Restriction map for
pFN21AB6434
(Circular) (Six-base) MAPSORT of: pFN21AB6434.seq Check: 7455 from: 1 to: 5737
pFN21AB6434 5737 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 2200 3058 2200
Size: 858 4879
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2692 2861 3800 5184 5452 1632
Size: 1060 169 939 1384 268 1917
Fragments arranged by size:
1917 1384 1060 939 268 169
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 5705
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaLI G'TGCA_C
Cuts at: 3794 4950 3794
Size: 1156 4581
ApoI r'AATT_y
Cuts at: 1493 3046 3272 3424 5533 1493
Size: 1553 226 152 2109 1697
Fragments arranged by size:
2109 1697 1553 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 3062 1870
Size: 73 813 306 4545
Fragments arranged by size:
4545 813 306 73
AvrII C'CTAG_G
Cuts at: 2956 2956
Size: 5737
BamHI G'GATC_C
Cuts at: 2191 2259 3067 3434 2191
Size: 68 808 367 4494
Fragments arranged by size:
4494 808 367 68
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 2584 2976 3058 4513
Size: 325 217 627 413 384 392 82 1455
Cuts at: 4513 618
Size: 1842
Fragments arranged by size:
1842 1455 627 413 392 384 325 217
82
BanII G_rGCy'C
Cuts at: 1391 2816 3056 1391
Size: 1425 240 4072
Fragments arranged by size:
4072 1425 240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4766
BbvCI CC'TCA_GC
Cuts at: 2276 2924 2276
Size: 648 5089
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2722 2756 3928 3962 1903
Size: 34 785 34 1172 34 3678
Fragments arranged by size:
3678 1172 785 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2595 3629 4839 2595
Size: 1034 1210 3493
Fragments arranged by size:
3493 1210 1034
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2593 4348 136
Size: 107 122 71 631 605 921 1755 1525
Fragments arranged by size:
1755 1525 921 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 2676 3154 2676
Size: 478 5259
Bme1580I G_kGCm'C
Cuts at: 1790 2979 3798 4954 1790
Size: 1189 819 1156 2573
Fragments arranged by size:
2573 1189 1156 819
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2387 2387
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2887 4397 1239
Size: 1648 1510 2579
Fragments arranged by size:
2579 1648 1510
Bpu10I CC'TnA_GC
Cuts at: 2276 2924 2276
Size: 648 5089
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4727 5025 3862
Size: 865 298 4574
Fragments arranged by size:
4574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2679 4400 882
Size: 617 1180 1721 2219
Fragments arranged by size:
2219 1721 1180 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 2264 5731 2264
Size: 3467 2270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3544 3926 4561 275
Size: 53 83 186 936 2011 382 635 1451
Fragments arranged by size:
2011 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2353 4170 4568 4842 4989 5400 2353
Size: 1817 398 274 147 411 2690
Fragments arranged by size:
2690 1817 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2885 2915 1178
Size: 30 165 30 1482 30 4000
Fragments arranged by size:
4000 1482 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2526 2923 2972 1186
Size: 1099 241 397 49 3951
Fragments arranged by size:
3951 1099 397 241 49
BseYI C'CCAG_C
Cuts at: 2439 2645 2717 2728 2829 4940 5436 2439
Size: 206 72 11 101 2111 496 2740
Fragments arranged by size:
2740 2111 496 206 101 72 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2373 2677 3798 3883 4954 2108
Size: 265 304 1121 85 1071 2891
Fragments arranged by size:
2891 1121 1071 304 265 85
BsmI GAATG_Cn'
Cuts at: 3248 3341 3248
Size: 93 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2373 2677 2816 2979 3056 3798
Size: 399 318 265 304 139 163 77 742
Cuts at: 3798 3883 4954 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 318 304 265 163
139 85 77
BspEI T'CCGG_A
Cuts at: 5400 5400
Size: 5737
BspHI T'CATG_A
Cuts at: 2539 3626 5356 2539
Size: 1087 1730 2920
Fragments arranged by size:
2920 1730 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2628 3092 844
Size: 1784 464 3489
Fragments arranged by size:
3489 1784 464
BsrBI CCG'CTC
Cuts at: 1394 1823 2232 2939 3624 1394
Size: 429 409 707 685 3507
Fragments arranged by size:
3507 707 685 429 409
BsrDI GCAATG_nn'
Cuts at: 65 1248 3009 4232 4406 65
Size: 1183 1761 1223 174 1396
Fragments arranged by size:
1761 1396 1223 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2330 4381 4568 1700
Size: 110 520 2051 187 2869
Fragments arranged by size:
2869 2051 520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2672 794
Size: 1878 3859
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 2079 2191 2259 2880 3067 3434 3819 3836
Size: 107 112 68 621 187 367 385 17
Cuts at: 3836 5288 5374 5386 5732 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 621 385 367 346 187 112
107 86 68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2988 513
Size: 552 939 984 3262
Fragments arranged by size:
3262 984 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2323 487
Size: 683 56 1097 3901
Fragments arranged by size:
3901 1097 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 2754 3253 4047 4067 2499
Size: 255 499 794 20 4169
Fragments arranged by size:
4169 794 499 255 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3184 4073
Size: 54 1425 50 275 18 177 1177 889
Cuts at: 4073 5702 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1177 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3667 1353
Size: 1466 848 3423
Fragments arranged by size:
3423 1466 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2692 2861 2887 3800 4397 5184 5452
Size: 393 1060 169 26 913 597 787 268
Cuts at: 5452 1239
Size: 1524
Fragments arranged by size:
1524 1060 913 787 597 393 268 169
26
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5737
EcoO109I rG'GnC_Cy
Cuts at: 2053 2053
Size: 5737
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
EcoRV GAT'ATC
Cuts at: 2545 2545
Size: 5737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2870 2870
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 2864 3003 4884 1678
Size: 1186 139 1881 2531
Fragments arranged by size:
2531 1881 1186 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 2593 2625 2784
Size: 32 135 32 465 32 524 32 159
Cuts at: 2784 2816 2872 2904 4378 4410 4452 4484 1373
Size: 32 56 32 1474 32 42 32 2626
Fragments arranged by size:
2626 1474 524 465 159 135 56 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2212 748
Size: 1464 4273
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 2204 3062 2204
Size: 858 4879
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2415 4851 5035 1305
Size: 1110 2436 184 2007
Fragments arranged by size:
2436 2007 1110 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2915 3695 4054 4213 518
Size: 648 1749 780 359 159 2042
Fragments arranged by size:
2042 1749 780 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 3153 3830 4978 5223 1606
Size: 600 947 677 1148 245 2120
Fragments arranged by size:
2120 1148 947 677 600 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 5737
NcoI C'CATG_G
Cuts at: 513 1065 2004 2988 513
Size: 552 939 984 3262
Fragments arranged by size:
3262 984 939 552
NdeI CA'TA_TG
Cuts at: 387 2448 387
Size: 2061 3676
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 3095 4640 1132
Size: 97 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2869 1348
Size: 1521 4216
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 1108
Size: 1344 4393
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 3806 3838 5319 5351 2885
Size: 32 889 32 1481 32 3271
Fragments arranged by size:
3271 1481 889 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2053
Size: 5737
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PstI C_TGCA'G
Cuts at: 830 2399 3089 830
Size: 1569 690 3478
Fragments arranged by size:
3478 1569 690
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SapI GCTCTTCn'nnn_
Cuts at: 2819 2819
Size: 5737
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2395 2995 3085 4220 4901 5092 826
Size: 219 1350 600 90 1135 681 191 1471
Fragments arranged by size:
1471 1350 1135 681 600 219 191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3841 4742 5004 820
Size: 197 926 1898 901 262 1553
Fragments arranged by size:
1898 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2004 2956 2988 3176 513
Size: 552 939 952 32 188 3074
Fragments arranged by size:
3074 952 939 552 188 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2298 2444 2933 3764 3781 3934 4119
Size: 1255 718 146 489 831 17 153 185
Cuts at: 4119 4564 325
Size: 445 1498
Fragments arranged by size:
1498 1255 831 718 489 445 185 153
146 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3983 5695 96
Size: 274 80 33 51 1443 2006 1712 138
Fragments arranged by size:
2006 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5697 5729 5697
Size: 32 5705
Tth111I GACn'n_nGTC
Cuts at: 1316 2768 1316
Size: 1452 4285
XbaI T'CTAG_A
Cuts at: 3073 3073
Size: 5737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2786 2806 2786
Size: 20 5717
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 2850 2897 3866 2850
Size: 47 969 4721
Fragments arranged by size:
4721 969 47
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI