Restriction map for
pFN21AB6440
(Circular) (Six-base) MAPSORT of: pFN21AB6440.seq Check: 7631 from: 1 to: 5740
pFN21AB6440 5740 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3803 5187 5455 1632
Size: 2171 1384 268 1917
Fragments arranged by size:
2171 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 2822 1166
Size: 1656 4084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 2374 3797 4953 2374
Size: 1423 1156 3161
Fragments arranged by size:
3161 1423 1156
ApoI r'AATT_y
Cuts at: 1493 2858 3049 3275 3427 5536 1493
Size: 1365 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 1365 226 191 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2175 3065 1870
Size: 73 89 143 890 4545
Fragments arranged by size:
4545 890 143 89 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2399 3059 1391
Size: 1008 660 4072
Fragments arranged by size:
4072 1008 660
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BfrBI ATG'CAT
Cuts at: 3020 3020
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2378 3801 4957 1790
Size: 588 1423 1156 2573
Fragments arranged by size:
2573 1423 1156 588
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 2765 3471
Size: 416 53 83 399 24 932 416 706
Cuts at: 3471 4429 442
Size: 958 1753
Fragments arranged by size:
1753 958 932 706 416 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1998 4400 1239
Size: 759 2402 2579
Fragments arranged by size:
2579 2402 759
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2148 3531 493
Size: 711 944 1383 2702
Fragments arranged by size:
2702 1383 944 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2826 2856 1178
Size: 30 165 30 1423 30 4062
Fragments arranged by size:
4062 1423 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2193 1186
Size: 1007 4733
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2551 1163
Size: 573 815 4352
Fragments arranged by size:
4352 815 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2378 2419 3801 3886 4957 2022
Size: 356 41 1382 85 1071 2805
Fragments arranged by size:
2805 1382 1071 356 85 41
BsiWI C'GTAC_G
Cuts at: 2287 2287
Size: 5740
BsmI GAATG_Cn'
Cuts at: 2606 2877 3251 3344 2606
Size: 271 374 93 5002
Fragments arranged by size:
5002 374 271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2378 2399 2419 3059 3801 3886
Size: 399 232 356 21 20 640 742 85
Cuts at: 3886 4957 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 640 399 356 232 85
21 20
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2573 3095 844
Size: 1729 522 3489
Fragments arranged by size:
3489 1729 522
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2240 96
Size: 2144 3596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 3070 3437 3822 3839 5291 5377 5389 5735
Size: 1098 367 385 17 1452 86 12 346
Cuts at: 5735 1972
Size: 1977
Fragments arranged by size:
1977 1452 1098 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2555 487
Size: 683 56 1329 3672
Fragments arranged by size:
3672 1329 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DraIII CAC_nnn'GTG
Cuts at: 2465 2465
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2070 4747 809
Size: 507 754 2677 1802
Fragments arranged by size:
2677 1802 754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3187 4076 5705
Size: 54 1425 50 275 18 1357 889 1629
Cuts at: 5705 8
Size: 43
Fragments arranged by size:
1629 1425 1357 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 3670 1353
Size: 780 1537 3423
Fragments arranged by size:
3423 1537 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 3803 4400 5187 5455 1239
Size: 393 366 1805 597 787 268 1524
Fragments arranged by size:
1805 1524 787 597 393 366 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2208 2208
Size: 5740
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 2361 4887 1678
Size: 683 2526 2531
Fragments arranged by size:
2531 2526 683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373
Size: 32 135 32 2809 32 42 32 2626
Fragments arranged by size:
2809 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4854 5038 1305
Size: 3549 184 2007
Fragments arranged by size:
3549 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 3698 4057 4216 518
Size: 648 1656 876 359 159 2042
Fragments arranged by size:
2042 1656 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2483 3156 3833 4981 5226 1606
Size: 877 673 677 1148 245 2120
Fragments arranged by size:
2120 1148 877 677 673 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NsiI A_TGCA'T
Cuts at: 3022 3022
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 2540 3098 4643 1132
Size: 97 1311 558 1545 2229
Fragments arranged by size:
2229 1545 1311 558 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2483 2642 1108
Size: 1375 159 4206
Fragments arranged by size:
4206 1375 159
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 2472 3310 2472
Size: 838 4902
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2483 2483
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2032 3844 4745 5007 820
Size: 197 926 89 1812 901 262 1553
Fragments arranged by size:
1812 1553 926 901 262 197 89
SnaBI TAC'GTA
Cuts at: 493 2148 3531 493
Size: 1655 1383 2702
Fragments arranged by size:
2702 1655 1383
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2830 3179 513
Size: 552 1765 349 3074
Fragments arranged by size:
3074 1765 552 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2260 3767 3784 3937 4122 4567 325
Size: 1255 680 1507 17 153 185 445 1498
Fragments arranged by size:
1507 1498 1255 680 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2240 3986 5698
Size: 274 80 33 51 1443 263 1746 1712
Cuts at: 5698 96
Size: 138
Fragments arranged by size:
1746 1712 1443 274 263 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2906 2930 2938 2962 5700 5732 2906
Size: 24 8 24 2738 32 2914
Fragments arranged by size:
2914 2738 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 2032 1943
Size: 89 5651
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 2491 3869 2491
Size: 1378 4362
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI