310 5463
PstI C_TGCA'G
Cuts at: 830 2483 2705 2731 3125 830
Size: 1653 222 26 394 3478
Fragments arranged by size:
3478 1653 394 222 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2080 2974 2080
Size: 894 4879
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SexAI A'CCwGG_T
Cuts at: 2088 2088
Size: 5773
SfcI C'TryA_G
Cuts at: 826 1045 2479 2701 2727 2969 3121 4256 4937
Size: 219 1434 222 26 242 152 1135 681
Cuts at: 4937 5128 826
Size: 191 1471
Fragments arranged by size:
1471 1434 1135 681 242 222 219 191
152 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3100 1872
Size: 1228 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3877 4778 5040 820
Size: 197 926 1934 901 262 1553
Fragments arranged by size:
1934 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 2444 3131 1132
Size: 1312 687 3774
Fragments arranged by size:
3774 1312 687
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2040 2457 3212 513
Size: 552 975 417 755 3074
Fragments arranged by size:
3074 975 755 552 417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2005 2331 3800 3817 3970 4155 4600
Size: 1255 425 326 1469 17 153 185 445
Cuts at: 4600 325
Size: 1498
Fragments arranged by size:
1498 1469 1255 445 425 326 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4019 5731 96
Size: 274 80 33 51 1443 2042 1712 138
Fragments arranged by size:
2042 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2799 2831 5733 5765 2799
Size: 32 2902 32 2807
Fragments arranged by size:
2902 2807 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2995 2995
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3098 1870
Size: 1228 4545
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI