Restriction map for
pFN21AB6395
(Circular) (Six-base) MAPSORT of: pFN21AB6395.seq Check: 1878 from: 1 to: 5533
pFN21AB6395 5533 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 2196 3660 4033 1563
Size: 633 1464 373 3063
Fragments arranged by size:
3063 1464 633 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 3596 4980 5248 1632
Size: 1056 908 1384 268 1917
Fragments arranged by size:
1917 1384 1056 908 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 4432 5409 1203
Size: 3229 977 1327
Fragments arranged by size:
3229 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 2173 3276 4848 2173
Size: 1103 1572 2858
Fragments arranged by size:
2858 1572 1103
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2159 2842 3068 3220 5329 1493
Size: 666 683 226 152 2109 1697
Fragments arranged by size:
2109 1697 683 666 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2139 2274 2858 1870
Size: 73 91 105 135 584 4545
Fragments arranged by size:
4545 584 135 105 91 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 2348 2854 4309 618
Size: 325 217 627 432 129 506 1455 1842
Fragments arranged by size:
1842 1455 627 506 432 325 217 129
BanII G_rGCy'C
Cuts at: 1391 2032 2130 2852 1391
Size: 641 98 722 4072
Fragments arranged by size:
4072 722 641 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4562
BbvCI CC'TCA_GC
Cuts at: 2281 2281
Size: 5533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4144 136
Size: 107 122 71 631 605 2472 1525
Fragments arranged by size:
2472 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2950 2950
Size: 5533
Bme1580I G_kGCm'C
Cuts at: 1790 3594 4750 1790
Size: 1804 1156 2573
Fragments arranged by size:
2573 1804 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 3264 4222
Size: 416 53 83 399 24 975 872 958
Cuts at: 4222 442
Size: 1753
Fragments arranged by size:
1753 975 958 872 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2106 2751 4193 1239
Size: 867 645 1442 2579
Fragments arranged by size:
2579 1442 867 645
Bpu10I CC'TnA_GC
Cuts at: 2281 2281
Size: 5533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4196 882
Size: 617 2697 2219
Fragments arranged by size:
2697 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 3324 493
Size: 711 1356 764 2702
Fragments arranged by size:
2702 1356 764 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2080 3340 3722 4357
Size: 53 83 186 936 547 1260 382 635
Cuts at: 4357 275
Size: 1451
Fragments arranged by size:
1451 1260 936 635 547 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2724 2754 1178
Size: 30 165 30 1321 30 3957
Fragments arranged by size:
3957 1321 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5533
BseYI C'CCAG_C
Cuts at: 2285 2529 4736 5232 2285
Size: 244 2207 496 2586
Fragments arranged by size:
2586 2207 496 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2385 2792 1163
Size: 573 649 407 3904
Fragments arranged by size:
3904 649 573 407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 2983 3744 4772 1540
Size: 275 186 47 935 761 1028 2301
Fragments arranged by size:
2301 1028 935 761 275 186 47
BsiHKAI G_wGCw'C
Cuts at: 2130 3594 3679 4750 2130
Size: 1464 85 1071 2913
Fragments arranged by size:
2913 1464 1071 85
BsmI GAATG_Cn'
Cuts at: 2151 2411 3044 3137 2151
Size: 260 633 93 4547
Fragments arranged by size:
4547 633 260 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2130 2852 3594 3679 4750 1391
Size: 399 242 98 722 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 722 399 242 98 85
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 3422 5152 3422
Size: 1730 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2349 2811 2888 844
Size: 1505 462 77 3489
Fragments arranged by size:
3489 1505 462 77
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528
Size: 891 367 385 17 1452 86 12 346
Cuts at: 5528 1972
Size: 1977
Fragments arranged by size:
1977 1452 891 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2215 2601 513
Size: 552 939 211 386 3445
Fragments arranged by size:
3445 939 552 386 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 3049 3843 3863 2271
Size: 778 794 20 3941
Fragments arranged by size:
3941 794 778 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 2397 2686
Size: 54 1425 50 275 18 215 352 289
Cuts at: 2686 2980 3869 5498 8
Size: 294 889 1629 43
Fragments arranged by size:
1629 1425 889 352 294 289 275 215
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2045 2980 1537
Size: 275 233 935 4090
Fragments arranged by size:
4090 935 275 233
EarI CTCTTCn'nnn_
Cuts at: 1353 2664 3463 1353
Size: 1311 799 3423
Fragments arranged by size:
3423 1311 799
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4120 4494 4640 387
Size: 1064 138 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2688 2751 3596 4193 4980 5248
Size: 393 474 582 63 845 597 787 268
Cuts at: 5248 1239
Size: 1524
Fragments arranged by size:
1524 845 787 597 582 474 393 268
63
EcoO109I rG'GnC_Cy
Cuts at: 2289 2543 2289
Size: 254 5279
EcoRI G'AATT_C
Cuts at: 2159 2842 3220 2159
Size: 683 378 4472
Fragments arranged by size:
4472 683 378
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2012 2392 4680 1678
Size: 334 380 2288 2531
Fragments arranged by size:
2531 2288 380 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2724 2756 4174 4206 4248
Size: 32 135 32 1152 32 1418 32 42
Cuts at: 4248 4280 1373
Size: 32 2626
Fragments arranged by size:
2626 1418 1152 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 4647 4831 1305
Size: 1191 2151 184 2007
Fragments arranged by size:
2151 2007 1191 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 10
Size: 54 1425 343 567 3144
Fragments arranged by size:
3144 1425 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3491 3850 4009 518
Size: 648 2325 359 159 2042
Fragments arranged by size:
2325 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2285 2949 3626 4774 5019 1606
Size: 438 241 664 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 438 245 241
NcoI C'CATG_G
Cuts at: 513 1065 2004 2215 513
Size: 552 939 211 3831
Fragments arranged by size:
3831 939 552 211
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2891 4436 1132
Size: 97 1662 1545 2229
Fragments arranged by size:
2229 1662 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2173 1348
Size: 825 4708
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2173 1108
Size: 1065 4468
PfoI T'CCnGG_A
Cuts at: 2545 2545
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2289 2543 2289
Size: 254 5279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2885 830
Size: 2055 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2285 2285
Size: 5533
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 5533
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 2381 3781 5493 2381
Size: 1400 1712 2421
Fragments arranged by size:
2421 1712 1400
SfcI C'TryA_G
Cuts at: 826 1045 2101 2881 4016 4697 4888 826
Size: 219 1056 780 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 780 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2141 2860 1872
Size: 269 719 4545
Fragments arranged by size:
4545 719 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 4538 4800 820
Size: 197 926 1694 901 262 1553
Fragments arranged by size:
1694 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 2675 1517
Size: 1158 4375
StyI C'CwwG_G
Cuts at: 513 1065 2004 2215 2400 2683 2972 513
Size: 552 939 211 185 283 289 3074
Fragments arranged by size:
3074 939 552 289 283 211 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 2761 3560 3577 3730 3915 4360
Size: 1255 585 596 799 17 153 185 445
Cuts at: 4360 325
Size: 1498
Fragments arranged by size:
1498 1255 799 596 585 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 2415 2786
Size: 274 80 33 51 1443 402 36 371
Cuts at: 2786 3779 5491 96
Size: 993 1712 138
Fragments arranged by size:
1712 1443 993 402 371 274 138 80
51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2174
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2139 2858 1870
Size: 269 719 4545
Fragments arranged by size:
4545 719 269
XmnI GAAnn'nnTTC
Cuts at: 3662 3662
Size: 5533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI