Restriction map for
pFN21AB6461
(Circular) (Six-base) MAPSORT of: pFN21AB6461.seq Check: 3759 from: 1 to: 5668
pFN21AB6461 5668 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2452 2452
Size: 5668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 2708 3731 5115 5383 1632
Size: 759 317 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1023 759 317 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 2487 4567 5544 1203
Size: 1284 2080 977 1327
Fragments arranged by size:
2080 1327 1284 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 2229 1166
Size: 1063 4605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2278 2736 2927 3411 4983 2278
Size: 458 191 484 1572 2963
Fragments arranged by size:
2963 1572 484 458 191
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 2837 2989 4444 618
Size: 325 217 627 756 294 152 1455 1842
Fragments arranged by size:
1842 1455 756 627 325 294 217 152
BanII G_rGCy'C
Cuts at: 1391 2743 2754 2798 2987 1391
Size: 1352 11 44 189 4072
Fragments arranged by size:
4072 1352 189 44 11
BbeI G_GCGC'C
Cuts at: 2841 2841
Size: 5668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2655 4279 136
Size: 107 122 71 631 605 983 1624 1525
Fragments arranged by size:
1624 1525 983 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2359 5663 2359
Size: 3304 2364
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2495 2546 3729 4885 1790
Size: 705 51 1183 1156 2573
Fragments arranged by size:
2573 1183 1156 705 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2152 2248 2746
Size: 416 53 83 399 24 735 96 498
Cuts at: 2746 3399 4357 442
Size: 653 958 1753
Fragments arranged by size:
1753 958 735 653 498 416 399 96
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2727 4328 1239
Size: 1061 427 1601 2579
Fragments arranged by size:
2579 1601 1061 427
Bpu10I CC'TnA_GC
Cuts at: 2447 2447
Size: 5668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2184 3793 4658 4956 2184
Size: 1609 865 298 2896
Fragments arranged by size:
2896 1609 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2490 2722 3459 493
Size: 711 1286 232 737 2702
Fragments arranged by size:
2702 1286 737 711 232
BsaBI GATnn'nnATC
Cuts at: 2311 5662 2311
Size: 3351 2317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2814 2838 3475 3857
Size: 53 83 186 936 1281 24 637 382
Cuts at: 3857 4492 275
Size: 635 1451
Fragments arranged by size:
1451 1281 936 637 635 382 186 83
53 24
BsaWI w'CCGG_w
Cuts at: 2798 4101 4499 4773 4920 5331 2798
Size: 1303 398 274 147 411 3135
Fragments arranged by size:
3135 1303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2038 2068 2642 2672 1178
Size: 30 165 30 635 30 574 30 4174
Fragments arranged by size:
4174 635 574 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2447 2633 1186
Size: 862 399 186 4221
Fragments arranged by size:
4221 862 399 186
BseYI C'CCAG_C
Cuts at: 2083 2111 2833 4871 5367 2083
Size: 28 722 2038 496 2384
Fragments arranged by size:
2384 2038 722 496 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 2827 2935 3118 3879 4907
Size: 275 186 802 24 108 183 761 1028
Cuts at: 4907 1540
Size: 2301
Fragments arranged by size:
2301 1028 802 761 275 186 183 108
24
BsiHKAI G_wGCw'C
Cuts at: 3729 3814 4885 3729
Size: 85 1071 4512
Fragments arranged by size:
4512 1071 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
BsmBI CGTCTCn'nnnn_
Cuts at: 2200 2200
Size: 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2495 2546 2743 2754 2798 2987 3729
Size: 399 705 51 197 11 44 189 742
Cuts at: 3729 3814 4885 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 705 399 197 189 85
51 44 11
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 3023 844
Size: 1608 571 3489
Fragments arranged by size:
3489 1608 571
BsrBI CCG'CTC
Cuts at: 1394 1823 2102 3555 1394
Size: 429 279 1453 3507
Fragments arranged by size:
3507 1453 429 279
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2654 4312 4499 1700
Size: 110 844 1658 187 2869
Fragments arranged by size:
2869 1658 844 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2490 794
Size: 1696 3972
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 2092 2231 1184
Size: 908 139 4621
Fragments arranged by size:
4621 908 139
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2215
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2359 2998 3365 3750 3767 5219 5305 5317
Size: 387 639 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 639 387 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2227 2892 2901 513
Size: 552 939 223 665 9 3280
Fragments arranged by size:
3280 939 665 552 223 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2558 3184 3978 3998 2558
Size: 626 794 20 4228
Fragments arranged by size:
4228 794 626 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2859 2932 3115
Size: 54 1425 50 275 18 1029 73 183
Cuts at: 3115 4004 5633 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1029 889 275 183 73 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 3115 1537
Size: 275 1120 183 4090
Fragments arranged by size:
4090 1120 275 183
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 3598 1353
Size: 816 1429 3423
Fragments arranged by size:
3423 1429 816
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2391 2708 2727 3731 4328 5115
Size: 393 668 91 317 19 1004 597 787
Cuts at: 5115 5383 1239
Size: 268 1524
Fragments arranged by size:
1524 1004 787 668 597 393 317 268
91 19
EcoO109I rG'GnC_Cy
Cuts at: 2906 2906
Size: 5668
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2841 4815 1678
Size: 1163 1974 2531
Fragments arranged by size:
2531 1974 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 4309 4341 4383
Size: 32 135 32 514 32 2191 32 42
Cuts at: 4383 4415 1373
Size: 32 2626
Fragments arranged by size:
2626 2191 514 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2403 748
Size: 1655 4013
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KasI G'GCGC_C
Cuts at: 2837 2837
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2539 4782 4966 1305
Size: 1234 2243 184 2007
Fragments arranged by size:
2243 2007 1234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2861 10
Size: 54 1425 343 1029 2817
Fragments arranged by size:
2817 1425 1029 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2229 3626 3985 4144 518
Size: 648 1063 1397 359 159 2042
Fragments arranged by size:
2042 1397 1063 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2472 2596 2857 2894 3084 3761 4909
Size: 564 302 124 261 37 190 677 1148
Cuts at: 4909 5154 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 564 302 261 245 190
124 37
NarI GG'CG_CC
Cuts at: 2838 2838
Size: 5668
NcoI C'CATG_G
Cuts at: 513 1065 2004 2901 513
Size: 552 939 897 3280
Fragments arranged by size:
3280 939 897 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2645 2868 3026 4571 1132
Size: 97 1416 223 158 1545 2229
Fragments arranged by size:
2229 1545 1416 223 158 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2553 1108
Size: 1445 4223
PfoI T'CCnGG_A
Cuts at: 2333 2427 2660 2333
Size: 94 233 5341
Fragments arranged by size:
5341 233 94
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PmlI CAC'GTG
Cuts at: 2490 2722 2490
Size: 232 5436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2906 2906
Size: 5668
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2170 2596 2857 2170
Size: 426 261 4981
Fragments arranged by size:
4981 426 261
SacII CC_GC'GG
Cuts at: 2895 2895
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2728 3016 4151 4832 5023 826
Size: 219 1683 288 1135 681 191 1471
Fragments arranged by size:
1683 1471 1135 681 288 219 191
SfoI GGC'GCC
Cuts at: 2839 2839
Size: 5668
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2199 3772 4673 4935 820
Size: 197 926 256 1573 901 262 1553
Fragments arranged by size:
1573 1553 926 901 262 256 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 2645 3026 1132
Size: 1513 381 3774
Fragments arranged by size:
3774 1513 381
SspI AAT'ATT
Cuts at: 5 52 2021 3592 5
Size: 47 1969 1571 2081
Fragments arranged by size:
2081 1969 1571 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2004 2901 3107 513
Size: 552 939 897 206 3074
Fragments arranged by size:
3074 939 897 552 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2695 2833 3695 3712 3865 4050 4495
Size: 1255 1115 138 862 17 153 185 445
Cuts at: 4495 325
Size: 1498
Fragments arranged by size:
1498 1255 1115 862 445 185 153 138
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 5628 5660 2642
Size: 32 2954 32 2650
Fragments arranged by size:
2954 2650 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI