Restriction map for
pFN21AB6422
(Circular) (Six-base) MAPSORT of: pFN21AB6422.seq Check: 9938 from: 1 to: 5722
pFN21AB6422 5722 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2441 2441
Size: 5722
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2667 3065 1937
Size: 730 398 4594
Fragments arranged by size:
4594 730 398
Acc65I G'GTAC_C
Cuts at: 2495 3043 2495
Size: 548 5174
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2028 2988 2994 3785 5169 5437 1632
Size: 396 960 6 791 1384 268 1917
Fragments arranged by size:
1917 1384 960 791 396 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 2528 2937 3465 5037 2528
Size: 409 528 1572 3213
Fragments arranged by size:
3213 1572 528 409
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2223 2276 2387 3031 3257 3409 5518 1493
Size: 730 53 111 644 226 152 2109 1697
Fragments arranged by size:
2109 1697 730 644 226 152 111 53
AseI AT'TA_AT
Cuts at: 160 1032 2991 4276 160
Size: 872 1959 1285 1606
Fragments arranged by size:
1959 1606 1285 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 2580 2701 3047 1870
Size: 73 637 121 346 4545
Fragments arranged by size:
4545 637 346 121 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2031 2064 2031
Size: 33 5689
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 3043 4498 618
Size: 325 217 627 708 548 1455 1842
Fragments arranged by size:
1842 1455 708 627 548 325 217
BanII G_rGCy'C
Cuts at: 1391 3041 1391
Size: 1650 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2408 2954 928
Size: 971 509 546 3696
Fragments arranged by size:
3696 971 546 509
BbvCI CC'TCA_GC
Cuts at: 2232 2232
Size: 5722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BfrBI ATG'CAT
Cuts at: 2888 2888
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2512 4333 136
Size: 107 122 71 631 605 840 1821 1525
Fragments arranged by size:
1821 1525 840 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2409 5717 2409
Size: 3308 2414
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 3783 4939 1790
Size: 1993 1156 2573
Fragments arranged by size:
2573 1993 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2406 3453 4411
Size: 416 53 83 399 24 989 1047 958
Cuts at: 4411 442
Size: 1753
Fragments arranged by size:
1753 1047 989 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2426 1056
Size: 1370 4352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4382 1239
Size: 3143 2579
Bpu10I CC'TnA_GC
Cuts at: 2232 2232
Size: 5722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2802 3847 4712 5010 2802
Size: 1045 865 298 3514
Fragments arranged by size:
3514 1045 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2546 4385 882
Size: 617 1047 1839 2219
Fragments arranged by size:
2219 1839 1047 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 5716 5716
Size: 5722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4155 4553 4827 4974 5385 4155
Size: 398 274 147 411 4492
Fragments arranged by size:
4492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5497
Fragments arranged by size:
5497 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5722
BseYI C'CCAG_C
Cuts at: 2607 4925 5421 2607
Size: 2318 496 2908
Fragments arranged by size:
2908 2318 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3783 3868 4939 3783
Size: 85 1071 4566
Fragments arranged by size:
4566 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3041 3783 3868 4939 1391
Size: 399 1251 742 85 1071 2174
Fragments arranged by size:
2174 1251 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2441 3077 844
Size: 1597 636 3489
Fragments arranged by size:
3489 1597 636
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 4217 4391 65
Size: 1183 768 2201 174 1396
Fragments arranged by size:
2201 1396 1183 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4366 4553 1700
Size: 110 2556 187 2869
Fragments arranged by size:
2869 2556 187 110
BsrGI T'GTAC_A
Cuts at: 96 2478 96
Size: 2382 3340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2363 2409 3052 3419 3804 3821 5273 5359
Size: 391 46 643 367 385 17 1452 86
Cuts at: 5359 5371 5717 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 643 391 385 367 346 86
46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4231
Fragments arranged by size:
4231 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2849 2863 487
Size: 683 56 1623 14 3346
Fragments arranged by size:
3346 1623 683 56 14
BtsI GCAGTG_nn'
Cuts at: 2099 2265 2529 2557 2654 2796 3238 4032 4052
Size: 166 264 28 97 142 442 794 20
Cuts at: 4052 2099
Size: 3769
Fragments arranged by size:
3769 794 442 264 166 142 97 28
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2739 3169 4058
Size: 54 1425 50 275 18 909 430 889
Cuts at: 4058 5687 8
Size: 1629 43
Fragments arranged by size:
1629 1425 909 889 430 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2914 3652 1353
Size: 1561 738 3423
Fragments arranged by size:
3423 1561 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2988 2994 3785 4382 5169 5437
Size: 393 396 960 6 791 597 787 268
Cuts at: 5437 1239
Size: 1524
Fragments arranged by size:
1524 960 791 787 597 396 393 268
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2865 2865
Size: 5722
EcoRI G'AATT_C
Cuts at: 2276 2387 3031 3409 2276
Size: 111 644 378 4589
Fragments arranged by size:
4589 644 378 111
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 4869 1678
Size: 3191 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2524 2556 2859
Size: 32 135 32 776 32 144 32 303
Cuts at: 2859 2891 4363 4395 4437 4469 1373
Size: 32 1472 32 42 32 2626
Fragments arranged by size:
2626 1472 776 303 144 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3312 669
Size: 988 281 1128 246 3079
Fragments arranged by size:
3079 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2059 748
Size: 1311 4411
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 2499 3047 2499
Size: 548 5174
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4836 5020 1305
Size: 3531 184 2007
Fragments arranged by size:
3531 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2741 10
Size: 54 1425 343 909 2991
Fragments arranged by size:
2991 1425 909 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2375 3680 4039 4198 518
Size: 648 843 366 1305 359 159 2042
Fragments arranged by size:
2042 1305 843 648 366 359 159
MspA1I CmG'CkG
Cuts at: 1606 3138 3815 4963 5208 1606
Size: 1532 677 1148 245 2120
Fragments arranged by size:
2120 1532 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4231
Fragments arranged by size:
4231 939 552
NdeI CA'TA_TG
Cuts at: 387 2575 387
Size: 2188 3534
NheI G'CTAG_C
Cuts at: 1052 2422 1052
Size: 1370 4352
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NsiI A_TGCA'T
Cuts at: 2890 2890
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 3080 4625 1132
Size: 97 1851 1545 2229
Fragments arranged by size:
2229 1851 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 1108
Size: 1041 4681
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 5722
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PspXI vC'TCGA_Gb
Cuts at: 2580 2580
Size: 5722
PstI C_TGCA'G
Cuts at: 830 3074 830
Size: 2244 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 3070 4205 4886 5077 826
Size: 219 2025 1135 681 191 1471
Fragments arranged by size:
2025 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2580 2817 3826 4727 4989 820
Size: 197 926 637 237 1009 901 262 1553
Fragments arranged by size:
1553 1009 926 901 637 262 237 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 2191 3646 5
Size: 47 2139 1455 2081
Fragments arranged by size:
2139 2081 1455 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 2004 3161 513
Size: 552 939 1157 3074
Fragments arranged by size:
3074 1157 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3749 3766 3919 4104 4549 325
Size: 1255 2169 17 153 185 445 1498
Fragments arranged by size:
2169 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 3968 5680
Size: 274 80 33 51 1443 501 1490 1712
Cuts at: 5680 96
Size: 138
Fragments arranged by size:
1712 1490 1443 501 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 2580 1943
Size: 637 5085
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 3851
Size: 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI