Restriction map for
pFN21AB6454
(Circular) (Six-base) MAPSORT of: pFN21AB6454.seq Check: 7775 from: 1 to: 5755
pFN21AB6454 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2572 2572
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 2439 3882 4255 1563
Size: 876 1443 373 3063
Fragments arranged by size:
3063 1443 876 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3029 3818 5202 5470 1632
Size: 1397 789 1384 268 1917
Fragments arranged by size:
1917 1397 1384 789 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2855 1184
Size: 1671 4084
AlwNI CAG_nnn'CTG
Cuts at: 3029 3498 5070 3029
Size: 469 1572 3714
Fragments arranged by size:
3714 1572 469
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2432 2631 3064 3290 3442 5551 1493
Size: 939 199 433 226 152 2109 1697
Fragments arranged by size:
2109 1697 939 433 226 199 152
AseI AT'TA_AT
Cuts at: 160 1032 2550 4309 160
Size: 872 1518 1759 1606
Fragments arranged by size:
1759 1606 1518 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2272 3074 1391
Size: 881 802 4072
Fragments arranged by size:
4072 881 802
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2630 928
Size: 971 731 4053
Fragments arranged by size:
4053 971 731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2034 2068 3946 3980 1903
Size: 34 97 34 1878 34 3678
Fragments arranged by size:
3678 1878 97 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2761 3172 2761
Size: 411 5344
Bme1580I G_kGCm'C
Cuts at: 1790 3816 4972 1790
Size: 2026 1156 2573
Fragments arranged by size:
2573 2026 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2356 2663 4415 1239
Size: 1055 62 307 1752 2579
Fragments arranged by size:
2579 1752 1055 307 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2778 3880 4745 5043 2778
Size: 1102 865 298 3490
Fragments arranged by size:
3490 1102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2331 3562 3944 4579
Size: 53 83 186 936 798 1231 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1451 1231 936 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2289 1186
Size: 988 115 4652
Fragments arranged by size:
4652 988 115
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2272 3074 3816 3901 4972 1391
Size: 399 482 802 742 85 1071 2174
Fragments arranged by size:
2174 1071 802 742 482 399 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 2887 3644 5374 2887
Size: 757 1730 3268
Fragments arranged by size:
3268 1730 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2572 3110 844
Size: 1728 538 3489
Fragments arranged by size:
3489 1728 538
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750
Size: 1113 367 385 17 1452 86 12 346
Cuts at: 5750 1972
Size: 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2484 3271 4065 4085 2484
Size: 787 794 20 4154
Fragments arranged by size:
4154 794 787 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720
Size: 54 1425 50 275 18 1372 889 1629
Cuts at: 5720 8
Size: 43
Fragments arranged by size:
1629 1425 1372 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 3048 3685 1353
Size: 776 919 637 3423
Fragments arranged by size:
3423 919 776 637
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2193 4342 4716 4862 387
Size: 1064 138 604 2149 374 146 1280
Fragments arranged by size:
2149 1280 1064 604 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2356 2663 3029 3818 4415 5202
Size: 393 662 62 307 366 789 597 787
Cuts at: 5202 5470 1239
Size: 268 1524
Fragments arranged by size:
1524 789 787 662 597 393 366 307
268 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2144 2144
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 3037 3037
Size: 5755
EcoRI G'AATT_C
Cuts at: 2432 3064 3442 2432
Size: 632 378 4745
Fragments arranged by size:
4745 632 378
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2257 4902 1678
Size: 579 2645 2531
Fragments arranged by size:
2645 2531 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2254 2274 2286
Size: 32 135 32 458 32 192 20 12
Cuts at: 2286 2306 2869 2901 4396 4428 4470 4502 1373
Size: 20 563 32 1495 32 42 32 2626
Fragments arranged by size:
2626 1495 563 458 192 135 42 32
32 32 32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 2443 3099 3345 669
Size: 988 281 397 108 656 246 3079
Fragments arranged by size:
3079 988 656 397 281 246 108
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 2443 3345 2443
Size: 902 4853
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2059 2239 4869 5053 1305
Size: 754 180 2630 184 2007
Fragments arranged by size:
2630 2007 754 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4072 4231 518
Size: 648 2547 359 159 2042
Fragments arranged by size:
2547 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3171 3848 4996 5241 1606
Size: 1565 677 1148 245 2120
Fragments arranged by size:
2120 1565 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2326 1348
Size: 978 4777
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2458 1108
Size: 1350 4405
PfoI T'CCnGG_A
Cuts at: 2600 2600
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3037 3037
Size: 5755
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2479 2844 3107 830
Size: 1649 365 263 3478
Fragments arranged by size:
3478 1649 365 263
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SanDI GG'GwC_CC
Cuts at: 3037 3037
Size: 5755
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2409 2475 2840 3103 4238 4919 5110
Size: 219 1364 66 365 263 1135 681 191
Cuts at: 5110 826
Size: 1471
Fragments arranged by size:
1471 1364 1135 681 365 263 219 191
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2793 3859 4760 5022 820
Size: 197 926 850 1066 901 262 1553
Fragments arranged by size:
1553 1066 926 901 850 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3013 3679 5
Size: 47 2961 666 2081
Fragments arranged by size:
2961 2081 666 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 2767 2911 3194 513
Size: 552 939 763 144 283 3074
Fragments arranged by size:
3074 939 763 552 283 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2465 3782 3799 3952 4137 4582 325
Size: 1255 885 1317 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 885 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2561 4001 5713
Size: 274 80 33 51 1443 584 1440 1712
Cuts at: 5713 96
Size: 138
Fragments arranged by size:
1712 1443 1440 584 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2073 2105 5715 5747 2073
Size: 32 3610 32 2081
Fragments arranged by size:
3610 2081 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2465 1316
Size: 1149 4606
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI