Restriction map for
pFN21AB6450
(Circular) (Six-base) MAPSORT of: pFN21AB6450.seq Check: 3293 from: 1 to: 5752
pFN21AB6450 5752 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3815 5199 5467 1632
Size: 2183 1384 268 1917
Fragments arranged by size:
2183 1917 1384 268
AfeI AGC'GCT
Cuts at: 2700 2700
Size: 5752
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 2385 1166
Size: 1219 4533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2646 2678 2646
Size: 32 5720
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2474 2502 3061 3287 3439 5548 1493
Size: 981 28 559 226 152 2109 1697
Fragments arranged by size:
2109 1697 981 559 226 152 28
AseI AT'TA_AT
Cuts at: 160 1032 2405 4306 160
Size: 872 1373 1901 1606
Fragments arranged by size:
1901 1606 1373 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2673 2739 2745 3077 1870
Size: 73 730 66 6 332 4545
Fragments arranged by size:
4545 730 332 73 66 6
BamHI G'GATC_C
Cuts at: 2056 3082 3449 2056
Size: 1026 367 4359
Fragments arranged by size:
4359 1026 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2627 2752 2776 2995 3073
Size: 325 217 627 840 125 24 219 78
Cuts at: 3073 4528 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 840 627 325 219 217 125
78 24
BanII G_rGCy'C
Cuts at: 1391 2746 3071 1391
Size: 1355 325 4072
Fragments arranged by size:
4072 1355 325
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 928
Size: 971 240 4541
Fragments arranged by size:
4541 971 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2226 2762 3017 3644 4854 2226
Size: 536 255 627 1210 3124
Fragments arranged by size:
3124 1210 627 536 255
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BfrBI ATG'CAT
Cuts at: 2456 2456
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2750 4363 136
Size: 107 122 71 631 605 1078 1613 1525
Fragments arranged by size:
1613 1525 1078 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2755 2779 3813 4969 1790
Size: 965 24 1034 1156 2573
Fragments arranged by size:
2573 1156 1034 965 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2681
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2772 4412 1239
Size: 1533 1640 2579
Fragments arranged by size:
2579 1640 1533
Bpu10I CC'TnA_GC
Cuts at: 2028 2337 2028
Size: 309 5443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2404 3877 4742 5040 2404
Size: 1473 865 298 3116
Fragments arranged by size:
3116 1473 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2444 2522 3543 493
Size: 711 1240 78 1021 2702
Fragments arranged by size:
2702 1240 1021 711 78
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2628 2772 2775 1186
Size: 841 601 144 3 4163
Fragments arranged by size:
4163 841 601 144 3
BseYI C'CCAG_C
Cuts at: 4955 5451 4955
Size: 496 5256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2746 2755 2779 3071 3813 3898 4969
Size: 399 956 9 24 292 742 85 1071
Cuts at: 4969 1391
Size: 2174
Fragments arranged by size:
2174 1071 956 742 399 292 85 24
9
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2967 2967
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2056 3082 3449 3834 3851 5303 5389 5401
Size: 84 1026 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 84
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2620 2692 513
Size: 552 939 616 72 3573
Fragments arranged by size:
3573 939 616 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DraIII CAC_nnn'GTG
Cuts at: 2897 2897
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3199 4088 5717
Size: 54 1425 50 275 18 1369 889 1629
Cuts at: 5717 8
Size: 43
Fragments arranged by size:
1629 1425 1369 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2264 2351 2457 3682 1353
Size: 651 260 87 106 1225 3423
Fragments arranged by size:
3423 1225 651 260 106 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2772 3815 4412 5199 5467 1239
Size: 393 1140 1043 597 787 268 1524
Fragments arranged by size:
1524 1140 1043 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2269 2655 2269
Size: 386 5366
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2702 4899 1678
Size: 1024 2197 2531
Fragments arranged by size:
2531 2197 1024
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373
Size: 32 135 32 2821 32 42 32 2626
Fragments arranged by size:
2821 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2433 2799 748
Size: 1685 366 3701
Fragments arranged by size:
3701 1685 366
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2385 3039 3710 4069 4228 518
Size: 648 1219 654 671 359 159 2042
Fragments arranged by size:
2042 1219 671 654 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3168 3845 4993 5238 1606
Size: 1562 677 1148 245 2120
Fragments arranged by size:
2120 1562 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NsiI A_TGCA'T
Cuts at: 2458 2458
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2075 3110 4655 1132
Size: 97 846 1035 1545 2229
Fragments arranged by size:
2229 1545 1035 846 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2651 1108
Size: 1543 4209
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2556 2919 1613
Size: 943 363 4446
Fragments arranged by size:
4446 943 363
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspXI vC'TCGA_Gb
Cuts at: 2739 2739
Size: 5752
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2207 2239 2207
Size: 32 5720
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 2196 4000 5712 2196
Size: 1804 1712 2236
Fragments arranged by size:
2236 1804 1712
SfcI C'TryA_G
Cuts at: 826 1045 2276 2715 3100 4235 4916 5107 826
Size: 219 1231 439 385 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 681 439 385 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2747 3079 1872
Size: 875 332 4545
Fragments arranged by size:
4545 875 332
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2383 2739 3856 4757 5019 820
Size: 197 926 440 356 1117 901 262 1553
Fragments arranged by size:
1553 1117 926 901 440 356 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 2075 3110 1132
Size: 943 1035 3774
Fragments arranged by size:
3774 1035 943
SspI AAT'ATT
Cuts at: 5 52 2021 3676 5
Size: 47 1969 1655 2081
Fragments arranged by size:
2081 1969 1655 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 3191 513
Size: 552 939 1187 3074
Fragments arranged by size:
3074 1187 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2194 3998 5710
Size: 274 80 33 51 1443 217 1804 1712
Cuts at: 5710 96
Size: 138
Fragments arranged by size:
1804 1712 1443 274 217 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 2739 1943
Size: 796 4956
XmaI C'CCGG_G
Cuts at: 1870 2745 3077 1870
Size: 875 332 4545
Fragments arranged by size:
4545 875 332
XmnI GAAnn'nnTTC
Cuts at: 2106 3881 2106
Size: 1775 3977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI