Restriction map for
pFN21AB6449
(Circular) (Six-base) MAPSORT of: pFN21AB6449.seq Check: 3623 from: 1 to: 5656
pFN21AB6449 5656 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2563 2999 1937
Size: 626 436 4594
Fragments arranged by size:
4594 626 436
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2552 2819 3719 5103 5371 1632
Size: 452 468 267 900 1384 268 1917
Fragments arranged by size:
1917 1384 900 468 452 268 267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2556 2558 4555 5532 1203
Size: 1353 2 1997 977 1327
Fragments arranged by size:
1997 1353 1327 977 2
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 2701 2878 4385 2701
Size: 177 1507 3972
Fragments arranged by size:
3972 1507 177
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2809 2842 2947 3399 4971 2809
Size: 33 105 452 1572 3494
Fragments arranged by size:
3494 1572 452 105 33
ApaI G_GGCC'C
Cuts at: 2051 2051
Size: 5656
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2981 1870
Size: 73 222 816 4545
Fragments arranged by size:
4545 816 222 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2977 4432 618
Size: 325 217 627 392 798 1455 1842
Fragments arranged by size:
1842 1455 798 627 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2199 2421 2646 2975 1391
Size: 660 148 222 225 329 4072
Fragments arranged by size:
4072 660 329 225 222 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BbvCI CC'TCA_GC
Cuts at: 2385 2385
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3548 4758 2790
Size: 758 1210 3688
Fragments arranged by size:
3688 1210 758
BclI T'GATC_A
Cuts at: 1548 2361 1548
Size: 813 4843
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2051 3717 4873 1790
Size: 261 1666 1156 2573
Fragments arranged by size:
2573 1666 1156 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4316 1239
Size: 3077 2579
Bpu10I CC'TnA_GC
Cuts at: 2385 2415 2637 2385
Size: 30 222 5404
Fragments arranged by size:
5404 222 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2295 4319 882
Size: 617 796 2024 2219
Fragments arranged by size:
2219 2024 796 617
BsaAI yAC'GTr
Cuts at: 493 1204 2559 3447 493
Size: 711 1355 888 2702
Fragments arranged by size:
2702 1355 888 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 1178
Size: 30 165 30 1104 30 4297
Fragments arranged by size:
4297 1104 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2876 1186
Size: 952 738 3966
Fragments arranged by size:
3966 952 738
BseYI C'CCAG_C
Cuts at: 2809 4859 5355 2809
Size: 2050 496 3110
Fragments arranged by size:
3110 2050 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2488 1163
Size: 573 486 266 4331
Fragments arranged by size:
4331 573 486 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2089 3106 3867 4895 1540
Size: 275 186 18 70 1017 761 1028 2301
Fragments arranged by size:
2301 1028 1017 761 275 186 70 18
BsiHKAI G_wGCw'C
Cuts at: 2421 3717 3802 4873 2421
Size: 1296 85 1071 3204
Fragments arranged by size:
3204 1296 1071 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2199 2421 2646 2975 3717 3802
Size: 399 261 148 222 225 329 742 85
Cuts at: 3802 4873 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 329 261 225 222
148 85
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 2691 3011 844
Size: 1496 351 320 3489
Fragments arranged by size:
3489 1496 351 320
BsrBI CCG'CTC
Cuts at: 1394 1823 2074 3543 1394
Size: 429 251 1469 3507
Fragments arranged by size:
3507 1469 429 251
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2179 794
Size: 1385 4271
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2157 2986 3353 3738 3755 5207 5293 5305
Size: 185 829 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 829 385 367 346 185 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2040 2274 2581 513
Size: 552 975 234 307 3588
Fragments arranged by size:
3588 975 552 307 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2682 3172 3966 3986 2682
Size: 490 794 20 4352
Fragments arranged by size:
4352 794 490 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2334 2369 529
Size: 35 1770 35 3816
Fragments arranged by size:
3816 1770 35 35
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2244 2475
Size: 54 1425 50 275 18 186 228 231
Cuts at: 2475 2551 2653 3103 3992 5621 8
Size: 76 102 450 889 1629 43
Fragments arranged by size:
1629 1425 889 450 275 231 228 186
102 76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 3103 1537
Size: 275 204 1087 4090
Fragments arranged by size:
4090 1087 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 3586 1353
Size: 2233 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2150 2276 4243 4617 4763 387
Size: 1064 138 561 126 1967 374 146 1280
Fragments arranged by size:
1967 1280 1064 561 374 146 138 126
EcoICRI GAG'CTC
Cuts at: 2419 2419
Size: 5656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2552 2819 3719 4316 5103 5371
Size: 393 452 468 267 900 597 787 268
Cuts at: 5371 1239
Size: 1524
Fragments arranged by size:
1524 900 787 597 468 452 393 268
267
EcoO109I rG'GnC_Cy
Cuts at: 2047 2048 2047
Size: 1 5655
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 2026 4803 1678
Size: 348 2777 2531
Fragments arranged by size:
2777 2531 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2687 2719 2864
Size: 32 135 32 641 32 442 32 145
Cuts at: 2864 2896 4297 4329 4371 4403 1373
Size: 32 1401 32 42 32 2626
Fragments arranged by size:
2626 1401 641 442 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 3000 3246 669
Size: 988 281 796 266 246 3079
Fragments arranged by size:
3079 988 796 281 266 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 2477 2553 10
Size: 54 1425 343 414 231 76 3113
Fragments arranged by size:
3113 1425 414 343 231 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2279 3614 3973 4132 518
Size: 648 1113 1335 359 159 2042
Fragments arranged by size:
2042 1335 1113 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2809 2818 3072 3749 4897 5142 1606
Size: 494 709 9 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 709 677 494 254 245 9
NcoI C'CATG_G
Cuts at: 513 1065 2040 2274 2581 513
Size: 552 975 234 307 3588
Fragments arranged by size:
3588 975 552 307 234
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PmlI CAC'GTG
Cuts at: 2559 2559
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 3725 3757 5238 5270 2870
Size: 32 823 32 1481 32 3256
Fragments arranged by size:
3256 1481 823 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2047 2047
Size: 5656
PspXI vC'TCGA_Gb
Cuts at: 2165 2165
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2154 2337 3008 830
Size: 1324 183 671 3478
Fragments arranged by size:
3478 1324 671 183
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2809 2818 2809
Size: 9 5647
SacI G_AGCT'C
Cuts at: 2421 2421
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SexAI A'CCwGG_T
Cuts at: 2697 2697
Size: 5656
SfcI C'TryA_G
Cuts at: 826 1045 2150 2333 3004 4139 4820 5011 826
Size: 219 1105 183 671 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 681 671 219 191 183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 3760 4661 4923 820
Size: 197 926 222 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2040 2274 2581 2616 3095 513
Size: 552 975 234 307 35 479 3074
Fragments arranged by size:
3074 975 552 479 307 234 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2609 3683 3700 3853 4038 4483 325
Size: 1255 1029 1074 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1074 1029 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2507 2539 5616 5648 2507
Size: 32 3077 32 2515
Fragments arranged by size:
3077 2515 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2497 1316
Size: 1181 4475
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 2165 1943
Size: 222 5434
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 2832 3785 2832
Size: 953 4703
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI