Restriction map for
pFN21AB6431
(Circular) (Six-base) MAPSORT of: pFN21AB6431.seq Check: 661 from: 1 to: 5641
pFN21AB6431 5641 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2391 2962 2391
Size: 571 5070
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2604 2726 1166
Size: 1438 122 4081
Fragments arranged by size:
4081 1438 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2194 2401 2464 2624 3384 4956 2194
Size: 207 63 160 760 1572 2879
Fragments arranged by size:
2879 1572 760 207 160 63
ApaI G_GGCC'C
Cuts at: 2661 2661
Size: 5641
ApaLI G'TGCA_C
Cuts at: 2729 2882 3698 4854 2729
Size: 153 816 1156 3516
Fragments arranged by size:
3516 1156 816 153
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2111 2966 1870
Size: 73 135 33 855 4545
Fragments arranged by size:
4545 855 135 73 33
AvrII C'CTAG_G
Cuts at: 2816 2816
Size: 5641
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2375 2408 2375
Size: 33 5608
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2254 2391 2450 2962 4417
Size: 325 217 627 467 137 59 512 1455
Cuts at: 4417 618
Size: 1842
Fragments arranged by size:
1842 1455 627 512 467 325 217 137
59
BanII G_rGCy'C
Cuts at: 1391 2112 2216 2247 2271 2661 2960 1391
Size: 721 104 31 24 390 299 4072
Fragments arranged by size:
4072 721 390 299 104 31 24
BbeI G_GCGC'C
Cuts at: 2454 2454
Size: 5641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BbvCI CC'TCA_GC
Cuts at: 2750 2750
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2627 4252 136
Size: 107 122 71 631 605 955 1625 1525
Fragments arranged by size:
1625 1525 955 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2241 3058 2241
Size: 817 4824
Bme1580I G_kGCm'C
Cuts at: 1790 2374 2661 2699 2733 2886 3702 4858 1790
Size: 584 287 38 34 153 816 1156 2573
Fragments arranged by size:
2573 1156 816 584 287 153 38 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2350 2404 2569 2822 4301 1239
Size: 1111 54 165 253 1479 2579
Fragments arranged by size:
2579 1479 1111 253 165 54
Bpu10I CC'TnA_GC
Cuts at: 2750 2750
Size: 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 2234 3766 4631 4929 2059
Size: 175 1532 865 298 2771
Fragments arranged by size:
2771 1532 865 298 175
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 2942 4304 882
Size: 617 1281 162 1362 2219
Fragments arranged by size:
2219 1362 1281 617 162
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2451 3448 3830 4465
Size: 53 83 186 936 918 997 382 635
Cuts at: 4465 275
Size: 1451
Fragments arranged by size:
1451 997 936 918 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2121 4074 4472 4746 4893 5304 2121
Size: 1953 398 274 147 411 2458
Fragments arranged by size:
2458 1953 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2566 2798 2819 1186
Size: 934 446 232 21 4008
Fragments arranged by size:
4008 934 446 232 21
BseYI C'CCAG_C
Cuts at: 2084 2518 2617 4844 5340 2084
Size: 434 99 2227 496 2385
Fragments arranged by size:
2385 2227 496 434 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2928 3091 3852 4880 1540
Size: 275 186 927 163 761 1028 2301
Fragments arranged by size:
2301 1028 927 761 275 186 163
BsiHKAI G_wGCw'C
Cuts at: 2355 2733 2886 3702 3787 4858 2355
Size: 378 153 816 85 1071 3138
Fragments arranged by size:
3138 1071 816 378 153 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2216 2247 2271 2355 2374 2661
Size: 399 322 104 31 24 84 19 287
Cuts at: 2661 2699 2733 2886 2960 3702 3787 4858 1391
Size: 38 34 153 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 322 287 153 104
85 84 74 38 34 31 24 19
BspEI T'CCGG_A
Cuts at: 2121 5304 2121
Size: 3183 2458
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 2996 844
Size: 1535 617 3489
Fragments arranged by size:
3489 1535 617
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2709 4136 4310 65
Size: 1183 1461 1427 174 1396
Fragments arranged by size:
1461 1427 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 2831 3701 4713 2831
Size: 870 1012 3759
Fragments arranged by size:
3759 1012 870
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2464 794
Size: 1670 3971
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636
Size: 999 367 385 17 1452 86 12 346
Cuts at: 5636 1972
Size: 1977
Fragments arranged by size:
1977 1452 999 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2090 2177 513
Size: 552 1025 87 3977
Fragments arranged by size:
3977 1025 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2353 2721 3157 3951 3971 2353
Size: 368 436 794 20 4023
Fragments arranged by size:
4023 794 436 368 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 2860 2925
Size: 54 1425 50 275 18 798 232 65
Cuts at: 2925 3088 3977 5606 8
Size: 163 889 1629 43
Fragments arranged by size:
1629 1425 889 798 275 232 163 65
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2925 3088 1537
Size: 275 1113 163 4090
Fragments arranged by size:
4090 1113 275 163
EarI CTCTTCn'nnn_
Cuts at: 1353 2122 3571 1353
Size: 769 1449 3423
Fragments arranged by size:
3423 1449 769
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2350 2404 2569 2822 3704 4301 5088
Size: 393 718 54 165 253 882 597 787
Cuts at: 5088 5356 1239
Size: 268 1524
Fragments arranged by size:
1524 882 787 718 597 393 268 253
165 54
EcoO109I rG'GnC_Cy
Cuts at: 2075 2476 2075
Size: 401 5240
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2454 2586 2645 4788 1678
Size: 776 132 59 2143 2531
Fragments arranged by size:
2531 2143 776 132 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2296 2985 3231 669
Size: 988 281 358 689 246 3079
Fragments arranged by size:
3079 988 689 358 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KasI G'GCGC_C
Cuts at: 2450 2450
Size: 5641
KpnI G_GTAC'C
Cuts at: 2395 2966 2395
Size: 571 5070
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 2604 2714 2726 3599 3958 4117
Size: 648 1192 246 110 12 873 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1192 873 648 359 246 159 110
12
MspA1I CmG'CkG
Cuts at: 1606 2092 2179 2290 3057 3734 4882 5127 1606
Size: 486 87 111 767 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 486 245 111 87
NarI GG'CG_CC
Cuts at: 2451 2451
Size: 5641
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2729 2999 4544 1132
Size: 97 1500 270 1545 2229
Fragments arranged by size:
2229 1545 1500 270 97
PasI CC'CwG_GG
Cuts at: 1348 2932 1348
Size: 1584 4057
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2075 2075
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2657 2657
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2140 2502 2595 2993 830
Size: 1310 362 93 398 3478
Fragments arranged by size:
3478 1310 398 362 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2290 2290
Size: 5641
SacII CC_GC'GG
Cuts at: 2093 2180 2093
Size: 87 5554
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SanDI GG'GwC_CC
Cuts at: 2075 2075
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SexAI A'CCwGG_T
Cuts at: 2394 2436 2394
Size: 42 5599
SfcI C'TryA_G
Cuts at: 826 1045 2136 2498 2591 2989 4124 4805 4996
Size: 219 1091 362 93 398 1135 681 191
Cuts at: 4996 826
Size: 1471
Fragments arranged by size:
1471 1135 1091 681 398 362 219 191
93
SfoI GGC'GCC
Cuts at: 2452 2452
Size: 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2080 2113 2968 1872
Size: 208 33 855 4545
Fragments arranged by size:
4545 855 208 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 2249 3745 4646 4908 820
Size: 197 926 95 211 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 262 211 197 95
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SrfI GCCC'GGGC
Cuts at: 2113 2113
Size: 5641
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2816 3080 513
Size: 552 1751 264 3074
Fragments arranged by size:
3074 1751 552 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3887 5599 96
Size: 274 80 33 51 1443 1910 1712 138
Fragments arranged by size:
1910 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 5601 5633 2835
Size: 32 2734 32 2843
Fragments arranged by size:
2843 2734 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2078 2111 2966 1870
Size: 208 33 855 4545
Fragments arranged by size:
4545 855 208 33
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SapI SfiI SwaI
XcmI