Restriction map for
pFN21AB6394
(Circular) (Six-base) MAPSORT of: pFN21AB6394.seq Check: 4438 from: 1 to: 5614
pFN21AB6394 5614 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2522 2522
Size: 5614
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2405 2824 2935 2405
Size: 419 111 5084
Fragments arranged by size:
5084 419 111
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3677 5061 5329 1632
Size: 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 2415 4513 5490 1203
Size: 1212 2098 977 1327
Fragments arranged by size:
2098 1327 1212 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 5582
AlwNI CAG_nnn'CTG
Cuts at: 2439 3357 4929 2439
Size: 918 1572 3124
Fragments arranged by size:
3124 1572 918
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2923 3149 3301 5410 1493
Size: 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 1870
Size: 73 996 4545
Fragments arranged by size:
4545 996 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2405 2824 2935 4390
Size: 325 217 627 251 367 419 111 1455
Cuts at: 4390 618
Size: 1842
Fragments arranged by size:
1842 1455 627 419 367 325 251 217
111
BanII G_rGCy'C
Cuts at: 1391 2118 2933 1391
Size: 727 815 4072
Fragments arranged by size:
4072 815 727
BbeI G_GCGC'C
Cuts at: 2042 2042
Size: 5614
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 4225 136
Size: 107 122 71 631 605 343 2210 1525
Fragments arranged by size:
2210 1525 631 605 343 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 2907 3031 2907
Size: 124 5490
Bme1580I G_kGCm'C
Cuts at: 1790 3675 4831 1790
Size: 1885 1156 2573
Fragments arranged by size:
2573 1885 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2740 2737
Size: 3 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2538 2628 4274 1239
Size: 902 397 90 1646 2579
Fragments arranged by size:
2579 1646 902 397 90
Bpu10I CC'TnA_GC
Cuts at: 2019 2019
Size: 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 3739 4604 4902 2408
Size: 1331 865 298 3120
Fragments arranged by size:
3120 1331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 2725 2882 4277 882
Size: 617 885 341 157 1395 2219
Fragments arranged by size:
2219 1395 885 617 341 157
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 3421 3803 4438
Size: 53 83 186 936 506 1382 382 635
Cuts at: 4438 275
Size: 1451
Fragments arranged by size:
1451 1382 936 635 506 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2247 4047 4445 4719 4866 5277 2247
Size: 1800 398 274 147 411 2584
Fragments arranged by size:
2584 1800 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2273 2343 2713 2729 1186
Size: 1084 3 70 370 16 4071
Fragments arranged by size:
4071 1084 370 70 16 3
BseYI C'CCAG_C
Cuts at: 2125 4817 5313 2125
Size: 2692 496 2426
Fragments arranged by size:
2692 2426 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3675 3760 4831 2118
Size: 1557 85 1071 2901
Fragments arranged by size:
2901 1557 1071 85
BsmI GAATG_Cn'
Cuts at: 2512 3125 3218 2512
Size: 613 93 4908
Fragments arranged by size:
4908 613 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2933 3675 3760 4831 1391
Size: 399 328 815 742 85 1071 2174
Fragments arranged by size:
2174 1071 815 742 399 328 85
BspEI T'CCGG_A
Cuts at: 2247 5277 2247
Size: 3030 2584
BspHI T'CATG_A
Cuts at: 2833 3503 5233 2833
Size: 670 1730 3214
Fragments arranged by size:
3214 1730 670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 2969 844
Size: 1678 447 3489
Fragments arranged by size:
3489 1678 447
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 4109 4283 65
Size: 1183 894 1967 174 1396
Fragments arranged by size:
1967 1396 1183 894 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 4258 4445 1700
Size: 110 265 2183 187 2869
Fragments arranged by size:
2869 2183 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 2220 3674 4686 2220
Size: 1454 1012 3148
Fragments arranged by size:
3148 1454 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2383 794
Size: 1589 4025
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2459 2944 3311 3696 3713 5165 5251 5263
Size: 487 485 367 385 17 1452 86 12
Cuts at: 5263 5609 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 487 485 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2712 2712
Size: 5614
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 513
Size: 552 939 81 4042
Fragments arranged by size:
4042 939 552 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 2584 3130 3924 3944 2584
Size: 546 794 20 4254
Fragments arranged by size:
4254 794 546 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DraIII CAC_nnn'GTG
Cuts at: 2383 2383
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 3061 3950
Size: 54 1425 50 275 18 234 997 889
Cuts at: 3950 5579 8
Size: 1629 43
Fragments arranged by size:
1629 1425 997 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 1353
Size: 2191 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2116 2116
Size: 5614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2538 2628 3677 4274 5061 5329
Size: 393 509 397 90 1049 597 787 268
Cuts at: 5329 1239
Size: 1524
Fragments arranged by size:
1524 1049 787 597 509 397 393 268
90
EcoO109I rG'GnC_Cy
Cuts at: 2860 2860
Size: 5614
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 5614
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 2042 4761 1678
Size: 364 2719 2531
Fragments arranged by size:
2719 2531 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2448 2451 2480 2483 4255
Size: 32 135 32 876 3 29 3 1772
Cuts at: 4255 4287 4329 4361 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1772 876 135 42 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2659 748
Size: 1911 3703
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KasI G'GCGC_C
Cuts at: 2038 2038
Size: 5614
KpnI G_GTAC'C
Cuts at: 2409 2828 2939 2409
Size: 419 111 5084
Fragments arranged by size:
5084 419 111
MfeI C'AATT_G
Cuts at: 2593 3213 2593
Size: 620 4994
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 4728 4912 1305
Size: 834 2589 184 2007
Fragments arranged by size:
2589 2007 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 10
Size: 54 1425 343 234 3558
Fragments arranged by size:
3558 1425 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2817 3572 3931 4090 518
Size: 648 1651 755 359 159 2042
Fragments arranged by size:
2042 1651 755 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3030 3707 4855 5100 1606
Size: 1424 677 1148 245 2120
Fragments arranged by size:
2120 1424 1148 677 245
NarI GG'CG_CC
Cuts at: 2039 2039
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4123
Fragments arranged by size:
4123 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2419 2972 4517 1132
Size: 97 1190 553 1545 2229
Fragments arranged by size:
2229 1545 1190 553 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 2415 4513 2415
Size: 2098 3516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5614
PfoI T'CCnGG_A
Cuts at: 2118 2438 2118
Size: 320 5294
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2860 2860
Size: 5614
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2055 2193 2579 2755 2966 830
Size: 1225 138 386 176 211 3478
Fragments arranged by size:
3478 1225 386 211 176 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
SacI G_AGCT'C
Cuts at: 2118 2118
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2051 2189 2575 2751 2962 4097 4778
Size: 219 1006 138 386 176 211 1135 681
Cuts at: 4778 4969 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1006 681 386 219 211 191
176 138
SfoI GGC'GCC
Cuts at: 2040 2040
Size: 5614
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 2075 1700
Size: 375 5239
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 3718 4619 4881 820
Size: 197 926 444 1331 901 262 1553
Fragments arranged by size:
1553 1331 926 901 444 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2004 2847 3053 513
Size: 552 939 843 206 3074
Fragments arranged by size:
3074 939 843 552 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3641 3658 3811 3996 4441 325
Size: 1255 2061 17 153 185 445 1498
Fragments arranged by size:
2061 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3860 5572 96
Size: 274 80 33 51 1443 1883 1712 138
Fragments arranged by size:
1883 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2581 2613 2763 2795 5574 5606 2581
Size: 32 150 32 2779 32 2589
Fragments arranged by size:
2779 2589 150 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI