Restriction map for
pFN21AB6407
(Circular) (Six-base) MAPSORT of: pFN21AB6407.seq Check: 1492 from: 1 to: 5623
pFN21AB6407 5623 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2511 3433 278
Size: 53 83 186 936 975 922 2468
Fragments arranged by size:
2468 975 936 922 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2966 1937
Size: 1029 4594
Acc65I G'GTAC_C
Cuts at: 2944 2944
Size: 5623
AclI AA'CG_TT
Cuts at: 1563 3750 4123 1563
Size: 2187 373 3063
Fragments arranged by size:
3063 2187 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 3686 5070 5338 1632
Size: 1265 789 1384 268 1917
Fragments arranged by size:
1917 1384 1265 789 268
AfeI AGC'GCT
Cuts at: 2711 2711
Size: 5623
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5426
AflIII A'CryG_T
Cuts at: 1203 4522 5499 1203
Size: 3319 977 1327
Fragments arranged by size:
3319 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 4352 4352
Size: 5623
AleI CACnn'nnGTG
Cuts at: 1166 2783 1166
Size: 1617 4006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2801 1184
Size: 1617 4006
AlwNI CAG_nnn'CTG
Cuts at: 3366 4938 3366
Size: 1572 4051
ApaLI G'TGCA_C
Cuts at: 3680 4836 3680
Size: 1156 4467
ApoI r'AATT_y
Cuts at: 1493 2135 2932 3158 3310 5419 1493
Size: 642 797 226 152 2109 1697
Fragments arranged by size:
2109 1697 797 642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4177 160
Size: 872 3145 1606
Fragments arranged by size:
3145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2643 2948 1870
Size: 73 123 577 305 4545
Fragments arranged by size:
4545 577 305 123 73
BamHI G'GATC_C
Cuts at: 2049 2953 3320 2049
Size: 904 367 4352
Fragments arranged by size:
4352 904 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 2944 4399 618
Size: 325 217 627 998 159 1455 1842
Fragments arranged by size:
1842 1455 998 627 325 217 159
BanII G_rGCy'C
Cuts at: 1391 2942 1391
Size: 1551 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2047 2081 3814 3848 1903
Size: 34 110 34 1733 34 3678
Fragments arranged by size:
3678 1733 110 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3515 4725 3515
Size: 1210 4413
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4234 136
Size: 107 122 71 631 605 2562 1525
Fragments arranged by size:
2562 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2293 2912 5618 2293
Size: 619 2706 2298
Fragments arranged by size:
2706 2298 619
BlpI GC'TnA_GC
Cuts at: 2546 2774 3040 2546
Size: 228 266 5129
Fragments arranged by size:
5129 266 228
Bme1580I G_kGCm'C
Cuts at: 1790 2790 3684 4840 1790
Size: 1000 894 1156 2573
Fragments arranged by size:
2573 1156 1000 894
BmgBI CAC'GTC
Cuts at: 1318 2570 1318
Size: 1252 4371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2400 3354 4312
Size: 416 53 83 399 24 983 954 958
Cuts at: 4312 442
Size: 1753
Fragments arranged by size:
1753 983 958 954 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2546 2546
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2309 2726 4283 1239
Size: 911 159 417 1557 2579
Fragments arranged by size:
2579 1557 911 417 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2318 3748 4613 4911 2318
Size: 1430 865 298 3030
Fragments arranged by size:
3030 1430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4286 882
Size: 617 2787 2219
Fragments arranged by size:
2787 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2044 2580 3414 493
Size: 711 840 536 834 2702
Fragments arranged by size:
2702 840 834 711 536
BsaBI GATnn'nnATC
Cuts at: 5617 5617
Size: 5623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2062 2508 3430 3812
Size: 53 83 186 936 529 446 922 382
Cuts at: 3812 4447 275
Size: 635 1451
Fragments arranged by size:
1451 936 922 635 529 446 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2052 4056 4454 4728 4875 5286 2052
Size: 2004 398 274 147 411 2389
Fragments arranged by size:
2389 2004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2252 2282 2593 2623 1178
Size: 30 165 30 849 30 311 30 4178
Fragments arranged by size:
4178 849 311 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5623
BseYI C'CCAG_C
Cuts at: 4826 5322 4826
Size: 496 5127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5050
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3834 4862 1540
Size: 275 186 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2780 2922 3684 3769 4840 2780
Size: 142 762 85 1071 3563
Fragments arranged by size:
3563 1071 762 142 85
BsmI GAATG_Cn'
Cuts at: 3134 3227 3134
Size: 93 5530
BsmBI CGTCTCn'nnnn_
Cuts at: 2640 2640
Size: 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2780 2790 2922 2942 3684 3769 4840
Size: 399 990 10 132 20 742 85 1071
Cuts at: 4840 1391
Size: 2174
Fragments arranged by size:
2174 1071 990 742 399 132 85 20
10
BspEI T'CCGG_A
Cuts at: 2052 5286 2052
Size: 3234 2389
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2978 844
Size: 2134 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2761 3510 1394
Size: 429 938 749 3507
Fragments arranged by size:
3507 938 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4118 4292 65
Size: 1183 2870 174 1396
Fragments arranged by size:
2870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4267 4454 1700
Size: 110 2457 187 2869
Fragments arranged by size:
2869 2457 187 110
BsrGI T'GTAC_A
Cuts at: 96 2455 96
Size: 2359 3264
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5623
BssSI C'ACGA_G
Cuts at: 2705 3683 4695 2705
Size: 978 1012 3633
Fragments arranged by size:
3633 1012 978
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5623
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5623
BstYI r'GATC_y
Cuts at: 1972 2049 2293 2912 2953 3320 3705 3722 5174
Size: 77 244 619 41 367 385 17 1452
Cuts at: 5174 5260 5272 5618 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 619 385 367 346 244 86
77 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3355
Fragments arranged by size:
3355 939 777 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2095 487
Size: 683 56 869 4015
Fragments arranged by size:
4015 869 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 3933 3953 3139
Size: 794 20 4809
Fragments arranged by size:
4809 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2377 2412 529
Size: 35 1813 35 3740
Fragments arranged by size:
3740 1813 35 35
DraI TTT'AAA
Cuts at: 2927 2927
Size: 5623
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2540 2688
Size: 54 1425 50 275 18 555 155 148
Cuts at: 2688 3070 3959 5588 8
Size: 382 889 1629 43
Fragments arranged by size:
1629 1425 889 555 382 275 155 148
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3553 1353
Size: 2200 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4210 4584 4730 387
Size: 1064 138 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2309 2726 2897 3686 4283 5070
Size: 393 518 159 417 171 789 597 787
Cuts at: 5070 5338 1239
Size: 268 1524
Fragments arranged by size:
1524 789 787 597 518 417 393 268
171 159
EcoRI G'AATT_C
Cuts at: 2932 3310 2932
Size: 378 5245
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 2553 2713 2805 4770 1678
Size: 875 160 92 1965 2531
Fragments arranged by size:
2531 1965 875 160 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2425 2457 4264
Size: 32 135 32 680 32 141 32 1807
Cuts at: 4264 4296 4338 4370 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1807 680 141 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2967 3213 669
Size: 988 281 1029 246 3079
Fragments arranged by size:
3079 1029 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2103 2879 748
Size: 1355 776 3492
Fragments arranged by size:
3492 1355 776
HpaI GTT'AAC
Cuts at: 3213 3213
Size: 5623
KpnI G_GTAC'C
Cuts at: 2948 2948
Size: 5623
MfeI C'AATT_G
Cuts at: 3222 3222
Size: 5623
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4737 4921 1305
Size: 3432 184 2007
Fragments arranged by size:
3432 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 2690 10
Size: 54 1425 343 555 303 2943
Fragments arranged by size:
2943 1425 555 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2783 3581 3940 4099 518
Size: 648 1617 798 359 159 2042
Fragments arranged by size:
2042 1617 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3039 3716 4864 5109 1606
Size: 1433 677 1148 245 2120
Fragments arranged by size:
2120 1433 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3355
Fragments arranged by size:
3355 939 777 552
NdeI CA'TA_TG
Cuts at: 387 2485 387
Size: 2098 3525
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2240 2981 4526 1132
Size: 97 1011 741 1545 2229
Fragments arranged by size:
2229 1545 1011 741 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5623
PciI A'CATG_T
Cuts at: 4522 4522
Size: 5623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5623
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 5205 5237 3692
Size: 32 1481 32 4078
Fragments arranged by size:
4078 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5623
PsiI TTA'TAA
Cuts at: 3193 3193
Size: 5623
PstI C_TGCA'G
Cuts at: 830 2975 830
Size: 2145 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5623
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SbfI CC_TGCA'GG
Cuts at: 2975 2975
Size: 5623
ScaI AGT'ACT
Cuts at: 2322 3871 5583 2322
Size: 1549 1712 2362
Fragments arranged by size:
2362 1712 1549
SfcI C'TryA_G
Cuts at: 826 1045 2717 2908 2971 4106 4787 4978 826
Size: 219 1672 191 63 1135 681 191 1471
Fragments arranged by size:
1672 1471 1135 681 219 191 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5623
SmaI CCC'GGG
Cuts at: 1872 2950 1872
Size: 1078 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 3727 4628 4890 820
Size: 197 926 354 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 354 262 197
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2981 1132
Size: 1849 3774
SspI AAT'ATT
Cuts at: 5 52 3547 5
Size: 47 3495 2081
Fragments arranged by size:
3495 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5623
StyI C'CwwG_G
Cuts at: 513 1065 2004 2365 2685 2781 3062 513
Size: 552 939 361 320 96 281 3074
Fragments arranged by size:
3074 939 552 361 320 281 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3650 3667 3820 4005 4450 325
Size: 1255 2070 17 153 185 445 1498
Fragments arranged by size:
2070 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2320 2455 3869
Size: 274 80 33 51 1443 343 135 1414
Cuts at: 3869 5581 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1414 343 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 5583 5615 2593
Size: 32 2958 32 2601
Fragments arranged by size:
2958 2601 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5623
XbaI T'CTAG_A
Cuts at: 2959 2959
Size: 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5623
XmaI C'CCGG_G
Cuts at: 1870 2948 1870
Size: 1078 4545
XmnI GAAnn'nnTTC
Cuts at: 3752 3752
Size: 5623
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2509 3431 276
Size: 53 83 186 936 975 922 2468
Fragments arranged by size:
2468 975 936 922 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI