Restriction map for
pFN21AB6462
(Circular) (Six-base) MAPSORT of: pFN21AB6462.seq Check: 6379 from: 1 to: 5761
pFN21AB6462 5761 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2494 3104 1937
Size: 557 610 4594
Fragments arranged by size:
4594 610 557
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2417 2844 3824 5208 5476 1632
Size: 465 320 427 980 1384 268 1917
Fragments arranged by size:
1917 1384 980 465 427 320 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2701 3504 5076 2701
Size: 803 1572 3386
Fragments arranged by size:
3386 1572 803
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2300 2463 2570 3039 3070 3296 3448 5557
Size: 807 163 107 469 31 226 152 2109
Cuts at: 5557 1493
Size: 1697
Fragments arranged by size:
2109 1697 807 469 226 163 152 107
31
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3082 4537 618
Size: 325 217 627 1295 1455 1842
Fragments arranged by size:
1842 1455 1295 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2896 3080 1391
Size: 1505 184 4072
Fragments arranged by size:
4072 1505 184
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 2214 1548
Size: 666 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3012 4372 136
Size: 107 122 71 631 605 1340 1360 1525
Fragments arranged by size:
1525 1360 1340 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2452 5756 2452
Size: 3304 2457
BlpI GC'TnA_GC
Cuts at: 2202 2207 2667 3178 2202
Size: 5 460 511 4785
Fragments arranged by size:
4785 511 460 5
Bme1580I G_kGCm'C
Cuts at: 1790 3822 4978 1790
Size: 2032 1156 2573
Fragments arranged by size:
2573 2032 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2222
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2693 2813 2826 4421 1239
Size: 1454 120 13 1595 2579
Fragments arranged by size:
2579 1595 1454 120 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 3886 4751 5049 2006
Size: 1880 865 298 2718
Fragments arranged by size:
2718 1880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2837 4424 882
Size: 617 1338 1587 2219
Fragments arranged by size:
2219 1587 1338 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2497 3568 3950 4585
Size: 53 83 186 936 964 1071 382 635
Cuts at: 4585 275
Size: 1451
Fragments arranged by size:
1451 1071 964 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2539 4194 4592 4866 5013 5424 2539
Size: 1655 398 274 147 411 2876
Fragments arranged by size:
2876 1655 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2833 2863 1178
Size: 30 165 30 1430 30 4076
Fragments arranged by size:
4076 1430 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 1186
Size: 899 4862
BseYI C'CCAG_C
Cuts at: 2338 4964 5460 2338
Size: 2626 496 2639
Fragments arranged by size:
2639 2626 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2637 1163
Size: 573 901 4287
Fragments arranged by size:
4287 901 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2896 3822 3907 4978 2896
Size: 926 85 1071 3679
Fragments arranged by size:
3679 1071 926 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2896 3080 3822 3907 4978 1391
Size: 399 1106 184 742 85 1071 2174
Fragments arranged by size:
2174 1106 1071 742 399 184 85
BspEI T'CCGG_A
Cuts at: 2539 5424 2539
Size: 2885 2876
BspHI T'CATG_A
Cuts at: 2443 3650 5380 2443
Size: 1207 1730 2824
Fragments arranged by size:
2824 1730 1207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 4405 4592 1700
Size: 110 205 2390 187 2869
Fragments arranged by size:
2869 2390 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2452 2697 3091 3458 3843 3860 5312 5398
Size: 480 245 394 367 385 17 1452 86
Cuts at: 5398 5410 5756 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 480 394 385 367 346 245
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2265 2265
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2236 513
Size: 552 1171 4038
Fragments arranged by size:
4038 1171 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 2938 3065 2938
Size: 127 5634
DraIII CAC_nnn'GTG
Cuts at: 2348 2348
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2239 3004
Size: 54 1425 50 275 18 183 226 765
Cuts at: 3004 3208 4097 5726 8
Size: 204 889 1629 43
Fragments arranged by size:
1629 1425 889 765 275 226 204 183
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2302 2436 2541 3691 1353
Size: 949 134 105 1150 3423
Fragments arranged by size:
3423 1150 949 134 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2894 2894
Size: 5761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2417 2693 2813 2826 2844 3824
Size: 393 465 320 276 120 13 18 980
Cuts at: 3824 4421 5208 5476 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 980 787 597 465 393 320 276
268 120 18 13
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
EcoRV GAT'ATC
Cuts at: 2950 2950
Size: 5761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2760 2434
Size: 326 5435
FseI GG_CCGG'CC
Cuts at: 2019 2019
Size: 5761
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2279 4908 1678
Size: 601 2629 2531
Fragments arranged by size:
2629 2531 601
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373
Size: 32 135 32 2830 32 42 32 2626
Fragments arranged by size:
2830 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2495 3105 3351 669
Size: 988 281 557 610 246 3079
Fragments arranged by size:
3079 988 610 557 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 2166 3360 2166
Size: 1194 4567
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2047 2358 2466 2825 4875 5059 1305
Size: 742 311 108 359 2050 184 2007
Fragments arranged by size:
2050 2007 742 359 311 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2241 3006 10
Size: 54 1425 343 409 765 2765
Fragments arranged by size:
2765 1425 765 409 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2405 3719 4078 4237 518
Size: 648 843 396 1314 359 159 2042
Fragments arranged by size:
2042 1314 843 648 396 359 159
MspA1I CmG'CkG
Cuts at: 1606 2671 3177 3854 5002 5247 1606
Size: 1065 506 677 1148 245 2120
Fragments arranged by size:
2120 1148 1065 677 506 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 5761
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5209
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2608 3119 4664 1132
Size: 97 1379 511 1545 2229
Fragments arranged by size:
2229 1545 1379 511 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2345 1108
Size: 1237 4524
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 2514 3331 2514
Size: 817 4944
PstI C_TGCA'G
Cuts at: 830 2706 3113 830
Size: 1876 407 3478
Fragments arranged by size:
3478 1876 407
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2671 2671
Size: 5761
SacI G_AGCT'C
Cuts at: 2896 2896
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 2493 3103 1936
Size: 557 610 4594
Fragments arranged by size:
4594 610 557
SapI GCTCTTCn'nnn_
Cuts at: 2541 2541
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 2815 4009 5721 2815
Size: 1194 1712 2855
Fragments arranged by size:
2855 1712 1194
SfcI C'TryA_G
Cuts at: 826 1045 2702 3109 4244 4925 5116 826
Size: 219 1657 407 1135 681 191 1471
Fragments arranged by size:
1657 1471 1135 681 407 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 3865 4766 5028 820
Size: 197 926 78 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 3200 513
Size: 552 2135 3074
Fragments arranged by size:
3074 2135 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 3805 3958 4143 4588 325
Size: 1255 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2813 4007 5719
Size: 274 80 33 51 1443 836 1194 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1712 1443 1194 836 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2833 2865 5721 5753 2833
Size: 32 2856 32 2841
Fragments arranged by size:
2856 2841 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 2563 3097 2563
Size: 534 5227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3031 3031
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 2030 2175 2550 3890 2030
Size: 145 375 1340 3901
Fragments arranged by size:
3901 1340 375 145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI