Restriction map for
pFN21AB6464
(Circular) (Six-base) MAPSORT of: pFN21AB6464.seq Check: 7703 from: 1 to: 5761
pFN21AB6464 5761 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2168 2168
Size: 5761
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2288 3571 278
Size: 53 83 186 936 752 1283 2468
Fragments arranged by size:
2468 1283 936 752 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3060 3888 4261 1563
Size: 1497 828 373 3063
Fragments arranged by size:
3063 1497 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2482 2691 3824 5208 5476 1632
Size: 560 290 209 1133 1384 268 1917
Fragments arranged by size:
1917 1384 1133 560 290 268 209
AfeI AGC'GCT
Cuts at: 2059 2059
Size: 5761
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 2207 1166
Size: 1041 4720
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2225 1184
Size: 1041 4720
AlwNI CAG_nnn'CTG
Cuts at: 2312 3504 5076 2312
Size: 1192 1572 2997
Fragments arranged by size:
2997 1572 1192
ApaI G_GGCC'C
Cuts at: 2023 2888 2023
Size: 865 4896
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2414 3070 3296 3448 5557 1493
Size: 921 656 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 656 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2605 3086 1870
Size: 73 72 590 481 4545
Fragments arranged by size:
4545 590 481 73 72
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2464 3082 4537 618
Size: 325 217 627 677 618 1455 1842
Fragments arranged by size:
1842 1455 677 627 618 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2595 2888 3080 1391
Size: 632 572 293 192 4072
Fragments arranged by size:
4072 632 572 293 192
BbeI G_GCGC'C
Cuts at: 2468 2468
Size: 5761
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BbvCI CC'TCA_GC
Cuts at: 2474 2474
Size: 5761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2703 4372
Size: 107 122 71 631 605 376 655 1669
Cuts at: 4372 136
Size: 1525
Fragments arranged by size:
1669 1525 655 631 605 376 122 107
71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2036 3178 2036
Size: 1142 4619
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2888 3822 4978 1790
Size: 233 865 934 1156 2573
Fragments arranged by size:
2573 1156 934 865 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2499 2499
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 4421 1239
Size: 1071 2111 2579
Fragments arranged by size:
2579 2111 1071
Bpu10I CC'TnA_GC
Cuts at: 2474 2474
Size: 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2285 2465 3568 3950
Size: 53 83 186 936 752 180 1103 382
Cuts at: 3950 4585 275
Size: 635 1451
Fragments arranged by size:
1451 1103 936 752 635 382 186 180
83 53
BsaWI w'CCGG_w
Cuts at: 2613 4194 4592 4866 5013 5424 2613
Size: 1581 398 274 147 411 2950
Fragments arranged by size:
2950 1581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2294 3053 1186
Size: 837 271 759 3894
Fragments arranged by size:
3894 837 759 271
BseYI C'CCAG_C
Cuts at: 2212 2701 4964 5460 2212
Size: 489 2263 496 2513
Fragments arranged by size:
2513 2263 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 3022 1163
Size: 573 381 905 3902
Fragments arranged by size:
3902 905 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2715 3211 3972 5000 1540
Size: 275 186 714 496 761 1028 2301
Fragments arranged by size:
2301 1028 761 714 496 275 186
BsiHKAI G_wGCw'C
Cuts at: 2204 3822 3907 4978 2204
Size: 1618 85 1071 2987
Fragments arranged by size:
2987 1618 1071 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
BsmBI CGTCTCn'nnnn_
Cuts at: 2276 2276
Size: 5761
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2204 2595 2888 3080 3822 3907
Size: 399 233 181 391 293 192 742 85
Cuts at: 3907 4978 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 293 233 192
181 85
BspEI T'CCGG_A
Cuts at: 2613 5424 2613
Size: 2811 2950
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2168 2730 2852 3116 844
Size: 1324 562 122 264 3489
Fragments arranged by size:
3489 1324 562 264 122
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2714 4405 4592 1700
Size: 110 904 1691 187 2869
Fragments arranged by size:
2869 1691 904 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2921 794
Size: 2127 3634
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2121 3091 3458 3843 3860 5312 5398 5410
Size: 149 970 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 970 385 367 346 149 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2529 2529
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2355 2547 2763 513
Size: 552 1290 192 216 3511
Fragments arranged by size:
3511 1290 552 216 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2712 3208
Size: 54 1425 50 275 18 315 567 496
Cuts at: 3208 4097 5726 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 567 496 315 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2712 3208 1537
Size: 275 900 496 4090
Fragments arranged by size:
4090 900 496 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 2951 3691 1353
Size: 1278 320 740 3423
Fragments arranged by size:
3423 1278 740 320
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 2278 4348 4722 4868 387
Size: 1064 138 660 29 2070 374 146 1280
Fragments arranged by size:
2070 1280 1064 660 374 146 138 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2310 2482 2691 3824 4421 5208
Size: 393 560 118 172 209 1133 597 787
Cuts at: 5208 5476 1239
Size: 268 1524
Fragments arranged by size:
1524 1133 787 597 560 393 268 209
172 118
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 2884 2885 2019
Size: 1 864 1 4895
Fragments arranged by size:
4895 864 1 1
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2043 2061 2226 2468 4908 1678
Size: 365 18 165 242 2440 2531
Fragments arranged by size:
2531 2440 365 242 165 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2270 2271 2302 2630
Size: 32 135 32 667 31 1 31 328
Cuts at: 2630 2662 2886 2918 4402 4434 4476 4508 1373
Size: 32 224 32 1484 32 42 32 2626
Fragments arranged by size:
2626 1484 667 328 224 135 42 32
32 32 32 32 32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KasI G'GCGC_C
Cuts at: 2464 2464
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3081 4875 5059 1305
Size: 1776 1794 184 2007
Fragments arranged by size:
2007 1794 1776 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 10
Size: 54 1425 343 315 3624
Fragments arranged by size:
3624 1425 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2207 2354 2729 3719 4078 4237
Size: 648 927 114 147 375 990 359 159
Cuts at: 4237 518
Size: 2042
Fragments arranged by size:
2042 990 927 648 375 359 159 147
114
MspA1I CmG'CkG
Cuts at: 1606 2473 2701 3177 3854 5002 5247 1606
Size: 867 228 476 677 1148 245 2120
Fragments arranged by size:
2120 1148 867 677 476 245 228
NarI GG'CG_CC
Cuts at: 2465 2465
Size: 5761
NcoI C'CATG_G
Cuts at: 513 1065 2355 2547 2763 513
Size: 552 1290 192 216 3511
Fragments arranged by size:
3511 1290 552 216 192
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2512 2573 1348
Size: 1164 61 4536
Fragments arranged by size:
4536 1164 61
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2518 1108
Size: 1410 4351
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PspOMI G'GGCC_C
Cuts at: 2019 2884 2019
Size: 865 4896
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 5729
PstI C_TGCA'G
Cuts at: 830 2493 2841 3113 830
Size: 1663 348 272 3478
Fragments arranged by size:
3478 1663 348 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2473 2701 2473
Size: 228 5533
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SbfI CC_TGCA'GG
Cuts at: 2493 3113 2493
Size: 620 5141
ScaI AGT'ACT
Cuts at: 3011 4009 5721 3011
Size: 998 1712 3051
Fragments arranged by size:
3051 1712 998
SfcI C'TryA_G
Cuts at: 826 1045 2226 2489 2837 3109 4244 4925 5116
Size: 219 1181 263 348 272 1135 681 191
Cuts at: 5116 826
Size: 1471
Fragments arranged by size:
1471 1181 1135 681 348 272 263 219
191
SfoI GGC'GCC
Cuts at: 2466 2466
Size: 5761
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2355 2547 2763 3200 513
Size: 552 1290 192 216 437 3074
Fragments arranged by size:
3074 1290 552 437 216 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2571 3788 3805 3958 4143 4588 325
Size: 1255 991 1217 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1217 991 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3009 4007 5719
Size: 274 80 33 51 1443 1032 998 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1712 1443 1032 998 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2545 2545
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 2981 3890 2981
Size: 909 4852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2286 3569 276
Size: 53 83 186 936 752 1283 2468
Fragments arranged by size:
2468 1283 936 752 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI