Restriction map for
pFN21AB6480
(Circular) (Six-base) MAPSORT of: pFN21AB6480.seq Check: 6032 from: 1 to: 5716
pFN21AB6480 5716 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2952 3526 278
Size: 53 83 186 936 1416 574 2468
Fragments arranged by size:
2468 1416 936 574 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2190 3059 1937
Size: 253 869 4594
Fragments arranged by size:
4594 869 253
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2723 3779 5163 5431 1632
Size: 1091 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1091 1056 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 2242 2962 4445 2242
Size: 720 1483 3513
Fragments arranged by size:
3513 1483 720
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 2143 3459 5031 2143
Size: 1316 1572 2828
Fragments arranged by size:
2828 1572 1316
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 3025 3251 3403 5512 1493
Size: 1532 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3037 4492 618
Size: 325 217 627 1250 1455 1842
Fragments arranged by size:
1842 1455 1250 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3035 1391
Size: 1644 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 928
Size: 971 142 4603
Fragments arranged by size:
4603 971 142
BbvCI CC'TCA_GC
Cuts at: 2103 2308 2103
Size: 205 5511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3907 3941 1903
Size: 34 50 34 1886 34 3678
Fragments arranged by size:
3678 1886 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 4327 136
Size: 107 122 71 631 605 394 2261 1525
Fragments arranged by size:
2261 1525 631 605 394 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 2355 3133 2355
Size: 778 4938
Bme1580I G_kGCm'C
Cuts at: 1790 3777 4933 1790
Size: 1987 1156 2573
Fragments arranged by size:
2573 1987 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2551 3447 4405
Size: 416 53 83 399 24 1134 896 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1134 958 896 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 2602 4376 1239
Size: 1176 187 1774 2579
Fragments arranged by size:
2579 1774 1176 187
Bpu10I CC'TnA_GC
Cuts at: 2103 2165 2308 2103
Size: 62 143 5511
Fragments arranged by size:
5511 143 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2949 3523 3905 4540
Size: 53 83 186 936 1416 574 382 635
Cuts at: 4540 275
Size: 1451
Fragments arranged by size:
1451 1416 936 635 574 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2980 4149 4547 4821 4968 5379 2980
Size: 1169 398 274 147 411 3317
Fragments arranged by size:
3317 1169 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2091 2473 1186
Size: 905 382 4429
Fragments arranged by size:
4429 905 382
BseYI C'CCAG_C
Cuts at: 2088 2359 4919 5415 2088
Size: 271 2560 496 2389
Fragments arranged by size:
2560 2389 496 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 1163
Size: 573 483 4660
Fragments arranged by size:
4660 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2012 2235 3166 3927 4955 1540
Size: 275 186 11 223 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 223 186 11
BsiHKAI G_wGCw'C
Cuts at: 2368 3777 3862 4933 2368
Size: 1409 85 1071 3151
Fragments arranged by size:
3151 1409 1071 85
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2368 3035 3777 3862 4933 1391
Size: 399 578 667 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 667 578 399 85
BspEI T'CCGG_A
Cuts at: 2980 5379 2980
Size: 2399 3317
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2128 3071 844
Size: 1284 943 3489
Fragments arranged by size:
3489 1284 943
BsrBI CCG'CTC
Cuts at: 1394 1823 2099 3603 1394
Size: 429 276 1504 3507
Fragments arranged by size:
3507 1504 429 276
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4360 4547 1700
Size: 110 2550 187 2869
Fragments arranged by size:
2869 2550 187 110
BsrGI T'GTAC_A
Cuts at: 96 2124 2261 96
Size: 2028 137 3551
Fragments arranged by size:
3551 2028 137
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 2176 3776 4788 2176
Size: 1600 1012 3104
Fragments arranged by size:
3104 1600 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 2446 1184
Size: 1262 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711
Size: 1074 367 385 17 1452 86 12 346
Cuts at: 5711 1972
Size: 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2064 2511 2760 513
Size: 552 999 447 249 3469
Fragments arranged by size:
3469 999 552 447 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2482 487
Size: 683 56 1256 3721
Fragments arranged by size:
3721 1256 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 3232 4026 4046 2292
Size: 940 794 20 3962
Fragments arranged by size:
3962 940 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 2206 2889 3020 2206
Size: 683 131 4902
Fragments arranged by size:
4902 683 131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 3163 4052
Size: 54 1425 50 275 18 402 931 889
Cuts at: 4052 5681 8
Size: 1629 43
Fragments arranged by size:
1629 1425 931 889 402 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2232 3163 1537
Size: 275 420 931 4090
Fragments arranged by size:
4090 931 420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2874 3646 1353
Size: 1521 772 3423
Fragments arranged by size:
3423 1521 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2602 2723 3779 4376 5163 5431
Size: 393 783 187 121 1056 597 787 268
Cuts at: 5431 1239
Size: 1524
Fragments arranged by size:
1524 1056 787 783 597 393 268 187
121
EcoO109I rG'GnC_Cy
Cuts at: 2140 2573 2140
Size: 433 5283
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 4863 1678
Size: 3185 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 2948 2980 4357
Size: 32 135 32 656 32 688 32 1377
Cuts at: 4357 4389 4431 4463 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1377 688 656 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2584 4830 5014 1305
Size: 1279 2246 184 2007
Fragments arranged by size:
2246 2007 1279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2444 3674 4033 4192 518
Size: 648 1086 192 1230 359 159 2042
Fragments arranged by size:
2042 1230 1086 648 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2092 3132 3809 4957 5202 1606
Size: 460 26 1040 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 677 460 245 26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5716
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2140
Size: 5716
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2092 2092
Size: 5716
SacII CC_GC'GG
Cuts at: 2067 2067
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3820 4721 4983 820
Size: 197 926 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 2182 2829 2918 1517
Size: 665 647 89 4315
Fragments arranged by size:
4315 665 647 89
StyI C'CwwG_G
Cuts at: 513 1065 2393 2919 3155 513
Size: 552 1328 526 236 3074
Fragments arranged by size:
3074 1328 552 526 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2371 3743 3760 3913 4098 4543
Size: 1255 446 345 1372 17 153 185 445
Cuts at: 4543 325
Size: 1498
Fragments arranged by size:
1498 1372 1255 446 445 345 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2261 2625
Size: 274 80 33 51 1443 147 137 364
Cuts at: 2625 3962 5674 96
Size: 1337 1712 138
Fragments arranged by size:
1712 1443 1337 364 274 147 138 137
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2847 2879 5676 5708 2847
Size: 32 2797 32 2855
Fragments arranged by size:
2855 2797 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 2770 3000 3845 2770
Size: 230 845 4641
Fragments arranged by size:
4641 845 230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2950 3524 276
Size: 53 83 186 936 1416 574 2468
Fragments arranged by size:
2468 1416 936 574 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI