Restriction map for
pFN21AB6457
(Circular) (Six-base) MAPSORT of: pFN21AB6457.seq Check: 3781 from: 1 to: 5659
pFN21AB6457 5659 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 3002 1937
Size: 864 201 4594
Fragments arranged by size:
4594 864 201
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 2044 3786 4159 1563
Size: 481 1742 373 3063
Fragments arranged by size:
3063 1742 481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 3722 5106 5374 1632
Size: 413 1677 1384 268 1917
Fragments arranged by size:
1917 1677 1384 413 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 3402 4974 3402
Size: 1572 4087
ApaI G_GGCC'C
Cuts at: 2148 2776 2148
Size: 628 5031
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2695 2968 3194 3346 5455 1493
Size: 1202 273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 273 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2189 2222 2189
Size: 33 5626
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2980 4435 618
Size: 325 217 627 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2148 2759 2776 2978 1391
Size: 757 611 17 202 4072
Fragments arranged by size:
4072 757 611 202 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BbvCI CC'TCA_GC
Cuts at: 2701 2701
Size: 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2217 3551 4761 2217
Size: 1334 1210 3115
Fragments arranged by size:
3115 1334 1210
BclI T'GATC_A
Cuts at: 1548 2851 1548
Size: 1303 4356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2776 3720 4876 1790
Size: 358 628 944 1156 2573
Fragments arranged by size:
2573 1156 944 628 358
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4319 1239
Size: 3080 2579
Bpu10I CC'TnA_GC
Cuts at: 2701 2701
Size: 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 4322 882
Size: 617 504 2319 2219
Fragments arranged by size:
2319 2219 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 2879 5653 2879
Size: 2774 2885
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2184 1186
Size: 998 4661
BseYI C'CCAG_C
Cuts at: 2775 4862 5358 2775
Size: 2087 496 3076
Fragments arranged by size:
3076 2087 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2422 3720 3805 4876 2422
Size: 1298 85 1071 3205
Fragments arranged by size:
3205 1298 1071 85
BsiWI C'GTAC_G
Cuts at: 2682 2682
Size: 5659
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2422 2759 2776 2978 3720 3805
Size: 399 358 274 337 17 202 742 85
Cuts at: 3805 4876 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 337 274 202
85 17
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3014 844
Size: 2170 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 2539
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2074 2989 3356 3741 3758 5210 5296 5308
Size: 102 915 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 915 385 367 346 102 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2406 2406
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4168
Fragments arranged by size:
4168 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 3969 3989 3175
Size: 794 20 4845
Fragments arranged by size:
4845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2493 2963 2493
Size: 470 5189
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2670 3106 3995
Size: 54 1425 50 275 18 840 436 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 840 436 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2410 3589 1353
Size: 668 389 1179 3423
Fragments arranged by size:
3423 1179 668 389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2200 4246 4620 4766 387
Size: 1064 138 611 2046 374 146 1280
Fragments arranged by size:
2046 1280 1064 611 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 3722 4319 5106 5374 1239
Size: 393 413 1677 597 787 268 1524
Fragments arranged by size:
1677 1524 787 597 413 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2144 2145 2144
Size: 1 5658
EcoRI G'AATT_C
Cuts at: 2695 2968 3346 2695
Size: 273 378 5008
Fragments arranged by size:
5008 378 273
FspI TGC'GCA
Cuts at: 2097 4165 2097
Size: 2068 3591
HaeII r_GCGC'y
Cuts at: 1678 2770 4806 1678
Size: 1092 2036 2531
Fragments arranged by size:
2531 2036 1092
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373
Size: 32 135 32 2728 32 42 32 2626
Fragments arranged by size:
2728 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2554 748
Size: 1806 3853
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2102 2938 4773 4957 1305
Size: 797 836 1835 184 2007
Fragments arranged by size:
2007 1835 836 797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2091 3617 3976 4135 518
Size: 648 925 1526 359 159 2042
Fragments arranged by size:
2042 1526 925 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2867 3075 3752 4900 5145 1606
Size: 1261 208 677 1148 245 2120
Fragments arranged by size:
2120 1261 1148 677 245 208
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4168
Fragments arranged by size:
4168 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PspOMI G'GGCC_C
Cuts at: 2144 2772 2144
Size: 628 5031
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2867 2867
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SapI GCTCTTCn'nnn_
Cuts at: 2410 2410
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 2193 3907 5619 2193
Size: 1714 1712 2233
Fragments arranged by size:
2233 1714 1712
SfcI C'TryA_G
Cuts at: 826 1045 2733 3007 4142 4823 5014 826
Size: 219 1688 274 1135 681 191 1471
Fragments arranged by size:
1688 1471 1135 681 274 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2004 3098 513
Size: 552 939 1094 3074
Fragments arranged by size:
3074 1094 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3686 3703 3856 4041 4486 325
Size: 1255 2106 17 153 185 445 1498
Fragments arranged by size:
2106 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2191 2675 2869
Size: 274 80 33 51 1443 214 484 194
Cuts at: 2869 3905 5617 96
Size: 1036 1712 138
Fragments arranged by size:
1712 1443 1036 484 274 214 194 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2392 2995 2392
Size: 603 5056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2852 2852
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 2506 3788 2506
Size: 1282 4377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI