Restriction map for
pFN21AB6442
(Circular) (Six-base) MAPSORT of: pFN21AB6442.seq Check: 2256 from: 1 to: 5743
pFN21AB6442 5743 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2439 2439
Size: 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2870 3553 278
Size: 53 83 186 936 1334 683 2468
Fragments arranged by size:
2468 1334 936 683 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 2613 3064 2613
Size: 451 5292
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2092 2147 2318 3806 5190 5458 1632
Size: 460 55 171 1488 1384 268 1917
Fragments arranged by size:
1917 1488 1384 460 268 171 55
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 2066
Size: 32 5711
AlwNI CAG_nnn'CTG
Cuts at: 2643 3038 3486 5058 2643
Size: 395 448 1572 3328
Fragments arranged by size:
3328 1572 448 395
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2891 3068 1870
Size: 73 948 177 4545
Fragments arranged by size:
4545 948 177 73
AvrII C'CTAG_G
Cuts at: 2113 2113
Size: 5743
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2613 3064 4519 618
Size: 325 217 627 826 451 1455 1842
Fragments arranged by size:
1842 1455 826 627 451 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2378 2406 2733 3062 1391
Size: 673 314 28 327 329 4072
Fragments arranged by size:
4072 673 329 327 314 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 2471 928
Size: 971 316 256 4200
Fragments arranged by size:
4200 971 316 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3035 4354 136
Size: 107 122 71 631 605 1363 1319 1525
Fragments arranged by size:
1525 1363 1319 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2244 5738 2244
Size: 3494 2249
BlpI GC'TnA_GC
Cuts at: 2768 3160 2768
Size: 392 5351
Bme1580I G_kGCm'C
Cuts at: 1790 2187 3804 4960 1790
Size: 397 1617 1156 2573
Fragments arranged by size:
2573 1617 1156 397
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 2948 2984 4403 1239
Size: 1163 546 36 1419 2579
Fragments arranged by size:
2579 1419 1163 546 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2390 3868 4733 5031 2390
Size: 1478 865 298 3102
Fragments arranged by size:
3102 1478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2939 4406 882
Size: 617 1440 1467 2219
Fragments arranged by size:
2219 1467 1440 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 2628 2867 3550
Size: 53 83 186 936 723 372 239 683
Cuts at: 3550 3932 4567 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 723 683 635 382 372 239
186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 2552 2582 1178
Size: 30 165 30 663 30 456 30 4339
Fragments arranged by size:
4339 663 456 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2741 2804 1186
Size: 975 580 63 4125
Fragments arranged by size:
4125 975 580 63
BseYI C'CCAG_C
Cuts at: 2237 2266 2980 4946 5442 2237
Size: 29 714 1966 496 2538
Fragments arranged by size:
2538 1966 714 496 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2733 3804 3889 4960 2733
Size: 1071 85 1071 3516
Fragments arranged by size:
3516 1071 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2187 2378 2406 2733 3062 3804
Size: 399 274 123 191 28 327 329 742
Cuts at: 3804 3889 4960 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 329 327 274 191
123 85 28
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 2070 2457 2787 3632 5362 2070
Size: 387 330 845 1730 2451
Fragments arranged by size:
2451 1730 845 387 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2439 3098 844
Size: 1595 659 3489
Fragments arranged by size:
3489 1595 659
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 2833 1184
Size: 1649 4094
BstYI r'GATC_y
Cuts at: 1972 2244 3073 3440 3825 3842 5294 5380 5392
Size: 272 829 367 385 17 1452 86 12
Cuts at: 5392 5738 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 829 385 367 346 272 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2660 2796 513
Size: 552 939 656 136 3460
Fragments arranged by size:
3460 939 656 552 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2166 487
Size: 683 56 940 4064
Fragments arranged by size:
4064 940 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3190 4079 5708
Size: 54 1425 50 275 18 1360 889 1629
Cuts at: 5708 8
Size: 43
Fragments arranged by size:
1629 1425 1360 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 2345 2831 2927 3673 1353
Size: 977 15 486 96 746 3423
Fragments arranged by size:
3423 977 746 486 96 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2731 2731
Size: 5743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2147 2318 2402 2948 2984 3806
Size: 393 460 55 171 84 546 36 822
Cuts at: 3806 4403 5190 5458 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 822 787 597 546 460 393 268
171 84 55 36
EcoO109I rG'GnC_Cy
Cuts at: 3028 3028
Size: 5743
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2012 4890 1678
Size: 334 2878 2531
Fragments arranged by size:
2878 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2533 2552 2565 2584 2867
Size: 32 135 32 961 19 13 19 283
Cuts at: 2867 2899 4384 4416 4458 4490 1373
Size: 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 961 283 135 42 32 32
32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 2617 3068 2617
Size: 451 5292
MfeI C'AATT_G
Cuts at: 2844 3342 2844
Size: 498 5245
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2910 4857 5041 1305
Size: 1605 1947 184 2007
Fragments arranged by size:
2007 1947 1605 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 3701 4060 4219 518
Size: 648 1308 1227 359 159 2042
Fragments arranged by size:
2042 1308 1227 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2640 3159 3836 4984 5229 1606
Size: 1034 519 677 1148 245 2120
Fragments arranged by size:
2120 1148 1034 677 519 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2796 513
Size: 552 939 792 3460
Fragments arranged by size:
3460 939 792 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2479 3101 4646 1132
Size: 97 1250 622 1545 2229
Fragments arranged by size:
2229 1545 1250 622 97
PasI CC'CwG_GG
Cuts at: 1348 2392 1348
Size: 1044 4699
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3038 1108
Size: 1930 3813
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2098 3812 3844 5325 5357 2066
Size: 32 1714 32 1481 32 2452
Fragments arranged by size:
2452 1714 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2484 2756 3095 830
Size: 1654 272 339 3478
Fragments arranged by size:
3478 1654 339 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2640 2640
Size: 5743
SacI G_AGCT'C
Cuts at: 2733 2733
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 2831 2927 2831
Size: 96 5647
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2423 3991 5703 2423
Size: 1568 1712 2463
Fragments arranged by size:
2463 1712 1568
SexAI A'CCwGG_T
Cuts at: 2616 2994 2616
Size: 378 5365
SfcI C'TryA_G
Cuts at: 826 1045 2480 2704 2752 2776 2861 3091 4226
Size: 219 1435 224 48 24 85 230 1135
Cuts at: 4226 4907 5098 826
Size: 681 191 1471
Fragments arranged by size:
1471 1435 1135 681 230 224 219 191
85 48 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 3847 4748 5010 820
Size: 197 926 462 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 462 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2957 1517
Size: 1440 4303
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 2113 2398 2796 3182 513
Size: 552 939 52 57 285 398 386 3074
Fragments arranged by size:
3074 939 552 398 386 285 57 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2731 2789 3770 3787 3940 4125 4570
Size: 1255 1151 58 981 17 153 185 445
Cuts at: 4570 325
Size: 1498
Fragments arranged by size:
1498 1255 1151 981 445 185 153 58
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 3989 5701
Size: 274 80 33 51 1443 444 1568 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1712 1568 1443 444 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 2627 1316
Size: 1311 4432
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 3872 2078
Size: 1794 3949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2868 3551 276
Size: 53 83 186 936 1334 683 2468
Fragments arranged by size:
2468 1334 936 683 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI
XcmI