Restriction map for
pFN21AB6447
(Circular) (Six-base) MAPSORT of: pFN21AB6447.seq Check: 3704 from: 1 to: 5431
pFN21AB6447 5431 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 2462 2624 3494 4878 5146 1632
Size: 650 180 162 870 1384 268 1917
Fragments arranged by size:
1917 1384 870 650 268 180 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 4330 5307 1203
Size: 3127 977 1327
Fragments arranged by size:
3127 1327 977
AgeI A'CCGG_T
Cuts at: 2676 4262 2676
Size: 1586 3845
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 2377 2428 3174 4746 2377
Size: 51 746 1572 3062
Fragments arranged by size:
3062 1572 746 51
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2740 2966 3118 5227 1493
Size: 1247 226 152 2109 1697
Fragments arranged by size:
2109 1697 1247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 1870
Size: 73 813 4545
Fragments arranged by size:
4545 813 73
BamHI G'GATC_C
Cuts at: 2316 2761 3128 2316
Size: 445 367 4619
Fragments arranged by size:
4619 445 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2552 2752 4207 618
Size: 325 217 627 765 200 1455 1842
Fragments arranged by size:
1842 1455 765 627 325 217 200
BanII G_rGCy'C
Cuts at: 1391 2750 1391
Size: 1359 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2543 928
Size: 971 138 506 3816
Fragments arranged by size:
3816 971 506 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3622 3656 1903
Size: 34 1685 34 3678
Fragments arranged by size:
3678 1685 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BfrBI ATG'CAT
Cuts at: 2167 2167
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4042 136
Size: 107 122 71 631 605 2370 1525
Fragments arranged by size:
2370 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2429 2848 2429
Size: 419 5012
Bme1580I G_kGCm'C
Cuts at: 1790 3492 4648 1790
Size: 1702 1156 2573
Fragments arranged by size:
2573 1702 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2249 3162 4120
Size: 416 53 83 399 24 832 913 958
Cuts at: 4120 442
Size: 1753
Fragments arranged by size:
1753 958 913 832 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2159 2435 2648 4091 1239
Size: 830 90 276 213 1443 2579
Fragments arranged by size:
2579 1443 830 276 213 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 3556 4421 4719 2339
Size: 1217 865 298 3051
Fragments arranged by size:
3051 1217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4094 882
Size: 617 2595 2219
Fragments arranged by size:
2595 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 3238 3620 4255
Size: 53 83 186 936 578 1127 382 635
Cuts at: 4255 275
Size: 1451
Fragments arranged by size:
1451 1127 936 635 578 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2676 2724 3864 4262 4536 4683 5094 2676
Size: 48 1140 398 274 147 411 3013
Fragments arranged by size:
3013 1140 411 398 274 147 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5206
Fragments arranged by size:
5206 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5431
BseYI C'CCAG_C
Cuts at: 2229 2505 4634 5130 2229
Size: 276 2129 496 2530
Fragments arranged by size:
2530 2129 496 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 1163
Size: 573 465 4393
Fragments arranged by size:
4393 573 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2681 2881 3642 4670 1540
Size: 275 186 680 200 761 1028 2301
Fragments arranged by size:
2301 1028 761 680 275 200 186
BsiHKAI G_wGCw'C
Cuts at: 2421 3492 3577 4648 2421
Size: 1071 85 1071 3204
Fragments arranged by size:
3204 1071 1071 85
BsmI GAATG_Cn'
Cuts at: 2942 3035 2942
Size: 93 5338
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2421 2750 3492 3577 4648 1391
Size: 399 631 329 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 631 399 329 85
BspEI T'CCGG_A
Cuts at: 2724 5094 2724
Size: 2370 3061
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 2786 844
Size: 1388 554 3489
Fragments arranged by size:
3489 1388 554
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 3318 1394
Size: 429 245 1250 3507
Fragments arranged by size:
3507 1250 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 3926 4100 65
Size: 1183 2678 174 1396
Fragments arranged by size:
2678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2676 4075 4262 1700
Size: 110 866 1399 187 2869
Fragments arranged by size:
2869 1399 866 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5431
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5431
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2130 2563 2011
Size: 119 433 4879
Fragments arranged by size:
4879 433 119
BstYI r'GATC_y
Cuts at: 1972 2316 2761 3128 3513 3530 4982 5068 5080
Size: 344 445 367 385 17 1452 86 12
Cuts at: 5080 5426 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 445 385 367 346 344 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4879
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3741 3761 2947
Size: 794 20 4617
Fragments arranged by size:
4617 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2565 2878
Size: 54 1425 50 275 18 207 528 313
Cuts at: 2878 3767 5396 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 528 313 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2585 3361 1353
Size: 666 566 776 3423
Fragments arranged by size:
3423 776 666 566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4018 4392 4538 387
Size: 1064 138 2429 374 146 1280
Fragments arranged by size:
2429 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2159 2282 2435 2462 2624 2648
Size: 393 437 90 123 153 27 162 24
Cuts at: 2648 3494 4091 4878 5146 1239
Size: 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 597 437 393 268 162
153 123 90 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2474 2606 2474
Size: 132 5299
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2408 2436 4578 1678
Size: 730 28 2142 2531
Fragments arranged by size:
2531 2142 730 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2108 2128 2140 4072
Size: 32 135 32 524 12 20 12 1932
Cuts at: 4072 4104 4146 4178 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1932 524 135 42 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3021 669
Size: 988 281 837 246 3079
Fragments arranged by size:
3079 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2661 4545 4729 1305
Size: 789 567 1884 184 2007
Fragments arranged by size:
2007 1884 789 567 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2567 10
Size: 54 1425 343 735 2874
Fragments arranged by size:
2874 1425 735 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2561 3389 3748 3907 518
Size: 648 1395 828 359 159 2042
Fragments arranged by size:
2042 1395 828 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2425 2647 2847 3524 4672 4917 1606
Size: 819 222 200 677 1148 245 2120
Fragments arranged by size:
2120 1148 819 677 245 222 200
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4879
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NsiI A_TGCA'T
Cuts at: 2169 2169
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2371 2789 4334 1132
Size: 97 1142 418 1545 2229
Fragments arranged by size:
2229 1545 1142 418 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5431
PfoI T'CCnGG_A
Cuts at: 2295 2295
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2058 2079 2783 830
Size: 1228 21 704 3478
Fragments arranged by size:
3478 1228 704 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
PvuII CAG'CTG
Cuts at: 2647 2647
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 2045 3679 5391 2045
Size: 1634 1712 2085
Fragments arranged by size:
2085 1712 1634
SfcI C'TryA_G
Cuts at: 826 1045 2054 2075 2779 3914 4595 4786 826
Size: 219 1009 21 704 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 704 681 219 191 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2758 1872
Size: 886 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 3535 4436 4698 820
Size: 197 926 411 1181 901 262 1553
Fragments arranged by size:
1553 1181 926 901 411 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2870 513
Size: 552 1805 3074
Fragments arranged by size:
3074 1805 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 3458 3475 3628 3813 4258 325
Size: 1255 1071 807 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1071 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2043 3677 5389
Size: 274 80 33 51 1443 66 1634 1712
Cuts at: 5389 96
Size: 138
Fragments arranged by size:
1712 1634 1443 274 138 80 66 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5391 5423 5391
Size: 32 5399
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2134
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2756 1870
Size: 886 4545
XmnI GAAnn'nnTTC
Cuts at: 2579 3560 2579
Size: 981 4450
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI