Restriction map for
pFN21AB6393
(Circular) (Six-base) MAPSORT of: pFN21AB6393.seq Check: 864 from: 1 to: 5533
pFN21AB6393 5533 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2664 2876 1937
Size: 727 212 4594
Fragments arranged by size:
4594 727 212
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3596 4980 5248 1632
Size: 1964 1384 268 1917
Fragments arranged by size:
1964 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 4432 5409 1203
Size: 3229 977 1327
Fragments arranged by size:
3229 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 2104 4262 2104
Size: 2158 3375
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 2461 3276 4848 2461
Size: 815 1572 3146
Fragments arranged by size:
3146 1572 815
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2842 3068 3220 5329 1493
Size: 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2440 2636 2858 1870
Size: 73 497 196 222 4545
Fragments arranged by size:
4545 497 222 196 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2854 4309 618
Size: 325 217 627 1067 1455 1842
Fragments arranged by size:
1842 1455 1067 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2801 2852 1391
Size: 1410 51 4072
Fragments arranged by size:
4072 1410 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2600 928
Size: 971 701 3861
Fragments arranged by size:
3861 971 701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2066 2100 3724 3758 1903
Size: 34 129 34 1624 34 3678
Fragments arranged by size:
3678 1624 129 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 2331 1548
Size: 783 4750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4144 136
Size: 107 122 71 631 605 2472 1525
Fragments arranged by size:
2472 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2950 2950
Size: 5533
Bme1580I G_kGCm'C
Cuts at: 1790 3594 4750 1790
Size: 1804 1156 2573
Fragments arranged by size:
2573 1804 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 4222 442
Size: 416 53 83 399 24 1847 958 1753
Fragments arranged by size:
1847 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2551 2626 4193 1239
Size: 1244 68 75 1567 2579
Fragments arranged by size:
2579 1567 1244 75 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2339 4196 882
Size: 617 840 1857 2219
Fragments arranged by size:
2219 1857 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3340 3722 4357 275
Size: 53 83 186 936 1807 382 635 1451
Fragments arranged by size:
1807 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 1178
Size: 30 165 30 1320 30 3958
Fragments arranged by size:
3958 1320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2623 1186
Size: 1117 320 4096
Fragments arranged by size:
4096 1117 320
BseYI C'CCAG_C
Cuts at: 2551 4736 5232 2551
Size: 2185 496 2852
Fragments arranged by size:
2852 2185 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4960
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 2801 3594 3679 4750 2517
Size: 284 793 85 1071 3300
Fragments arranged by size:
3300 1071 793 284 85
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
BsmBI CGTCTCn'nnnn_
Cuts at: 2112 2112
Size: 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2517 2801 2852 3594 3679 4750 1391
Size: 399 727 284 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 727 399 284 85 51
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 2328 3422 5152 2328
Size: 1094 1730 2709
Fragments arranged by size:
2709 1730 1094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2377 2888 844
Size: 1533 511 3489
Fragments arranged by size:
3489 1533 511
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 4028 4202 65
Size: 1183 1452 1328 174 1396
Fragments arranged by size:
1452 1396 1328 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528
Size: 891 367 385 17 1452 86 12 346
Cuts at: 5528 1972
Size: 1977
Fragments arranged by size:
1977 1452 891 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2248 513
Size: 552 939 244 3798
Fragments arranged by size:
3798 939 552 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 2562 3049 3843 3863 2562
Size: 487 794 20 4232
Fragments arranged by size:
4232 794 487 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2315 4540 809
Size: 507 999 2225 1802
Fragments arranged by size:
2225 1802 999 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2980 3869
Size: 54 1425 50 275 18 405 745 889
Cuts at: 3869 5498 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 745 405 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3463 1353
Size: 2110 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 4120 4494 4640 387
Size: 1064 138 534 1997 374 146 1280
Fragments arranged by size:
1997 1280 1064 534 374 146 138
EcoICRI GAG'CTC
Cuts at: 2799 2799
Size: 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2483 2551 2626 3596 4193 4980 5248
Size: 393 851 68 75 970 597 787 268
Cuts at: 5248 1239
Size: 1524
Fragments arranged by size:
1524 970 851 787 597 393 268 75
68
EcoO109I rG'GnC_Cy
Cuts at: 2046 2266 2467 2562 2632 2046
Size: 220 201 95 70 4947
Fragments arranged by size:
4947 220 201 95 70
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 4680 1678
Size: 3002 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2723 2755 4174
Size: 32 135 32 518 32 601 32 1419
Cuts at: 4174 4206 4248 4280 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1419 601 518 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4647 4831 1305
Size: 3342 184 2007
Fragments arranged by size:
3342 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 10
Size: 54 1425 343 405 3306
Fragments arranged by size:
3306 1425 405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3491 3850 4009 518
Size: 648 2325 359 159 2042
Fragments arranged by size:
2325 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2302 2949 3626 4774 5019 1606
Size: 696 647 677 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 647 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2248 513
Size: 552 939 244 3798
Fragments arranged by size:
3798 939 552 244
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2891 4436 1132
Size: 97 1662 1545 2229
Fragments arranged by size:
2229 1662 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2424 2461 1108
Size: 1316 37 4180
Fragments arranged by size:
4180 1316 37
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2632 2467
Size: 165 5368
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2301 2628 2885 830
Size: 1471 327 257 3478
Fragments arranged by size:
3478 1471 327 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2302 2302
Size: 5533
SacI G_AGCT'C
Cuts at: 2801 2801
Size: 5533
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SexAI A'CCwGG_T
Cuts at: 2469 2469
Size: 5533
SfcI C'TryA_G
Cuts at: 826 1045 2297 2624 2881 4016 4697 4888 826
Size: 219 1252 327 257 1135 681 191 1471
Fragments arranged by size:
1471 1252 1135 681 327 257 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2860 1872
Size: 988 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 4538 4800 820
Size: 197 926 1694 901 262 1553
Fragments arranged by size:
1694 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2004 2248 2972 513
Size: 552 939 244 724 3074
Fragments arranged by size:
3074 939 724 552 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3560 3577 3730 3915 4360 325
Size: 1255 1980 17 153 185 445 1498
Fragments arranged by size:
1980 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3779 5491 96
Size: 274 80 33 51 1443 1802 1712 138
Fragments arranged by size:
1802 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2217 2249 5493 5525 2217
Size: 32 3244 32 2225
Fragments arranged by size:
3244 2225 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2858 1870
Size: 988 4545
XmnI GAAnn'nnTTC
Cuts at: 3662 3662
Size: 5533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI