Restriction map for
pFN21AB6421
(Circular) (Six-base) MAPSORT of: pFN21AB6421.seq Check: 5253 from: 1 to: 5632
pFN21AB6421 5632 bp
With 182 enzymes: SgfI *
October 20, 2009 11:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2883 3695 5079 5347 1632
Size: 1251 812 1384 268 1917
Fragments arranged by size:
1917 1384 1251 812 268
AflII C'TTAA_G
Cuts at: 820 1017 2540 820
Size: 197 1523 3912
Fragments arranged by size:
3912 1523 197
AflIII A'CryG_T
Cuts at: 1203 2651 4531 5508 1203
Size: 1448 1880 977 1327
Fragments arranged by size:
1880 1448 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 2280 1184
Size: 915 181 4536
Fragments arranged by size:
4536 915 181
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2265 2297 2265
Size: 32 5600
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2314 2432 2600 2904 2941 3167 3319 5428
Size: 821 118 168 304 37 226 152 2109
Cuts at: 5428 1493
Size: 1697
Fragments arranged by size:
2109 1697 821 304 226 168 152 118
37
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 2166 2957 1870
Size: 73 186 37 791 4545
Fragments arranged by size:
4545 791 186 73 37
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2953 4408 618
Size: 325 217 627 248 918 1455 1842
Fragments arranged by size:
1842 1455 918 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2951 1391
Size: 1560 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 928
Size: 971 506 4155
Fragments arranged by size:
4155 971 506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2100 2134 3823 3857 1903
Size: 34 163 34 1689 34 3678
Fragments arranged by size:
3678 1689 163 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2539 3693 4849 1790
Size: 749 1154 1156 2573
Fragments arranged by size:
2573 1156 1154 749
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4292 1239
Size: 3053 2579
Bpu10I CC'TnA_GC
Cuts at: 2175 2370 2175
Size: 195 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 1186
Size: 961 4671
BseYI C'CCAG_C
Cuts at: 2058 4835 5331 2058
Size: 2777 496 2359
Fragments arranged by size:
2777 2359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2120 3082 3843 4871 1540
Size: 275 186 119 962 761 1028 2301
Fragments arranged by size:
2301 1028 962 761 275 186 119
BsiHKAI G_wGCw'C
Cuts at: 2718 3693 3778 4849 2718
Size: 975 85 1071 3501
Fragments arranged by size:
3501 1071 975 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2539 2718 2951 3693 3778 4849 1391
Size: 399 749 179 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 749 742 399 233 179 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2357 794
Size: 1563 4069
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2753 2866 2962 3329 3714 3731 5183 5269
Size: 781 113 96 367 385 17 1452 86
Cuts at: 5269 5281 5627 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 781 385 367 346 113 96
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2184 513
Size: 552 939 39 141 3961
Fragments arranged by size:
3961 939 552 141 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2358 3148 3942 3962 2118
Size: 240 790 794 20 3788
Fragments arranged by size:
3788 794 790 240 20
ClaI AT'CG_AT
Cuts at: 1554 2120 1554
Size: 566 5066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2149 3079 3968
Size: 54 1425 50 275 18 319 930 889
Cuts at: 3968 5597 8
Size: 1629 43
Fragments arranged by size:
1629 1425 930 889 319 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2883 3695 4292 5079 5347 1239
Size: 393 1251 812 597 787 268 1524
Fragments arranged by size:
1524 1251 812 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2432 2941 3319 2432
Size: 509 378 4745
Fragments arranged by size:
4745 509 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 2822 2854 4273
Size: 32 135 32 1187 32 31 32 1419
Cuts at: 4273 4305 4347 4379 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1419 1187 135 42 32 32 32
32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2488 2548 748
Size: 1740 60 3832
Fragments arranged by size:
3832 1740 60
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2362 2551 4746 4930 1305
Size: 1057 189 2195 184 2007
Fragments arranged by size:
2195 2007 1057 189 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2151 10
Size: 54 1425 343 319 3491
Fragments arranged by size:
3491 1425 343 319 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 3590 3949 4108 518
Size: 648 1600 824 359 159 2042
Fragments arranged by size:
2042 1600 824 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2512 2593 3048 3725 4873 5118 1606
Size: 906 81 455 677 1148 245 2120
Fragments arranged by size:
2120 1148 906 677 455 245 81
NcoI C'CATG_G
Cuts at: 513 1065 2004 2043 2184 513
Size: 552 939 39 141 3961
Fragments arranged by size:
3961 939 552 141 39
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2417 1108
Size: 1309 4323
PfoI T'CCnGG_A
Cuts at: 2661 2661
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 2643 3202 2643
Size: 559 5073
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2120 2001
Size: 119 5513
PvuII CAG'CTG
Cuts at: 2512 2512
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2980 4115 4796 4987 826
Size: 219 1935 1135 681 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2168 2959 1872
Size: 296 791 4545
Fragments arranged by size:
4545 791 296
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 3736 4637 4899 820
Size: 197 926 597 1196 901 262 1553
Fragments arranged by size:
1553 1196 926 901 597 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 2402 152
Size: 2250 3382
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2184 2237 3071 513
Size: 552 939 39 141 53 834 3074
Fragments arranged by size:
3074 939 834 552 141 53 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 5590 96
Size: 274 80 33 51 1443 1901 1712 138
Fragments arranged by size:
1901 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2643 2675 5592 5624 2643
Size: 32 2917 32 2651
Fragments arranged by size:
2917 2651 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2756 2968 2756
Size: 212 5420
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2166 2957 1870
Size: 296 791 4545
Fragments arranged by size:
4545 791 296
XmnI GAAnn'nnTTC
Cuts at: 2436 2904 3761 2436
Size: 468 857 4307
Fragments arranged by size:
4307 857 468
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI