Restriction map for
pFN21AB6432
(Circular) (Six-base) MAPSORT of: pFN21AB6432.seq Check: 7348 from: 1 to: 5737
pFN21AB6432 5737 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 2703 3800 5184 5452 1632
Size: 623 448 1097 1384 268 1917
Fragments arranged by size:
1917 1384 1097 623 448 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 2796 4466 2796
Size: 1670 4067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaLI G'TGCA_C
Cuts at: 2223 3794 4950 2223
Size: 1571 1156 3010
Fragments arranged by size:
3010 1571 1156
ApoI r'AATT_y
Cuts at: 1493 2357 2546 2688 3046 3272 3424 5533 1493
Size: 864 189 142 358 226 152 2109 1697
Fragments arranged by size:
2109 1697 864 358 226 189 152 142
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2082 3062 1870
Size: 73 89 50 980 4545
Fragments arranged by size:
4545 980 89 73 50
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3058 4513 618
Size: 325 217 627 1271 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3056 1391
Size: 1665 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 1903
Size: 34 1991 34 3678
Fragments arranged by size:
3678 1991 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 3154 3154
Size: 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2974 3798 4954 1790
Size: 437 747 824 1156 2573
Fragments arranged by size:
2573 1156 824 747 437
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2168 4397 1239
Size: 836 93 2229 2579
Fragments arranged by size:
2579 2229 836 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3862 4727 5025 2858
Size: 1004 865 298 3570
Fragments arranged by size:
3570 1004 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4400 882
Size: 617 2901 2219
Fragments arranged by size:
2901 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3528 493
Size: 711 1431 893 2702
Fragments arranged by size:
2702 1431 893 711
BsaBI GATnn'nnATC
Cuts at: 2568 5731 2568
Size: 3163 2574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3544 3926 4561 275
Size: 53 83 186 936 2011 382 635 1451
Fragments arranged by size:
2011 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4170 4568 4842 4989 5400 4170
Size: 398 274 147 411 4507
Fragments arranged by size:
4507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2970 3000 1178
Size: 30 165 30 1567 30 3915
Fragments arranged by size:
3915 1567 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2978 1186
Size: 1117 675 3945
Fragments arranged by size:
3945 1117 675
BseYI C'CCAG_C
Cuts at: 4940 5436 4940
Size: 496 5241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 2227 3798 3883 4954 2094
Size: 133 1571 85 1071 2877
Fragments arranged by size:
2877 1571 1071 133 85
BsmI GAATG_Cn'
Cuts at: 2198 3248 3341 2198
Size: 1050 93 4594
Fragments arranged by size:
4594 1050 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2227 2974 3056 3798 3883 4954
Size: 399 304 133 747 82 742 85 1071
Cuts at: 4954 1391
Size: 2174
Fragments arranged by size:
2174 1071 747 742 399 304 133 85
82
BspEI T'CCGG_A
Cuts at: 5400 5400
Size: 5737
BspHI T'CATG_A
Cuts at: 3626 5356 3626
Size: 1730 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3092 844
Size: 2248 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2210 2560 4232 4406 65
Size: 1183 962 350 1672 174 1396
Fragments arranged by size:
1672 1396 1183 962 350 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4381 4568 1700
Size: 110 2571 187 2869
Fragments arranged by size:
2869 2571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5737
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 2430 3067 3434 3819 3836 5288 5374 5386
Size: 458 637 367 385 17 1452 86 12
Cuts at: 5386 5732 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 637 458 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 3015 513
Size: 552 939 12 999 3235
Fragments arranged by size:
3235 999 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 4047 4067 3253
Size: 794 20 4923
Fragments arranged by size:
4923 794 20
ClaI AT'CG_AT
Cuts at: 1554 2169 1554
Size: 615 5122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 2626 3041 2626
Size: 415 5322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3184 4073 5702
Size: 54 1425 50 275 18 1354 889 1629
Cuts at: 5702 8
Size: 43
Fragments arranged by size:
1629 1425 1354 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2304 3667 1353
Size: 951 1363 3423
Fragments arranged by size:
3423 1363 951
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2168 2255 2703 3800 4397 5184
Size: 393 443 93 87 448 1097 597 787
Cuts at: 5184 5452 1239
Size: 268 1524
Fragments arranged by size:
1524 1097 787 597 448 443 393 268
93 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 2378
Size: 5737
EcoRI G'AATT_C
Cuts at: 2688 3046 3424 2688
Size: 358 378 5001
Fragments arranged by size:
5001 378 358
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 4884 1678
Size: 3206 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2782 2814 4378 4410 4452
Size: 32 135 32 1210 32 1564 32 42
Cuts at: 4452 4484 1373
Size: 32 2626
Fragments arranged by size:
2626 1564 1210 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2893 748
Size: 2145 3592
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2107 4851 5035 1305
Size: 802 2744 184 2007
Fragments arranged by size:
2744 2007 802 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3695 4054 4213 518
Size: 648 2529 359 159 2042
Fragments arranged by size:
2529 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 3153 3830 4978 5223 1606
Size: 434 1113 677 1148 245 2120
Fragments arranged by size:
2120 1148 1113 677 434 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3015 513
Size: 552 939 1011 3235
Fragments arranged by size:
3235 1011 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 2722 3095 4640 1132
Size: 97 1493 373 1545 2229
Fragments arranged by size:
2229 1545 1493 373 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5737
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5737
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PstI C_TGCA'G
Cuts at: 830 3089 830
Size: 2259 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2745 3085 4220 4901 5092 826
Size: 219 1700 340 1135 681 191 1471
Fragments arranged by size:
1700 1471 1135 681 340 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2837 3841 4742 5004 820
Size: 197 926 894 1004 901 262 1553
Fragments arranged by size:
1553 1004 926 901 894 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 2722 3095 1132
Size: 1590 373 3774
Fragments arranged by size:
3774 1590 373
SspI AAT'ATT
Cuts at: 5 52 2767 2879 3661 5
Size: 47 2715 112 782 2081
Fragments arranged by size:
2715 2081 782 112 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2004 3015 3176 513
Size: 552 939 1011 161 3074
Fragments arranged by size:
3074 1011 939 552 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3764 3781 3934 4119 4564 325
Size: 1255 2184 17 153 185 445 1498
Fragments arranged by size:
2184 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3983 5695 96
Size: 274 80 33 51 1443 2006 1712 138
Fragments arranged by size:
2006 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5697 5729 5697
Size: 32 5705
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 2645 3073 2645
Size: 428 5309
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 2103 2451 2688 2880 3866 2103
Size: 348 237 192 986 3974
Fragments arranged by size:
3974 986 348 237 192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI