Restriction map for
pFN21AB6425
(Circular) (Six-base) MAPSORT of: pFN21AB6425.seq Check: 8952 from: 1 to: 5635
pFN21AB6425 5635 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2141 2141
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2118 3698 5082 5350 1632
Size: 486 1580 1384 268 1917
Fragments arranged by size:
1917 1580 1384 486 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 2215 3378 4950 2215
Size: 1163 1572 2900
Fragments arranged by size:
2900 1572 1163
ApaI G_GGCC'C
Cuts at: 2301 2301
Size: 5635
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2924 2944 3170 3322 5431 1493
Size: 1431 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 1431 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2352 2650 2960 1870
Size: 73 409 298 310 4545
Fragments arranged by size:
4545 409 310 298 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 4411 618
Size: 325 217 627 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2301 2448 2621 2796 2954 1391
Size: 910 147 173 175 158 4072
Fragments arranged by size:
4072 910 175 173 158 147
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BbvCI CC'TCA_GC
Cuts at: 2580 2580
Size: 5635
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2558 2592 3826 3860 1903
Size: 34 621 34 1234 34 3678
Fragments arranged by size:
3678 1234 621 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2435 2726 4246
Size: 107 122 71 631 605 763 291 1520
Cuts at: 4246 136
Size: 1525
Fragments arranged by size:
1525 1520 763 631 605 291 122 107
71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 2048 3052 2048
Size: 1004 4631
Bme1580I G_kGCm'C
Cuts at: 1790 2301 3696 4852 1790
Size: 511 1395 1156 2573
Fragments arranged by size:
2573 1395 1156 511
BmgBI CAC'GTC
Cuts at: 1318 2232 1318
Size: 914 4721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2699 3366 4324
Size: 416 53 83 399 24 1282 667 958
Cuts at: 4324 442
Size: 1753
Fragments arranged by size:
1753 1282 958 667 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2310 2578 4295 1239
Size: 981 90 268 1717 2579
Fragments arranged by size:
2579 1717 981 268 90
Bpu10I CC'TnA_GC
Cuts at: 2143 2580 2143
Size: 437 5198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2378 2486 4298 882
Size: 617 879 108 1812 2219
Fragments arranged by size:
2219 1812 879 617 108
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 2301 2331 2724
Size: 30 165 30 717 30 151 30 393
Cuts at: 2724 2754 2772 2802 1178
Size: 30 18 30 4011
Fragments arranged by size:
4011 717 393 165 151 30 30 30
30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2885 1186
Size: 1699 3936
BseYI C'CCAG_C
Cuts at: 2513 2605 2761 4838 5334 2513
Size: 92 156 2077 496 2814
Fragments arranged by size:
2814 2077 496 156 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2169 1163
Size: 573 433 4629
Fragments arranged by size:
4629 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3696 3781 4852 3696
Size: 85 1071 4479
Fragments arranged by size:
4479 1071 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
BsmBI CGTCTCn'nnnn_
Cuts at: 2238 2238
Size: 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2448 2621 2796 2954 3696 3781
Size: 399 511 147 173 175 158 742 85
Cuts at: 3781 4852 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 511 399 175 173 158
147 85
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 2990 844
Size: 1297 849 3489
Fragments arranged by size:
3489 1297 849
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2772 4130 4304 65
Size: 1183 1524 1358 174 1396
Fragments arranged by size:
1524 1396 1358 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2246 2284 4279 4466 1700
Size: 110 249 187 38 1995 187 2869
Fragments arranged by size:
2869 1995 249 187 187 110 38
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 2011 2037 1925
Size: 86 26 5523
Fragments arranged by size:
5523 86 26
BssSI C'ACGA_G
Cuts at: 2786 3695 4707 2786
Size: 909 1012 3714
Fragments arranged by size:
3714 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 2251 2251
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630
Size: 993 367 385 17 1452 86 12 346
Cuts at: 5630 1972
Size: 1977
Fragments arranged by size:
1977 1452 993 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2433 2676 2721 513
Size: 552 1368 243 45 3427
Fragments arranged by size:
3427 1368 552 243 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 2673 3082
Size: 54 1425 50 275 18 652 191 409
Cuts at: 3082 3971 5600 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 652 409 275 191 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3565 1353
Size: 2212 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2220 2310 2578 3698 4295 5082
Size: 393 486 102 90 268 1120 597 787
Cuts at: 5082 5350 1239
Size: 268 1524
Fragments arranged by size:
1524 1120 787 597 486 393 268 268
102 90
EcoO109I rG'GnC_Cy
Cuts at: 2297 2510 2555 2727 2737 2895 2297
Size: 213 45 172 10 158 5037
Fragments arranged by size:
5037 213 172 158 45 10
EcoRI G'AATT_C
Cuts at: 2924 2944 3322 2924
Size: 20 378 5237
Fragments arranged by size:
5237 378 20
EcoRV GAT'ATC
Cuts at: 2167 2167
Size: 5635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2543 2543
Size: 5635
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2021 2048 4782 1678
Size: 343 27 2734 2531
Fragments arranged by size:
2734 2531 343 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2392 2424 2576
Size: 32 135 32 548 32 240 32 152
Cuts at: 2576 2608 4276 4308 4350 4382 1373
Size: 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 548 240 152 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2484 10
Size: 54 1425 343 652 3161
Fragments arranged by size:
3161 1425 652 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3593 3952 4111 518
Size: 648 2427 359 159 2042
Fragments arranged by size:
2427 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2264 3051 3728 4876 5121 1606
Size: 658 787 677 1148 245 2120
Fragments arranged by size:
2120 1148 787 677 658 245
NaeI GCC'GGC
Cuts at: 2061 2286 2061
Size: 225 5410
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5083
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 2059 2284 2059
Size: 225 5410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2476 2993 4538 1132
Size: 97 1247 517 1545 2229
Fragments arranged by size:
2229 1545 1247 517 97
PasI CC'CwG_GG
Cuts at: 1348 2379 1348
Size: 1031 4604
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PfoI T'CCnGG_A
Cuts at: 2742 2742
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2510 2555 2510
Size: 45 5590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PspOMI G'GGCC_C
Cuts at: 2297 2297
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2058 2509 2987 830
Size: 1228 451 478 3478
Fragments arranged by size:
3478 1228 478 451
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2264 2264
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SbfI CC_TGCA'GG
Cuts at: 2509 2987 2509
Size: 478 5157
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2054 2259 2316 2505 2667 2983 4118
Size: 219 1009 205 57 189 162 316 1135
Cuts at: 4118 4799 4990 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 316 219 205 191
189 162 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 2726 2726
Size: 5635
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2354 2962 1872
Size: 482 608 4545
Fragments arranged by size:
4545 608 482
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 2414 2784 1517
Size: 897 370 4368
Fragments arranged by size:
4368 897 370
StyI C'CwwG_G
Cuts at: 513 1065 2301 2865 3074 513
Size: 552 1236 564 209 3074
Fragments arranged by size:
3074 1236 564 552 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2724 2756 2772 2804 5595 5627 2724
Size: 32 16 32 2791 32 2732
Fragments arranged by size:
2791 2732 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2352 2960 1870
Size: 482 608 4545
Fragments arranged by size:
4545 608 482
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfoI SrfI
SwaI