Restriction map for
pFN21AB6481
(Circular) (Six-base) MAPSORT of: pFN21AB6481.seq Check: 516 from: 1 to: 5638
pFN21AB6481 5638 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3701 5085 5353 1632
Size: 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 2364 4469 2364
Size: 2105 3533
AhdI GACnn_n'nnGTC
Cuts at: 2353 4367 2353
Size: 2014 3624
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 3940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2218 3381 4953 2218
Size: 1163 1572 2903
Fragments arranged by size:
2903 1572 1163
ApaI G_GGCC'C
Cuts at: 2507 2507
Size: 5638
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 2963 1870
Size: 73 741 279 4545
Fragments arranged by size:
4545 741 279 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2361 2372 2959 4414 618
Size: 325 217 627 574 11 587 1455 1842
Fragments arranged by size:
1842 1455 627 587 574 325 217 11
BanII G_rGCy'C
Cuts at: 1391 2507 2685 2957 1391
Size: 1116 178 272 4072
Fragments arranged by size:
4072 1116 272 178
BbeI G_GCGC'C
Cuts at: 2376 2376
Size: 5638
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2549 4249 136
Size: 107 122 71 631 605 877 1700 1525
Fragments arranged by size:
1700 1525 877 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2507 2693 2778 3699 4855 1790
Size: 717 186 85 921 1156 2573
Fragments arranged by size:
2573 1156 921 717 186 85
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2871 3369 4327
Size: 416 53 83 399 24 1454 498 958
Cuts at: 4327 442
Size: 1753
Fragments arranged by size:
1753 1454 958 498 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 2884 4298 1239
Size: 1565 80 1414 2579
Fragments arranged by size:
2579 1565 1414 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 2340 4301 882
Size: 617 627 214 1961 2219
Fragments arranged by size:
2219 1961 627 617 214
BsaAI yAC'GTr
Cuts at: 493 1204 2206 2671 3429 493
Size: 711 1002 465 758 2702
Fragments arranged by size:
2702 1002 758 711 465
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2373 2430 3445
Size: 53 83 186 936 482 358 57 1015
Cuts at: 3445 3827 4462 275
Size: 382 635 1451
Fragments arranged by size:
1451 1015 936 635 482 382 358 186
83 57 53
BsaWI w'CCGG_w
Cuts at: 2125 2364 4071 4469 4743 4890 5301 2125
Size: 239 1707 398 274 147 411 2462
Fragments arranged by size:
2462 1707 411 398 274 239 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 2278 2308 2660
Size: 30 165 30 633 30 212 30 352
Cuts at: 2660 2690 1178
Size: 30 4126
Fragments arranged by size:
4126 633 352 212 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2573 2678 2866 2869 2887 1186
Size: 946 441 105 188 3 18 3937
Fragments arranged by size:
3937 946 441 188 105 18 3
BseYI C'CCAG_C
Cuts at: 2495 2716 2909 4841 5337 2495
Size: 221 193 1932 496 2796
Fragments arranged by size:
2796 1932 496 221 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 1163
Size: 573 768 4297
Fragments arranged by size:
4297 768 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2419 3088 3849 4877 1540
Size: 275 186 418 669 761 1028 2301
Fragments arranged by size:
2301 1028 761 669 418 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 2205 2670 3699 3784 4855 2144
Size: 61 465 1029 85 1071 2927
Fragments arranged by size:
2927 1071 1029 465 85 61
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2205 2507 2670 2685 2693 2778
Size: 399 354 61 302 163 15 8 85
Cuts at: 2778 2957 3699 3784 4855 1391
Size: 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 354 302 179 163
85 85 61 15 8
BspEI T'CCGG_A
Cuts at: 2125 5301 2125
Size: 3176 2462
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 2334 2364 2414 2418 2614 4282
Size: 110 377 147 30 50 4 196 1668
Cuts at: 4282 4469 1700
Size: 187 2869
Fragments arranged by size:
2869 1668 377 196 187 147 110 50
30 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2560 2560
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2280 2968 3335 3720 3737 5189 5275 5287
Size: 308 688 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 688 385 367 346 308 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 513
Size: 552 939 51 4096
Fragments arranged by size:
4096 939 552 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 3154 3948 3968 2044
Size: 1110 794 20 3714
Fragments arranged by size:
3714 1110 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2189 2416
Size: 54 1425 50 275 18 177 182 227
Cuts at: 2416 2838 3085 3974 5603 8
Size: 422 247 889 1629 43
Fragments arranged by size:
1629 1425 889 422 275 247 227 182
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2416 3085 1537
Size: 275 604 669 4090
Fragments arranged by size:
4090 669 604 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 3568 1353
Size: 771 1444 3423
Fragments arranged by size:
3423 1444 771
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2804 2884 3701 4298 5085 5353 1239
Size: 393 1172 80 817 597 787 268 1524
Fragments arranged by size:
1524 1172 817 787 597 393 268 80
EcoO109I rG'GnC_Cy
Cuts at: 2323 2504 2606 2638 2323
Size: 181 102 32 5323
Fragments arranged by size:
5323 181 102 32
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 2376 4785 1678
Size: 698 2409 2531
Fragments arranged by size:
2531 2409 698
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2335 2339 2367 2371 4279
Size: 32 135 32 763 4 28 4 1908
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1908 763 135 42 32 32 32
32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2467 2982 3228 669
Size: 988 281 529 515 246 3079
Fragments arranged by size:
3079 988 529 515 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KasI G'GCGC_C
Cuts at: 2372 2372
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 4752 4936 1305
Size: 873 2574 184 2007
Fragments arranged by size:
2574 2007 873 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3816
Fragments arranged by size:
3816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2864 3596 3955 4114 518
Size: 648 1698 732 359 159 2042
Fragments arranged by size:
2042 1698 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2737 2799 3054 3731 4879 5124 1606
Size: 1131 62 255 677 1148 245 2120
Fragments arranged by size:
2120 1148 1131 677 255 245 62
NaeI GCC'GGC
Cuts at: 2189 2336 2420 2189
Size: 147 84 5407
Fragments arranged by size:
5407 147 84
NarI GG'CG_CC
Cuts at: 2373 2373
Size: 5638
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NgoMIV G'CCGG_C
Cuts at: 2187 2334 2418 2187
Size: 147 84 5407
Fragments arranged by size:
5407 147 84
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2508 1348
Size: 1160 4478
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PfoI T'CCnGG_A
Cuts at: 2026 2026
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PmlI CAC'GTG
Cuts at: 2206 2206
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2323
Size: 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PspOMI G'GGCC_C
Cuts at: 2503 2503
Size: 5638
PspXI vC'TCGA_Gb
Cuts at: 2684 2684
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2737 2799 2737
Size: 62 5576
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SanDI GG'GwC_CC
Cuts at: 2323 2323
Size: 5638
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2986 4121 4802 4993 826
Size: 219 1941 1135 681 191 1471
Fragments arranged by size:
1941 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2374 2374
Size: 5638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 3742 4643 4905 820
Size: 197 926 741 1058 901 262 1553
Fragments arranged by size:
1553 1058 926 901 741 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 3077 513
Size: 552 939 1073 3074
Fragments arranged by size:
3074 1073 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2708 3665 3682 3835 4020 4465 325
Size: 1255 1128 957 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1128 957 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3884 5596 96
Size: 274 80 33 51 1443 1907 1712 138
Fragments arranged by size:
1907 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2278 2310 2660 2692 5598 5630 2036
Size: 32 210 32 350 32 2906 32 2044
Fragments arranged by size:
2906 2044 350 210 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2497
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 2684 1943
Size: 741 4897
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI