Restriction map for
pFN21AB6477
(Circular) (Six-base) MAPSORT of: pFN21AB6477.seq Check: 2149 from: 1 to: 5683
pFN21AB6477 5683 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 3026 1937
Size: 849 240 4594
Fragments arranged by size:
4594 849 240
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 3746 5130 5398 1632
Size: 767 1347 1384 268 1917
Fragments arranged by size:
1917 1384 1347 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 2123 4412 2123
Size: 2289 3394
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2080 2377 2563 3426 4998 2080
Size: 297 186 863 1572 2765
Fragments arranged by size:
2765 1572 863 297 186
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2231 2243 2460 2992 3218 3370 5479 1493
Size: 738 12 217 532 226 152 2109 1697
Fragments arranged by size:
2109 1697 738 532 226 217 152 12
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 1870
Size: 73 1065 4545
Fragments arranged by size:
4545 1065 73
BamHI G'GATC_C
Cuts at: 2509 3013 3380 2509
Size: 504 367 4812
Fragments arranged by size:
4812 504 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2136 2730 3004 4459 618
Size: 325 217 627 349 594 274 1455 1842
Fragments arranged by size:
1842 1455 627 594 349 325 274 217
BanII G_rGCy'C
Cuts at: 1391 2148 2408 3002 1391
Size: 757 260 594 4072
Fragments arranged by size:
4072 757 594 260
BbeI G_GCGC'C
Cuts at: 2734 2734
Size: 5683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 4294 136
Size: 107 122 71 631 605 616 2006 1525
Fragments arranged by size:
2006 1525 631 616 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 2051 2941 3100 2051
Size: 890 159 4634
Fragments arranged by size:
4634 890 159
Bme1580I G_kGCm'C
Cuts at: 1790 3744 4900 1790
Size: 1954 1156 2573
Fragments arranged by size:
2573 1954 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2076 2076
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2768 4343 1239
Size: 812 717 1575 2579
Fragments arranged by size:
2579 1575 812 717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2731 3490 3872 4507
Size: 53 83 186 936 1198 759 382 635
Cuts at: 4507 275
Size: 1451
Fragments arranged by size:
1451 1198 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5458
Fragments arranged by size:
5458 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2486 2493 1186
Size: 1297 3 7 4376
Fragments arranged by size:
4376 1297 7 3
BseYI C'CCAG_C
Cuts at: 2960 4886 5382 2960
Size: 1926 496 3261
Fragments arranged by size:
3261 1926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 1163
Size: 573 357 4753
Fragments arranged by size:
4753 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 3744 3829 4900 2148
Size: 1596 85 1071 2931
Fragments arranged by size:
2931 1596 1071 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
BsmBI CGTCTCn'nnnn_
Cuts at: 2131 2131
Size: 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2408 3002 3744 3829 4900 1391
Size: 399 358 260 594 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 399 358 260 85
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 844
Size: 2194 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 2856 3743 4755 2856
Size: 887 1012 3784
Fragments arranged by size:
3784 1012 887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2071 794
Size: 1277 4406
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2509 2560 3013 3380 3765 3782 5234 5320
Size: 537 51 453 367 385 17 1452 86
Cuts at: 5320 5332 5678 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 537 453 385 367 346 86
51 17 12
BstZ17I GTA'TAC
Cuts at: 2787 2787
Size: 5683
Bsu36I CC'TnA_GG
Cuts at: 2505 2505
Size: 5683
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2915 3199 3993 4013 2915
Size: 284 794 20 4585
Fragments arranged by size:
4585 794 284 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648
Size: 54 1425 50 275 18 1300 889 1629
Cuts at: 5648 8
Size: 43
Fragments arranged by size:
1629 1425 1300 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 3613 1353
Size: 1068 1192 3423
Fragments arranged by size:
3423 1192 1068
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2399 2768 3746 4343 5130 5398
Size: 393 419 348 369 978 597 787 268
Cuts at: 5398 1239
Size: 1524
Fragments arranged by size:
1524 978 787 597 419 393 369 348
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2517 2517
Size: 5683
EcoO109I rG'GnC_Cy
Cuts at: 2117 2225 2522 2965 2117
Size: 108 297 443 4835
Fragments arranged by size:
4835 443 297 108
EcoRI G'AATT_C
Cuts at: 2231 2992 3370 2231
Size: 761 378 4544
Fragments arranged by size:
4544 761 378
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 2030 2734 4830 1678
Size: 352 704 2096 2531
Fragments arranged by size:
2531 2096 704 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 2420 2452 2795
Size: 32 135 32 537 32 279 32 343
Cuts at: 2795 2827 4324 4356 4398 4430 1373
Size: 32 1497 32 42 32 2626
Fragments arranged by size:
2626 1497 537 343 279 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2171 2550 2849 748
Size: 1423 379 299 3582
Fragments arranged by size:
3582 1423 379 299
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KasI G'GCGC_C
Cuts at: 2730 2730
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2620 2793 4797 4981 1305
Size: 1315 173 2004 184 2007
Fragments arranged by size:
2007 2004 1315 184 173
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2377 2672 2764 3099 3776 4924 5169 1606
Size: 771 295 92 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 677 335 295 245 92
NarI GG'CG_CC
Cuts at: 2731 2731
Size: 5683
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5131
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2706 3041 4586 1132
Size: 97 1477 335 1545 2229
Fragments arranged by size:
2229 1545 1477 335 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2117 2225 2522 2965 2117
Size: 108 297 443 4835
Fragments arranged by size:
4835 443 297 108
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2521 3035 830
Size: 1691 514 3478
Fragments arranged by size:
3478 1691 514
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2377 2672 2764 2377
Size: 295 92 5296
Fragments arranged by size:
5296 295 92
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SanDI GG'GwC_CC
Cuts at: 2117 2117
Size: 5683
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2517 3031 4166 4847 5038 826
Size: 219 1472 514 1135 681 191 1471
Fragments arranged by size:
1472 1471 1135 681 514 219 191
SfoI GGC'GCC
Cuts at: 2732 2732
Size: 5683
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 3122 513
Size: 552 2057 3074
Fragments arranged by size:
3074 2057 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3710 3727 3880 4065 4510 325
Size: 1255 2130 17 153 185 445 1498
Fragments arranged by size:
2130 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 2080 1316
Size: 764 4919
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstXI DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI
SrfI SwaI XcmI