Restriction map for
pFN21AB6466
(Circular) (Six-base) MAPSORT of: pFN21AB6466.seq Check: 5613 from: 1 to: 5761
pFN21AB6466 5761 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2377 3082 2377
Size: 705 5056
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 2421 2625 3074 3824 5208 5476 1632
Size: 566 223 204 449 750 1384 268 1917
Fragments arranged by size:
1917 1384 750 566 449 268 223 204
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 2817 4660 5637 1203
Size: 1614 1843 977 1327
Fragments arranged by size:
1843 1614 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2836 3504 5076 2836
Size: 668 1572 3521
Fragments arranged by size:
3521 1572 668
ApaI G_GGCC'C
Cuts at: 2090 2114 2090
Size: 24 5737
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2083 2113 2715 2973 3086 1870
Size: 73 140 30 602 258 113 4545
Fragments arranged by size:
4545 602 258 140 113 73 30
AvrII C'CTAG_G
Cuts at: 2881 2881
Size: 5761
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2377 3082 4537 618
Size: 325 217 627 590 705 1455 1842
Fragments arranged by size:
1842 1455 705 627 590 325 217
BanII G_rGCy'C
Cuts at: 1391 2090 2114 2183 2576 3000 3080 1391
Size: 699 24 69 393 424 80 4072
Fragments arranged by size:
4072 699 424 393 80 69 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2893 928
Size: 971 994 3796
Fragments arranged by size:
3796 994 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2519 2553 3952 3986 1903
Size: 34 582 34 1399 34 3678
Fragments arranged by size:
3678 1399 582 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 2892 1548
Size: 1344 4417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2163 5756 2163
Size: 3593 2168
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2114 3822 4978 1790
Size: 300 24 1708 1156 2573
Fragments arranged by size:
2573 1708 1156 300 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2560 4421 1239
Size: 1321 1861 2579
Fragments arranged by size:
2579 1861 1321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2502 3886 4751 5049 2502
Size: 1384 865 298 3214
Fragments arranged by size:
3214 1384 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 2766 3568 3950
Size: 53 83 186 936 825 408 802 382
Cuts at: 3950 4585 275
Size: 635 1451
Fragments arranged by size:
1451 936 825 802 635 408 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 2987 3017 1178
Size: 30 165 30 1368 30 186 30 3922
Fragments arranged by size:
3922 1368 186 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3008 1186
Size: 1822 3939
BseYI C'CCAG_C
Cuts at: 2306 2987 4964 5460 2306
Size: 681 1977 496 2607
Fragments arranged by size:
2607 1977 681 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2915 1163
Size: 573 1179 4009
Fragments arranged by size:
4009 1179 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2576 3000 3822 3907 4978 2576
Size: 424 822 85 1071 3359
Fragments arranged by size:
3359 1071 822 424 85
BsmI GAATG_Cn'
Cuts at: 2205 3272 3365 2205
Size: 1067 93 4601
Fragments arranged by size:
4601 1067 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2114 2183 2576 3000 3080 3822
Size: 399 300 24 69 393 424 80 742
Cuts at: 3822 3907 4978 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 393 300 85
80 69 24
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 3648 1394
Size: 429 281 1544 3507
Fragments arranged by size:
3507 1544 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2163 3091 3458 3843 3860 5312 5398 5410
Size: 191 928 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 928 385 367 346 191 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2740 2805 3047 513
Size: 552 939 736 65 242 3227
Fragments arranged by size:
3227 939 736 552 242 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2443 2950 2965 487
Size: 683 56 1217 507 15 3283
Fragments arranged by size:
3283 1217 683 507 56 15
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 2673 1554
Size: 1119 4642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726
Size: 54 1425 50 275 18 1378 889 1629
Cuts at: 5726 8
Size: 43
Fragments arranged by size:
1629 1425 1378 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2286 2576 3691 1353
Size: 861 72 290 1115 3423
Fragments arranged by size:
3423 1115 861 290 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2574 2998 2574
Size: 424 5337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2421 2560 2625 3074 3824 4421
Size: 393 566 223 139 65 449 750 597
Cuts at: 4421 5208 5476 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 750 597 566 449 393 268
223 139 65
EcoO109I rG'GnC_Cy
Cuts at: 2110 2121 2369 2500 2746 2797 2110
Size: 11 248 131 246 51 5074
Fragments arranged by size:
5074 248 246 131 51 11
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2328 4908 1678
Size: 650 2580 2531
Fragments arranged by size:
2580 2531 650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 4402 4434 4476
Size: 32 135 32 1013 32 1785 32 42
Cuts at: 4476 4508 1373
Size: 32 2626
Fragments arranged by size:
2626 1785 1013 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 2381 3086 2381
Size: 705 5056
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3939
Fragments arranged by size:
3939 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3719 4078 4237 518
Size: 648 2553 359 159 2042
Fragments arranged by size:
2553 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3177 3854 5002 5247 1606
Size: 1571 677 1148 245 2120
Fragments arranged by size:
2120 1571 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2821 3062 3119 4664 1132
Size: 97 1592 241 57 1545 2229
Fragments arranged by size:
2229 1592 1545 241 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 2817 4660 2817
Size: 1843 3918
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PfoI T'CCnGG_A
Cuts at: 2576 2576
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2177 2209 3830 3862 5343 5375 2177
Size: 32 1621 32 1481 32 2563
Fragments arranged by size:
2563 1621 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2369 2746 2797 2369
Size: 377 51 5333
Fragments arranged by size:
5333 377 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PspOMI G'GGCC_C
Cuts at: 2086 2110 2086
Size: 24 5737
PstI C_TGCA'G
Cuts at: 830 2601 3113 830
Size: 1771 512 3478
Fragments arranged by size:
3478 1771 512
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
SacI G_AGCT'C
Cuts at: 2576 3000 2576
Size: 424 5337
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SapI GCTCTTCn'nnn_
Cuts at: 2576 2576
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2278 2597 3109 4244 4925 5116 826
Size: 219 1233 319 512 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 681 512 319 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 2085 2115 2717 3088 1872
Size: 213 30 602 371 4545
Fragments arranged by size:
4545 602 371 213 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2517 3865 4766 5028 820
Size: 197 926 574 1348 901 262 1553
Fragments arranged by size:
1553 1348 926 901 574 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 2881 3200 513
Size: 552 939 877 319 3074
Fragments arranged by size:
3074 939 877 552 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 3805 3958 4143 4588 325
Size: 1255 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2820 4007 5719
Size: 274 80 33 51 1443 843 1187 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1712 1443 1187 843 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2083 2113 2715 3086 1870
Size: 213 30 602 371 4545
Fragments arranged by size:
4545 602 371 213 30
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI