Restriction map for
pFN21AB6392
(Circular) (Six-base) MAPSORT of: pFN21AB6392.seq Check: 3362 from: 1 to: 5614
pFN21AB6392 5614 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2287 2935 2287
Size: 648 4966
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3677 5061 5329 1632
Size: 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2350 2382 2350
Size: 32 5582
AlwNI CAG_nnn'CTG
Cuts at: 2119 3357 4929 2119
Size: 1238 1572 2804
Fragments arranged by size:
2804 1572 1238
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2324 2456 2923 3149 3301 5410 1493
Size: 831 132 467 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 467 226 152 132
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2404 2939 1870
Size: 73 461 535 4545
Fragments arranged by size:
4545 535 461 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 2935 4390 618
Size: 325 217 627 500 648 1455 1842
Fragments arranged by size:
1842 1455 648 627 500 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2545 2933 1391
Size: 621 533 388 4072
Fragments arranged by size:
4072 621 533 388
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 2011 3031 2011
Size: 1020 4594
Bme1580I G_kGCm'C
Cuts at: 1790 2396 2585 3675 4831 1790
Size: 606 189 1090 1156 2573
Fragments arranged by size:
2573 1156 1090 606 189
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2579 3345 4303
Size: 416 53 83 399 24 1162 766 958
Cuts at: 4303 442
Size: 1753
Fragments arranged by size:
1753 1162 958 766 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 2123 2657 4274 1239
Size: 843 41 534 1617 2579
Fragments arranged by size:
2579 1617 843 534 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2728 3739 4604 4902 2728
Size: 1011 865 298 3440
Fragments arranged by size:
3440 1011 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2058 2220 2500 4277 882
Size: 617 559 162 280 1777 2219
Fragments arranged by size:
2219 1777 617 559 280 162
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4047 4445 4719 4866 5277 4047
Size: 398 274 147 411 4384
Fragments arranged by size:
4384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5614
BseYI C'CCAG_C
Cuts at: 4817 5313 4817
Size: 496 5118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 3675 3760 4831 2538
Size: 1137 85 1071 3321
Fragments arranged by size:
3321 1137 1071 85
BsmI GAATG_Cn'
Cuts at: 2150 2441 3125 3218 2150
Size: 291 684 93 4546
Fragments arranged by size:
4546 684 291 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2396 2538 2545 2585 2933 3675
Size: 399 222 384 142 7 40 348 742
Cuts at: 3675 3760 4831 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 348 222 142
85 40 7
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2969 844
Size: 2125 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2617 2805 4109 4283 65
Size: 1183 1369 188 1304 174 1396
Fragments arranged by size:
1396 1369 1304 1183 188 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4258 4445 1700
Size: 110 2448 187 2869
Fragments arranged by size:
2869 2448 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 2464 3674 4686 2464
Size: 1210 1012 3392
Fragments arranged by size:
3392 1210 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2100 2160 2169 2643 794
Size: 1306 60 9 474 3765
Fragments arranged by size:
3765 1306 474 60 9
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2944 3311 3696 3713 5165 5251 5263 5609
Size: 972 367 385 17 1452 86 12 346
Cuts at: 5609 1972
Size: 1977
Fragments arranged by size:
1977 1452 972 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2251 513
Size: 552 939 247 3876
Fragments arranged by size:
3876 939 552 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 2585 3130 3924 3944 2585
Size: 545 794 20 4255
Fragments arranged by size:
4255 794 545 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2737 3061 3950
Size: 54 1425 50 275 18 907 324 889
Cuts at: 3950 5579 8
Size: 1629 43
Fragments arranged by size:
1629 1425 907 889 324 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 1353
Size: 2191 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2123 2657 3677 4274 5061 5329
Size: 393 450 41 534 1020 597 787 268
Cuts at: 5329 1239
Size: 1524
Fragments arranged by size:
1524 1020 787 597 534 450 393 268
41
EcoO109I rG'GnC_Cy
Cuts at: 2256 2256
Size: 5614
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 4761 1678
Size: 3083 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373
Size: 32 135 32 2683 32 42 32 2626
Fragments arranged by size:
2683 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2291 2939 2291
Size: 648 4966
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2739 10
Size: 54 1425 343 907 2885
Fragments arranged by size:
2885 1425 907 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3572 3931 4090 518
Size: 648 2406 359 159 2042
Fragments arranged by size:
2406 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2191 3030 3707 4855 5100 1606
Size: 409 176 839 677 1148 245 2120
Fragments arranged by size:
2120 1148 839 677 409 245 176
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4123
Fragments arranged by size:
4123 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2188 2489 2972 4517 1132
Size: 97 959 301 483 1545 2229
Fragments arranged by size:
2229 1545 959 483 301 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2680 1108
Size: 1572 4042
PfoI T'CCnGG_A
Cuts at: 2059 2139 2059
Size: 80 5534
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2256 2256
Size: 5614
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PspXI vC'TCGA_Gb
Cuts at: 2404 2404
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2265 2966 830
Size: 1435 701 3478
Fragments arranged by size:
3478 1435 701
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
PvuII CAG'CTG
Cuts at: 2015 2191 2015
Size: 176 5438
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SanDI GG'GwC_CC
Cuts at: 2256 2256
Size: 5614
SbfI CC_TGCA'GG
Cuts at: 2265 2966 2265
Size: 701 4913
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2261 2500 2962 4097 4778 4969 826
Size: 219 1216 239 462 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 462 239 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2404 2743 3718 4619 4881 820
Size: 197 926 461 339 975 901 262 1553
Fragments arranged by size:
1553 975 926 901 461 339 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2489 2972 1132
Size: 1357 483 3774
Fragments arranged by size:
3774 1357 483
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2004 2811 3053 513
Size: 552 939 807 242 3074
Fragments arranged by size:
3074 939 807 552 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3641 3658 3811 3996 4441 325
Size: 1255 2061 17 153 185 445 1498
Fragments arranged by size:
2061 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3860 5572
Size: 274 80 33 51 1443 204 1679 1712
Cuts at: 5572 96
Size: 138
Fragments arranged by size:
1712 1679 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2135 2167 2409 2441 2888 2920 5574 5606 2135
Size: 32 242 32 447 32 2654 32 2143
Fragments arranged by size:
2654 2143 447 242 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 2404 1943
Size: 461 5153
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI