Restriction map for
pFN21AB6438
(Circular) (Six-base) MAPSORT of: pFN21AB6438.seq Check: 7127 from: 1 to: 5725
pFN21AB6438 5725 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2276 2652 2276
Size: 376 5349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 2873 2875 3788 5172 5440 1632
Size: 896 345 2 913 1384 268 1917
Fragments arranged by size:
1917 1384 913 896 345 268 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 2190 4624 5601 1203
Size: 987 2434 977 1327
Fragments arranged by size:
2434 1327 987 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4534
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2258 1184
Size: 1074 4651
AlwNI CAG_nnn'CTG
Cuts at: 2800 2962 3468 5040 2800
Size: 162 506 1572 3485
Fragments arranged by size:
3485 1572 506 162
ApaI G_GGCC'C
Cuts at: 2181 2316 2579 2826 2181
Size: 135 263 247 5080
Fragments arranged by size:
5080 263 247 135
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2316 3050 1870
Size: 73 373 734 4545
Fragments arranged by size:
4545 734 373 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2114 2601 3046 4501
Size: 325 217 627 257 70 487 445 1455
Cuts at: 4501 618
Size: 1842
Fragments arranged by size:
1842 1455 627 487 445 325 257 217
70
BanII G_rGCy'C
Cuts at: 1391 2025 2181 2316 2579 2826 3044 1391
Size: 634 156 135 263 247 218 4072
Fragments arranged by size:
4072 634 263 247 218 156 135
BbeI G_GCGC'C
Cuts at: 2605 2605
Size: 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2933 3617 4827 2933
Size: 684 1210 3831
Fragments arranged by size:
3831 1210 684
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2176 2803 4336
Size: 107 122 71 631 605 504 627 1533
Cuts at: 4336 136
Size: 1525
Fragments arranged by size:
1533 1525 631 627 605 504 122 107
71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 2748 3142 2748
Size: 394 5331
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2181 2316 2535 2579 2591 2671 2826
Size: 327 64 135 219 44 12 80 155
Cuts at: 2826 3786 4942 1790
Size: 960 1156 2573
Fragments arranged by size:
2573 1156 960 327 219 155 135 80
64 44 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2820 2890 3456
Size: 416 53 83 399 24 1403 70 566
Cuts at: 3456 4414 442
Size: 958 1753
Fragments arranged by size:
1753 1403 958 566 416 399 83 70
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2521 4385 1239
Size: 863 419 1864 2579
Fragments arranged by size:
2579 1864 863 419
Bpu10I CC'TnA_GC
Cuts at: 2019 2840 2019
Size: 821 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2382 3850 4715 5013 2382
Size: 1468 865 298 3094
Fragments arranged by size:
3094 1468 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2193 2672 2921 3516 493
Size: 711 989 479 249 595 2702
Fragments arranged by size:
2702 989 711 595 479 249
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 2602 2886 3532
Size: 53 83 186 936 693 376 284 646
Cuts at: 3532 3914 4549 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 693 646 635 382 376 284
186 83 53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2951 2954 2957 1186
Size: 1765 3 3 3954
Fragments arranged by size:
3954 1765 3 3
BseYI C'CCAG_C
Cuts at: 2051 2060 2328 4928 5424 2051
Size: 9 268 2600 496 2352
Fragments arranged by size:
2600 2352 496 268 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2990 1163
Size: 573 1254 3898
Fragments arranged by size:
3898 1254 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2949 3786 3871 4942 2025
Size: 924 837 85 1071 2808
Fragments arranged by size:
2808 1071 924 837 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2117 2181 2316 2535 2579 2591
Size: 399 235 92 64 135 219 44 12
Cuts at: 2591 2671 2826 2949 3044 3786 3871 4942 1391
Size: 80 155 123 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 235 219 155 135
123 95 92 85 80 64 44 12
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 2457 3614 5344 2457
Size: 1157 1730 2838
Fragments arranged by size:
2838 1730 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2276 2628 2652 3080 844
Size: 1432 352 24 428 3489
Fragments arranged by size:
3489 1432 428 352 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2347 3612 1394
Size: 429 524 1265 3507
Fragments arranged by size:
3507 1265 524 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2254 4220 4394 65
Size: 1183 1006 1966 174 1396
Fragments arranged by size:
1966 1396 1183 1006 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 2056 2250 2286 2743 1925
Size: 131 194 36 457 4907
Fragments arranged by size:
4907 457 194 131 36
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2121 2800 794
Size: 1327 679 3719
Fragments arranged by size:
3719 1327 679
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2958 2991 3055 3422 3807 3824 5276 5362
Size: 986 33 64 367 385 17 1452 86
Cuts at: 5362 5374 5720 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 986 385 367 346 86 64
33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2152 2172 2337 513
Size: 552 1087 20 165 3901
Fragments arranged by size:
3901 1087 552 165 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 487
Size: 683 56 863 4123
Fragments arranged by size:
4123 863 683 56
BtsI GCAGTG_nn'
Cuts at: 2252 3241 4035 4055 2252
Size: 989 794 20 3922
Fragments arranged by size:
3922 989 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2538 2573 529
Size: 35 1974 35 3681
Fragments arranged by size:
3681 1974 35 35
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DraIII CAC_nnn'GTG
Cuts at: 2121 2196 2121
Size: 75 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2149 2155 2758
Size: 54 1425 50 275 18 319 6 603
Cuts at: 2758 3172 4061 5690 8
Size: 414 889 1629 43
Fragments arranged by size:
1629 1425 889 603 414 319 275 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 3655 1353
Size: 1106 1196 3423
Fragments arranged by size:
3423 1196 1106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2942 4312 4686 4832 387
Size: 1064 138 1353 1370 374 146 1280
Fragments arranged by size:
1370 1353 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2023
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2521 2528 2873 2875 3788 4385
Size: 393 470 419 7 345 2 913 597
Cuts at: 4385 5172 5440 1239
Size: 787 268 1524
Fragments arranged by size:
1524 913 787 597 470 419 393 345
268 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2624 2624
Size: 5725
EcoO109I rG'GnC_Cy
Cuts at: 2177 2313 2320 2575 2686 2822 2836 3001 2177
Size: 136 7 255 111 136 14 165 4901
Fragments arranged by size:
4901 255 165 136 136 111 14 7
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 2102 2234 4231 2102
Size: 132 1997 3596
Fragments arranged by size:
3596 1997 132
HaeII r_GCGC'y
Cuts at: 1678 2605 4872 1678
Size: 927 2267 2531
Fragments arranged by size:
2531 2267 927
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KasI G'GCGC_C
Cuts at: 2601 2601
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2763 4839 5023 1305
Size: 1458 2076 184 2007
Fragments arranged by size:
2076 2007 1458 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 10
Size: 54 1425 343 325 3578
Fragments arranged by size:
3578 1425 343 325 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2646 3683 4042 4201 518
Size: 648 1191 289 1037 359 159 2042
Fragments arranged by size:
2042 1191 1037 648 359 289 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2140 2174 2280 2339 2506 2932 3141
Size: 445 89 34 106 59 167 426 209
Cuts at: 3141 3818 4966 5211 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 445 426 245 209 167
106 89 59 34
NarI GG'CG_CC
Cuts at: 2602 2602
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2651 2899 3083 4628 1132
Size: 97 1422 248 184 1545 2229
Fragments arranged by size:
2229 1545 1422 248 184 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PmlI CAC'GTG
Cuts at: 2193 2672 2193
Size: 479 5246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 2320
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PspOMI G'GGCC_C
Cuts at: 2177 2312 2575 2822 2177
Size: 135 263 247 5080
Fragments arranged by size:
5080 263 247 135
PstI C_TGCA'G
Cuts at: 830 2247 2931 2967 3077 830
Size: 1417 684 36 110 3478
Fragments arranged by size:
3478 1417 684 110 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2140 2506 2140
Size: 366 5359
SacI G_AGCT'C
Cuts at: 2025 2025
Size: 5725
SacII CC_GC'GG
Cuts at: 2175 2340 2175
Size: 165 5560
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 2972 3973 5685 2972
Size: 1001 1712 3012
Fragments arranged by size:
3012 1712 1001
SfcI C'TryA_G
Cuts at: 826 1045 2243 2927 2963 3073 4208 4889 5080
Size: 219 1198 684 36 110 1135 681 191
Cuts at: 5080 826
Size: 1471
Fragments arranged by size:
1471 1198 1135 684 681 219 191 110
36
SfoI GGC'GCC
Cuts at: 2603 2603
Size: 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 2318 3052 1872
Size: 446 734 4545
Fragments arranged by size:
4545 734 446
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2397 3829 4730 4992 820
Size: 197 926 454 1432 901 262 1553
Fragments arranged by size:
1553 1432 926 901 454 262 197
SnaBI TAC'GTA
Cuts at: 493 2921 3516 493
Size: 2428 595 2702
Fragments arranged by size:
2702 2428 595
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 2899 3083 1132
Size: 1767 184 3774
Fragments arranged by size:
3774 1767 184
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 2241 1517
Size: 724 5001
StyI C'CwwG_G
Cuts at: 513 1065 2625 3019 3164 513
Size: 552 1560 394 145 3074
Fragments arranged by size:
3074 1560 552 394 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 2766 2970
Size: 274 80 33 51 1443 450 339 204
Cuts at: 2970 3971 5683 96
Size: 1001 1712 138
Fragments arranged by size:
1712 1443 1001 450 339 274 204 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5685 5717 5685
Size: 32 5693
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2137 2137
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 2316 3050 1870
Size: 446 734 4545
Fragments arranged by size:
4545 734 446
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI