Restriction map for
pFN21AB6406
(Circular) (Six-base) MAPSORT of: pFN21AB6406.seq Check: 6734 from: 1 to: 5710
pFN21AB6406 5710 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2196 2592 3773 5157 5425 1632
Size: 564 396 1181 1384 268 1917
Fragments arranged by size:
1917 1384 1181 564 396 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 2135 3453 5025 2135
Size: 1318 1572 2820
Fragments arranged by size:
2820 1572 1318
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2451 3019 3245 3397 5506 1493
Size: 958 568 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2307 3035 1870
Size: 73 364 728 4545
Fragments arranged by size:
4545 728 364 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 3031 4486 618
Size: 325 217 627 233 1011 1455 1842
Fragments arranged by size:
1842 1455 1011 627 325 233 217
BanII G_rGCy'C
Cuts at: 1391 2857 3029 1391
Size: 1466 172 4072
Fragments arranged by size:
4072 1466 172
BbeI G_GCGC'C
Cuts at: 2024 2024
Size: 5710
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2374 5705 2374
Size: 3331 2379
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4927 1790
Size: 1981 1156 2573
Fragments arranged by size:
2573 1981 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2724 2832 3441
Size: 416 53 83 399 24 1307 108 609
Cuts at: 3441 4399 442
Size: 958 1753
Fragments arranged by size:
1753 1307 958 609 416 399 108 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 2937 4370 1239
Size: 975 723 1433 2579
Fragments arranged by size:
2579 1433 975 723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2252 3501 493
Size: 711 1048 1249 2702
Fragments arranged by size:
2702 1249 1048 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3517 3899 4534
Size: 53 83 186 936 488 1496 382 635
Cuts at: 4534 275
Size: 1451
Fragments arranged by size:
1496 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3000 4143 4541 4815 4962 5373 3000
Size: 1143 398 274 147 411 3337
Fragments arranged by size:
3337 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5710
BseYI C'CCAG_C
Cuts at: 2597 4913 5409 2597
Size: 2316 496 2898
Fragments arranged by size:
2898 2316 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2577 1163
Size: 573 841 4296
Fragments arranged by size:
4296 841 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2857 3029 3771 3856 4927 1391
Size: 399 1067 172 742 85 1071 2174
Fragments arranged by size:
2174 1071 1067 742 399 172 85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3065 844
Size: 2221 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 4205 4379 65
Size: 1183 1275 1682 174 1396
Fragments arranged by size:
1682 1396 1275 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 2457 3770 4782 2457
Size: 1313 1012 3385
Fragments arranged by size:
3385 1313 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2374 3040 3407 3792 3809 5261 5347 5359
Size: 402 666 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 666 402 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675
Size: 54 1425 50 275 18 1327 889 1629
Cuts at: 5675 8
Size: 43
Fragments arranged by size:
1629 1425 1327 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 2545 3640 1353
Size: 1104 88 1095 3423
Fragments arranged by size:
3423 1104 1095 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2214 2592 2937 3773 4370 5157
Size: 393 564 18 378 345 836 597 787
Cuts at: 5157 5425 1239
Size: 268 1524
Fragments arranged by size:
1524 836 787 597 564 393 378 345
268 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2196 2690 2867 2196
Size: 494 177 5039
Fragments arranged by size:
5039 494 177
EcoRI G'AATT_C
Cuts at: 2451 3019 3397 2451
Size: 568 378 4764
Fragments arranged by size:
4764 568 378
EcoRV GAT'ATC
Cuts at: 2773 2773
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2024 4857 1678
Size: 346 2833 2531
Fragments arranged by size:
2833 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 4351 4383 4425
Size: 32 135 32 1100 32 1647 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 1647 1100 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KasI G'GCGC_C
Cuts at: 2020 2020
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 3668 4027 4186 518
Size: 648 888 1614 359 159 2042
Fragments arranged by size:
2042 1614 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3126 3803 4951 5196 1606
Size: 1520 677 1148 245 2120
Fragments arranged by size:
2120 1520 1148 677 245
NarI GG'CG_CC
Cuts at: 2021 2021
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5158
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2059 3068 4613 1132
Size: 97 830 1009 1545 2229
Fragments arranged by size:
2229 1545 1009 830 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 3779 3811 5292 5324 2690
Size: 32 1057 32 1481 32 3076
Fragments arranged by size:
3076 1481 1057 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspXI vC'TCGA_Gb
Cuts at: 2307 2307
Size: 5710
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2529 3058 4193 4874 5065 826
Size: 219 1484 529 1135 681 191 1471
Fragments arranged by size:
1484 1471 1135 681 529 219 191
SfoI GGC'GCC
Cuts at: 2022 2022
Size: 5710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 3814 4715 4977 820
Size: 197 926 364 1507 901 262 1553
Fragments arranged by size:
1553 1507 926 901 364 262 197
SnaBI TAC'GTA
Cuts at: 493 2252 3501 493
Size: 1759 1249 2702
Fragments arranged by size:
2702 1759 1249
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 2429 3634 5
Size: 47 2377 1205 2081
Fragments arranged by size:
2377 2081 1205 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2904 2994 3149 513
Size: 552 1839 90 155 3074
Fragments arranged by size:
3074 1839 552 155 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 3737 3754 3907 4092 4537 325
Size: 1255 1122 1035 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1122 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 2488 2889
Size: 274 80 33 51 1443 132 379 401
Cuts at: 2889 3956 5668 96
Size: 1067 1712 138
Fragments arranged by size:
1712 1443 1067 401 379 274 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2884 2916 5670 5702 2884
Size: 32 2754 32 2892
Fragments arranged by size:
2892 2754 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 2902 2572
Size: 330 5380
XhoI C'TCGA_G
Cuts at: 1943 2307 1943
Size: 364 5346
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 2265 3839 2265
Size: 1574 4136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI