Restriction map for
pFN21AB6483
(Circular) (Six-base) MAPSORT of: pFN21AB6483.seq Check: 5209 from: 1 to: 5689
pFN21AB6483 5689 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2865 2865
Size: 5689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2373 2925 4588 5565 1203
Size: 1170 552 1663 977 1327
Fragments arranged by size:
1663 1327 1170 977 552
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2839 3432 5004 2839
Size: 593 1572 3524
Fragments arranged by size:
3524 1572 593
ApaI G_GGCC'C
Cuts at: 2059 2059
Size: 5689
ApaLI G'TGCA_C
Cuts at: 2105 3746 4902 2105
Size: 1641 1156 2892
Fragments arranged by size:
2892 1641 1156
ApoI r'AATT_y
Cuts at: 1493 2204 2456 2998 3224 3376 5485 1493
Size: 711 252 542 226 152 2109 1697
Fragments arranged by size:
2109 1697 711 542 252 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2502 2556 2585 3014 1870
Size: 73 559 54 29 429 4545
Fragments arranged by size:
4545 559 429 73 54 29
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2781 2973 3010 4465 618
Size: 325 217 627 994 192 37 1455 1842
Fragments arranged by size:
1842 1455 994 627 325 217 192 37
BanII G_rGCy'C
Cuts at: 1391 2059 3008 1391
Size: 668 949 4072
Fragments arranged by size:
4072 949 668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2091 4300 136
Size: 107 122 71 631 605 419 2209 1525
Fragments arranged by size:
2209 1525 631 605 419 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 2894 3106 2894
Size: 212 5477
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2109 2784 3750 4906 1790
Size: 269 50 675 966 1156 2573
Fragments arranged by size:
2573 1156 966 675 269 50
BmgBI CAC'GTC
Cuts at: 1318 2467 1318
Size: 1149 4540
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2818 3420 4378
Size: 416 53 83 399 24 1401 602 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1401 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2148 2148
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 4349 1239
Size: 1412 1698 2579
Fragments arranged by size:
2579 1698 1412
Bpu10I CC'TnA_GC
Cuts at: 2123 2123
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2474 4352 882
Size: 617 975 1878 2219
Fragments arranged by size:
2219 1878 975 617
BsaAI yAC'GTr
Cuts at: 493 1204 2376 3480 493
Size: 711 1172 1104 2702
Fragments arranged by size:
2702 1172 1104 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 3496 3878 4513
Size: 53 83 186 936 612 1351 382 635
Cuts at: 4513 275
Size: 1451
Fragments arranged by size:
1451 1351 936 635 612 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2708 2738 1178
Size: 30 165 30 1305 30 4129
Fragments arranged by size:
4129 1305 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2576 2603 2687 2726 2729 1186
Size: 1240 150 27 84 39 3 4146
Fragments arranged by size:
4146 1240 150 84 39 27 3
BseYI C'CCAG_C
Cuts at: 2289 4892 5388 2289
Size: 2603 496 2590
Fragments arranged by size:
2603 2590 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 2750 2846 1163
Size: 573 828 186 96 4006
Fragments arranged by size:
4006 828 573 186 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 3750 3835 4906 2109
Size: 1641 85 1071 2892
Fragments arranged by size:
2892 1641 1071 85
BsiWI C'GTAC_G
Cuts at: 2376 2376
Size: 5689
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2109 2784 3008 3750 3835 4906
Size: 399 269 50 675 224 742 85 1071
Cuts at: 4906 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 675 399 269 224 85
50
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2461 3578 5308 2461
Size: 1117 1730 2842
Fragments arranged by size:
2842 1730 1117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2865 3044 844
Size: 2021 179 3489
Fragments arranged by size:
3489 2021 179
BsrBI CCG'CTC
Cuts at: 1394 1823 2512 3576 1394
Size: 429 689 1064 3507
Fragments arranged by size:
3507 1064 689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2719 4333 4520 1700
Size: 110 909 1614 187 2869
Fragments arranged by size:
2869 1614 909 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2864 3749 4761 2864
Size: 885 1012 3792
Fragments arranged by size:
3792 1012 885
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2686 2764 3019 3386 3771 3788 5240 5326
Size: 714 78 255 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 714 385 367 346 255 86
78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2149 513
Size: 552 1084 4053
Fragments arranged by size:
4053 1084 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2665 487
Size: 683 56 1439 3511
Fragments arranged by size:
3511 1439 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DraIII CAC_nnn'GTG
Cuts at: 2812 2812
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2196 2301
Size: 54 1425 50 275 18 262 104 105
Cuts at: 2301 2434 2526 2550 3136 4025 5654 8
Size: 133 92 24 586 889 1629 43
Fragments arranged by size:
1629 1425 889 586 275 262 133 105
104 92 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2263 4276 4650 4796 387
Size: 1064 138 674 2013 374 146 1280
Fragments arranged by size:
2013 1280 1064 674 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 3752 4349 5136 5404 1239
Size: 393 1019 1101 597 787 268 1524
Fragments arranged by size:
1524 1101 1019 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2055 2084 2635 2962 2055
Size: 29 551 327 4782
Fragments arranged by size:
4782 551 327 29
EcoRI G'AATT_C
Cuts at: 2204 2998 3376 2204
Size: 794 378 4517
Fragments arranged by size:
4517 794 378
FspI TGC'GCA
Cuts at: 2420 4195 2420
Size: 1775 3914
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2517 2528 2539 2560
Size: 32 135 32 913 32 11 11 21
Cuts at: 2560 2571 2678 2710 4330 4362 4404 4436 1373
Size: 11 107 32 1620 32 42 32 2626
Fragments arranged by size:
2626 1620 913 135 107 42 32 32
32 32 32 32 21 11 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2094 2198 2436 2552 10
Size: 54 1425 343 262 104 238 116 3147
Fragments arranged by size:
3147 1425 343 262 238 116 104 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2787 2930 2979 3647 4006 4165 518
Size: 648 1621 143 49 668 359 159 2042
Fragments arranged by size:
2042 1621 668 648 359 159 143 49
MspA1I CmG'CkG
Cuts at: 1606 2338 3105 3782 4930 5175 1606
Size: 732 767 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 732 677 245
NaeI GCC'GGC
Cuts at: 2721 2721
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2719 2719
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2605 2929 3047 4592 1132
Size: 97 1376 324 118 1545 2229
Fragments arranged by size:
2229 1545 1376 324 118 97
PasI CC'CwG_GG
Cuts at: 1348 2059 1348
Size: 711 4978
PciI A'CATG_T
Cuts at: 2925 4588 2925
Size: 1663 4026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2839 1108
Size: 902 829 3958
Fragments arranged by size:
3958 902 829
PfoI T'CCnGG_A
Cuts at: 2751 2760 2751
Size: 9 5680
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2635 2962 2635
Size: 327 5362
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2055 2055
Size: 5689
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2147 2179 2147
Size: 32 5657
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
RsrII CG'GwC_CG
Cuts at: 2386 2386
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 3037 4172 4853 5044 826
Size: 219 1992 1135 681 191 1471
Fragments arranged by size:
1992 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2091 2091
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2556 3793 4694 4956 820
Size: 197 926 613 1237 901 262 1553
Fragments arranged by size:
1553 1237 926 901 613 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2199 3128 513
Size: 552 1134 929 3074
Fragments arranged by size:
3074 1134 929 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2281 2716 2800 3716 3733 3886 4071
Size: 1255 701 435 84 916 17 153 185
Cuts at: 4071 4516 325
Size: 445 1498
Fragments arranged by size:
1498 1255 916 701 445 435 185 153
84 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2676 2691 3935
Size: 274 80 33 51 1443 699 15 1244
Cuts at: 3935 5647 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1244 699 274 138 80 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 2708 2740 5649 5681 2178
Size: 32 498 32 2909 32 2186
Fragments arranged by size:
2909 2186 498 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 2556 1943
Size: 613 5076
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 2457 3818 2457
Size: 1361 4328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
RsrII SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PvuII SacI SacII SanDI SapI SexAI SfoI
SrfI SwaI XcmI