Restriction map for
pFN21AB6460
(Circular) (Six-base) MAPSORT of: pFN21AB6460.seq Check: 7896 from: 1 to: 5758
pFN21AB6460 5758 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 2773 3821 5205 5473 1632
Size: 896 245 1048 1384 268 1917
Fragments arranged by size:
1917 1384 1048 896 268 245
AfeI AGC'GCT
Cuts at: 2846 2846
Size: 5758
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaI G_GGCC'C
Cuts at: 2677 2677
Size: 5758
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2057 3067 3293 3445 5554 1493
Size: 564 1010 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 564 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2324 3083 1870
Size: 73 381 759 4545
Fragments arranged by size:
4545 759 381 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2606 3079 4534 618
Size: 325 217 627 819 473 1455 1842
Fragments arranged by size:
1842 1455 819 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2430 2677 3077 1391
Size: 774 265 247 400 4072
Fragments arranged by size:
4072 774 400 265 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2968 3650 4860 2968
Size: 682 1210 3866
Fragments arranged by size:
3866 1210 682
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2742 2833 5753 2742
Size: 91 2920 2747
Fragments arranged by size:
2920 2747 91
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2379 2677 3819 4975 1790
Size: 589 298 1142 1156 2573
Fragments arranged by size:
2573 1156 1142 589 298
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4418 1239
Size: 3179 2579
Bpu10I CC'TnA_GC
Cuts at: 2639 2639
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3883 4748 5046 2843
Size: 1040 865 298 3555
Fragments arranged by size:
3555 1040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 3022 4421 882
Size: 617 693 830 1399 2219
Fragments arranged by size:
2219 1399 830 693 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 3549 493
Size: 711 1170 1175 2702
Fragments arranged by size:
2702 1175 1170 711
BsaBI GATnn'nnATC
Cuts at: 2918 5752 2918
Size: 2834 2924
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5758
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 1163
Size: 573 726 4459
Fragments arranged by size:
4459 726 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
BsmBI CGTCTCn'nnnn_
Cuts at: 2764 2764
Size: 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2379 2430 2677 3077 3819 3904
Size: 399 375 214 51 247 400 742 85
Cuts at: 3904 4975 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 375 247 214
85 51
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2817 2818 3113 844
Size: 1973 1 295 3489
Fragments arranged by size:
3489 1973 295 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2108 3645 1394
Size: 429 285 1537 3507
Fragments arranged by size:
3507 1537 429 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2223 2488 2742 2833 3088 3455 3840 3857
Size: 251 265 254 91 255 367 385 17
Cuts at: 3857 5309 5395 5407 5753 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 265 255 254
251 91 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2661 513
Size: 552 939 657 3610
Fragments arranged by size:
3610 939 657 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 2962 3062 2962
Size: 100 5658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2700 3205 4094
Size: 54 1425 50 275 18 870 505 889
Cuts at: 4094 5723 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 870 505 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3688 1353
Size: 2335 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2422 4345 4719 4865 387
Size: 1064 138 833 1923 374 146 1280
Fragments arranged by size:
1923 1280 1064 833 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2773 3821 4418 5205 5473 1239
Size: 393 896 245 1048 597 787 268 1524
Fragments arranged by size:
1524 1048 896 787 597 393 268 245
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 2825
Size: 5758
EcoO109I rG'GnC_Cy
Cuts at: 2503 2674 2806 2966 2503
Size: 171 132 160 5295
Fragments arranged by size:
5295 171 160 132
EcoRI G'AATT_C
Cuts at: 2057 3067 3445 2057
Size: 1010 378 4370
Fragments arranged by size:
4370 1010 378
EcoRV GAT'ATC
Cuts at: 2878 2878
Size: 5758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2102 2791 2102
Size: 689 5069
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2848 4905 1678
Size: 1170 2057 2531
Fragments arranged by size:
2531 2057 1170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373
Size: 32 135 32 2827 32 42 32 2626
Fragments arranged by size:
2827 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 2872 3102 3348 669
Size: 988 281 364 570 230 246 3079
Fragments arranged by size:
3079 988 570 364 281 246 230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4872 5056 1305
Size: 3567 184 2007
Fragments arranged by size:
3567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2549 3716 4075 4234 518
Size: 648 1197 186 1167 359 159 2042
Fragments arranged by size:
2042 1197 1167 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2705 3174 3851 4999 5244 1606
Size: 1099 469 677 1148 245 2120
Fragments arranged by size:
2120 1148 1099 677 469 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2677 2969 1348
Size: 1329 292 4137
Fragments arranged by size:
4137 1329 292
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2806 2503
Size: 303 5455
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2673 2673
Size: 5758
PspXI vC'TCGA_Gb
Cuts at: 2324 2324
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2829 3110 830
Size: 1999 281 3478
Fragments arranged by size:
3478 1999 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 2829 3110 2829
Size: 281 5477
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2497 2825 3106 4241 4922 5113 826
Size: 219 1452 328 281 1135 681 191 1471
Fragments arranged by size:
1471 1452 1135 681 328 281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 2858 3862 4763 5025 820
Size: 197 926 381 534 1004 901 262 1553
Fragments arranged by size:
1553 1004 926 901 534 381 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2004 3197 513
Size: 552 939 1193 3074
Fragments arranged by size:
3074 1193 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4004 5716 96
Size: 274 80 33 51 1443 2027 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 2324 1943
Size: 381 5377
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 2057 2082 3887 2057
Size: 25 1805 3928
Fragments arranged by size:
3928 1805 25
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI