Restriction map for
pFN21AB6451
(Circular) (Six-base) MAPSORT of: pFN21AB6451.seq Check: 3954 from: 1 to: 5659
pFN21AB6451 5659 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2644 3002 1937
Size: 707 358 4594
Fragments arranged by size:
4594 707 358
Acc65I G'GTAC_C
Cuts at: 2251 2980 2251
Size: 729 4930
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2558 3722 5106 5374 1632
Size: 926 1164 1384 268 1917
Fragments arranged by size:
1917 1384 1164 926 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2171 2329 2825 3402 4974 2171
Size: 158 496 577 1572 2856
Fragments arranged by size:
2856 1572 577 496 158
ApaI G_GGCC'C
Cuts at: 2130 2130
Size: 5659
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2778 2799 2968 3194 3346 5455 1493
Size: 1285 21 169 226 152 2109 1697
Fragments arranged by size:
2109 1697 1285 226 169 152 21
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 2634 2888 2980 4435
Size: 325 217 627 464 383 254 92 1455
Cuts at: 4435 618
Size: 1842
Fragments arranged by size:
1842 1455 627 464 383 325 254 217
92
BanII G_rGCy'C
Cuts at: 1391 2130 2978 1391
Size: 739 848 4072
Fragments arranged by size:
4072 848 739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2088 3551 4761 2076
Size: 12 1463 1210 2974
Fragments arranged by size:
2974 1463 1210 12
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2181 4270 136
Size: 107 122 71 631 605 509 2089 1525
Fragments arranged by size:
2089 1525 631 605 509 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2130 3720 4876 1790
Size: 340 1590 1156 2573
Fragments arranged by size:
2573 1590 1156 340
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 2549 4319 1239
Size: 849 461 1770 2579
Fragments arranged by size:
2579 1770 849 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2509 3784 4649 4947 2509
Size: 1275 865 298 3221
Fragments arranged by size:
3221 1275 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2527 4322 882
Size: 617 1028 1795 2219
Fragments arranged by size:
2219 1795 1028 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 2585 5653 2585
Size: 3068 2591
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2943 2973 1178
Size: 30 165 30 1540 30 3864
Fragments arranged by size:
3864 1540 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2061 2586 1186
Size: 875 525 4259
Fragments arranged by size:
4259 875 525
BseYI C'CCAG_C
Cuts at: 2176 4862 5358 2176
Size: 2686 496 2477
Fragments arranged by size:
2686 2477 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2734 1163
Size: 573 998 4088
Fragments arranged by size:
4088 998 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3720 3805 4876 3720
Size: 85 1071 4503
Fragments arranged by size:
4503 1071 85
BsmI GAATG_Cn'
Cuts at: 2035 2090 3170 3263 2035
Size: 55 1080 93 4431
Fragments arranged by size:
4431 1080 93 55
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2978 3720 3805 4876 1391
Size: 399 340 848 742 85 1071 2174
Fragments arranged by size:
2174 1071 848 742 399 340 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3014 844
Size: 2170 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 4303 4490 1700
Size: 110 346 2147 187 2869
Fragments arranged by size:
2869 2147 346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654
Size: 1017 367 385 17 1452 86 12 346
Cuts at: 5654 1972
Size: 1977
Fragments arranged by size:
1977 1452 1017 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2255 513
Size: 552 939 251 3917
Fragments arranged by size:
3917 939 552 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 3969 3989 3175
Size: 794 20 4845
Fragments arranged by size:
4845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 3106 3995
Size: 54 1425 50 275 18 328 948 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 948 889 328 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3589 1353
Size: 2236 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2549 2558 3722 4319 5106 5374
Size: 393 456 461 9 1164 597 787 268
Cuts at: 5374 1239
Size: 1524
Fragments arranged by size:
1524 1164 787 597 461 456 393 268
9
EcoO109I rG'GnC_Cy
Cuts at: 2099 2127 2168 2099
Size: 28 41 5590
Fragments arranged by size:
5590 41 28
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 5659
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2270 4806 1678
Size: 592 2536 2531
Fragments arranged by size:
2536 2531 592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2734 2764 2766 4300
Size: 32 135 32 1160 2 30 2 1534
Cuts at: 4300 4332 4374 4406 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1534 1160 135 42 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2255 2984 2255
Size: 729 4930
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2218 4773 4957 1305
Size: 913 2555 184 2007
Fragments arranged by size:
2555 2007 913 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2542 3617 3976 4135 518
Size: 648 1376 1075 359 159 2042
Fragments arranged by size:
2042 1376 1075 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2432 2553 2718 3075 3752 4900 5145
Size: 574 252 121 165 357 677 1148 245
Cuts at: 5145 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 574 357 252 245 165
121
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 5659
NcoI C'CATG_G
Cuts at: 513 1065 2004 2255 513
Size: 552 939 251 3917
Fragments arranged by size:
3917 939 552 251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2123 1348
Size: 775 4884
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2790 1108
Size: 1682 3977
PfoI T'CCnGG_A
Cuts at: 2104 2104
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2099 2168 2099
Size: 69 5590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PspOMI G'GGCC_C
Cuts at: 2126 2126
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2248 3011 830
Size: 1418 763 3478
Fragments arranged by size:
3478 1418 763
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2180 2553 2718 2180
Size: 373 165 5121
Fragments arranged by size:
5121 373 165
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2244 3007 4142 4823 5014 826
Size: 219 1199 763 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 763 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2488 3763 4664 4926 820
Size: 197 926 545 1275 901 262 1553
Fragments arranged by size:
1553 1275 926 901 545 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 2306 3583 5
Size: 47 2254 1277 2081
Fragments arranged by size:
2254 2081 1277 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2004 2095 2255 3098 513
Size: 552 939 91 160 843 3074
Fragments arranged by size:
3074 939 843 552 160 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2652 3686 3703 3856 4041 4486 325
Size: 1255 1072 1034 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1072 1034 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2683 3905 5617
Size: 274 80 33 51 1443 706 1222 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1443 1222 706 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2474 2995 2474
Size: 521 5138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2597 2168
Size: 429 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI