Restriction map for
pFN21AB6436
(Circular) (Six-base) MAPSORT of: pFN21AB6436.seq Check: 3311 from: 1 to: 5638
pFN21AB6436 5638 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2828 3701 5085 5353 1632
Size: 1196 873 1384 268 1917
Fragments arranged by size:
1917 1384 1196 873 268
AflII C'TTAA_G
Cuts at: 820 1017 2288 820
Size: 197 1271 4170
Fragments arranged by size:
4170 1271 197
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 2935 4469 2935
Size: 1534 4104
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2383 2436 1184
Size: 1199 53 4386
Fragments arranged by size:
4386 1199 53
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaLI G'TGCA_C
Cuts at: 2421 3695 4851 2421
Size: 1274 1156 3208
Fragments arranged by size:
3208 1274 1156
ApoI r'AATT_y
Cuts at: 1493 2477 2622 2774 2903 2947 3173 3325 5434
Size: 984 145 152 129 44 226 152 2109
Cuts at: 5434 1493
Size: 1697
Fragments arranged by size:
2109 1697 984 226 152 152 145 129
44
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2922 2955 2922
Size: 33 5605
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2756 2959 4414 618
Size: 325 217 627 266 703 203 1455 1842
Fragments arranged by size:
1842 1455 703 627 325 266 217 203
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbeI G_GCGC'C
Cuts at: 2057 2057
Size: 5638
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2848 928
Size: 971 949 3718
Fragments arranged by size:
3718 971 949
BbvCI CC'TCA_GC
Cuts at: 2790 2790
Size: 5638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 2051 2062
Size: 107 122 71 631 605 346 33 11
Cuts at: 2062 4249 136
Size: 2187 1525
Fragments arranged by size:
2187 1525 631 605 346 122 107 71
33 11
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2425 3699 4855 1790
Size: 635 1274 1156 2573
Fragments arranged by size:
2573 1274 1156 635
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2831 2848 3369
Size: 416 53 83 399 24 1414 17 521
Cuts at: 3369 4327 442
Size: 958 1753
Fragments arranged by size:
1753 1414 958 521 416 399 83 53
24 17
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2329 2416 4298 1239
Size: 1028 62 87 1882 2579
Fragments arranged by size:
2579 1882 1028 87 62
Bpu10I CC'TnA_GC
Cuts at: 2790 2790
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2709 3763 4628 4926 2594
Size: 115 1054 865 298 3306
Fragments arranged by size:
3306 1054 865 298 115
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 2124 3445 3827
Size: 53 83 186 936 521 70 1321 382
Cuts at: 3827 4462 275
Size: 635 1451
Fragments arranged by size:
1451 1321 936 635 521 382 186 83
70 53
BsaWI w'CCGG_w
Cuts at: 2935 4071 4469 4743 4890 5301 2935
Size: 1136 398 274 147 411 3272
Fragments arranged by size:
3272 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2259 2289 2548 2578 1178
Size: 30 165 30 856 30 259 30 4238
Fragments arranged by size:
4238 856 259 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 2035 2087 4841 5337 2035
Size: 52 2754 496 2336
Fragments arranged by size:
2754 2336 496 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2302 2345 2425 3699 3784 4855 2302
Size: 43 80 1274 85 1071 3085
Fragments arranged by size:
3085 1274 1071 85 80 43
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2302 2345 2425 2957 3699 3784 4855
Size: 399 512 43 80 532 742 85 1071
Cuts at: 4855 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 532 512 399 85 80
43
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2177 2935 4282 4469 1700
Size: 110 246 121 758 1347 187 2869
Fragments arranged by size:
2869 1347 758 246 187 121 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633
Size: 996 367 385 17 1452 86 12 346
Cuts at: 5633 1972
Size: 1977
Fragments arranged by size:
1977 1452 996 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2517 2517
Size: 5638
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 3559
Fragments arranged by size:
3559 939 588 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2638 2942 2638
Size: 304 5334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 3085 3974
Size: 54 1425 50 275 18 283 972 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 972 889 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 3568 1353
Size: 800 1415 3423
Fragments arranged by size:
3423 1415 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2329 2416 2828 3701 4298 5085
Size: 393 635 62 87 412 873 597 787
Cuts at: 5085 5353 1239
Size: 268 1524
Fragments arranged by size:
1524 873 787 635 597 412 393 268
87 62
EcoO109I rG'GnC_Cy
Cuts at: 2330 2330
Size: 5638
EcoRI G'AATT_C
Cuts at: 2774 2947 3325 2774
Size: 173 378 5087
Fragments arranged by size:
5087 378 173
FspI TGC'GCA
Cuts at: 2072 4144 2072
Size: 2072 3566
HaeII r_GCGC'y
Cuts at: 1678 2057 4785 1678
Size: 379 2728 2531
Fragments arranged by size:
2728 2531 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 4279 4311 4353
Size: 32 135 32 638 32 2037 32 42
Cuts at: 4353 4385 1373
Size: 32 2626
Fragments arranged by size:
2626 2037 638 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KasI G'GCGC_C
Cuts at: 2053 2053
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3816
Fragments arranged by size:
3816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3596 3955 4114 518
Size: 648 2430 359 159 2042
Fragments arranged by size:
2430 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2136 3054 3731 4879 5124 1606
Size: 501 29 918 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 677 501 245 29
NaeI GCC'GGC
Cuts at: 2058 2179 2058
Size: 121 5517
NarI GG'CG_CC
Cuts at: 2054 2054
Size: 5638
NcoI C'CATG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 3559
Fragments arranged by size:
3559 939 588 552
NdeI CA'TA_TG
Cuts at: 387 2230 387
Size: 1843 3795
NgoMIV G'CCGG_C
Cuts at: 2056 2177 2056
Size: 121 5517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2312 2372 2879 2990 830
Size: 1482 60 507 111 3478
Fragments arranged by size:
3478 1482 507 111 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2308 2368 2875 2986 4121 4802 4993
Size: 219 1263 60 507 111 1135 681 191
Cuts at: 4993 826
Size: 1471
Fragments arranged by size:
1471 1263 1135 681 507 219 191 111
60
SfoI GGC'GCC
Cuts at: 2055 2055
Size: 5638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 2056 1700
Size: 356 5282
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 2573 2724 3742 4643 4905
Size: 197 926 345 285 151 1018 901 262
Cuts at: 4905 820
Size: 1553
Fragments arranged by size:
1553 1018 926 901 345 285 262 197
151
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 2719 2769 3562 5
Size: 47 2667 50 793 2081
Fragments arranged by size:
2667 2081 793 50 47
StuI AGG'CCT
Cuts at: 1517 2026 1517
Size: 509 5129
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 3077 513
Size: 552 939 588 485 3074
Fragments arranged by size:
3074 939 588 552 485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2552 3665 3682 3835 4020 4465 325
Size: 1255 972 1113 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1113 972 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3884 5596 96
Size: 274 80 33 51 1443 1907 1712 138
Fragments arranged by size:
1907 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2259 2291 2548 2580 2695 2727 5598 5630 2259
Size: 32 257 32 115 32 2871 32 2267
Fragments arranged by size:
2871 2267 257 115 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2767 2291
Size: 476 5162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 2667 2718 3767 2667
Size: 51 1049 4538
Fragments arranged by size:
4538 1049 51
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI