Restriction map for
pFN21AB6390
(Circular) (Six-base) MAPSORT of: pFN21AB6390.seq Check: 9827 from: 1 to: 5608
pFN21AB6390 5608 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2798 3671 5055 5323 1632
Size: 1166 873 1384 268 1917
Fragments arranged by size:
1917 1384 1166 873 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2194 2226 2194
Size: 32 5576
AlwNI CAG_nnn'CTG
Cuts at: 3351 4923 3351
Size: 1572 4036
ApaI G_GGCC'C
Cuts at: 2044 2104 2291 2044
Size: 60 187 5361
Fragments arranged by size:
5361 187 60
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2272 2933 1870
Size: 73 243 86 661 4545
Fragments arranged by size:
4545 661 243 86 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2810 2843 2810
Size: 33 5575
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2255 2929 4384 618
Size: 325 217 627 264 204 674 1455 1842
Fragments arranged by size:
1842 1455 674 627 325 264 217 204
BanII G_rGCy'C
Cuts at: 1391 2044 2065 2104 2291 2927 1391
Size: 653 21 39 187 636 4072
Fragments arranged by size:
4072 653 636 187 39 21
BbeI G_GCGC'C
Cuts at: 2055 2259 2055
Size: 204 5404
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2891 928
Size: 971 992 3645
Fragments arranged by size:
3645 992 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2076 2110 3799 3833 1903
Size: 34 50 34 55 34 1689 34 3678
Fragments arranged by size:
3678 1689 55 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 2595 1548
Size: 1047 4561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2099 2286
Size: 107 122 71 631 605 388 39 187
Cuts at: 2286 4219 136
Size: 1933 1525
Fragments arranged by size:
1933 1525 631 605 388 187 122 107
71 39
BglII A'GATC_T
Cuts at: 2566 5603 2566
Size: 3037 2571
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2104 2121 2291 3669 4825 1790
Size: 254 60 17 170 1378 1156 2573
Fragments arranged by size:
2573 1378 1156 254 170 60 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 2482 3339
Size: 416 53 83 399 24 946 119 857
Cuts at: 3339 4297 442
Size: 958 1753
Fragments arranged by size:
1753 958 946 857 416 399 119 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2012 1056
Size: 956 4652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2170 2766 4268 1239
Size: 931 596 1502 2579
Fragments arranged by size:
2579 1502 931 596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2256 3415 3797
Size: 53 83 186 936 519 204 1159 382
Cuts at: 3797 4432 275
Size: 635 1451
Fragments arranged by size:
1451 1159 936 635 519 382 204 186
83 53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2143 2173 1178
Size: 30 165 30 740 30 4613
Fragments arranged by size:
4613 740 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5608
BseYI C'CCAG_C
Cuts at: 2097 2303 4811 5307 2097
Size: 206 2508 496 2398
Fragments arranged by size:
2508 2398 496 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5035
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 2166 2247 3058 3819 4847
Size: 275 186 77 88 81 811 761 1028
Cuts at: 4847 1540
Size: 2301
Fragments arranged by size:
2301 1028 811 761 275 186 88 81
77
BsiHKAI G_wGCw'C
Cuts at: 2300 2506 3669 3754 4825 2300
Size: 206 1163 85 1071 3083
Fragments arranged by size:
3083 1163 1071 206 85
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2065 2104 2121 2291 2300 2506
Size: 399 254 21 39 17 170 9 206
Cuts at: 2506 2927 3669 3754 4825 1391
Size: 421 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 254 206 170
85 39 21 17 9
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 844
Size: 2119 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2168 2252 3495 1394
Size: 429 345 84 1243 3507
Fragments arranged by size:
3507 1243 429 345 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 2024 1925
Size: 99 5509
BssSI C'ACGA_G
Cuts at: 2032 3668 4680 2032
Size: 1636 1012 2960
Fragments arranged by size:
2960 1636 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2566 2938 3305 3690 3707 5159 5245 5257
Size: 594 372 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 594 385 372 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2321 513
Size: 552 1256 3800
Fragments arranged by size:
3800 1256 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2700 3124 3918 3938 2700
Size: 424 794 20 4370
Fragments arranged by size:
4370 794 424 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 2139 2163
Size: 54 1425 50 275 18 245 64 24
Cuts at: 2163 2194 2244 2278 2878 3055 3944 5573 8
Size: 31 50 34 600 177 889 1629 43
Fragments arranged by size:
1629 1425 889 600 275 245 177 64
54 50 50 43 34 31 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2075 2163 2244 3055 1537
Size: 275 263 88 81 811 4090
Fragments arranged by size:
4090 811 275 263 88 81
EarI CTCTTCn'nnn_
Cuts at: 1353 2658 3538 1353
Size: 1305 880 3423
Fragments arranged by size:
3423 1305 880
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2170 2766 2798 3671 4268 5055 5323
Size: 393 538 596 32 873 597 787 268
Cuts at: 5323 1239
Size: 1524
Fragments arranged by size:
1524 873 787 597 596 538 393 268
32
EcoO109I rG'GnC_Cy
Cuts at: 2040 2041 2101 2287 2040
Size: 1 60 186 5361
Fragments arranged by size:
5361 186 60 1
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2508 2508
Size: 5608
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2055 2259 4755 1678
Size: 377 204 2496 2531
Fragments arranged by size:
2531 2496 377 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2175 4249 4281 4323
Size: 32 135 32 571 32 2074 32 42
Cuts at: 4323 4355 1373
Size: 32 2626
Fragments arranged by size:
2626 2074 571 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KasI G'GCGC_C
Cuts at: 2051 2255 2051
Size: 204 5404
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 2681 4722 4906 1305
Size: 1125 251 2041 184 2007
Fragments arranged by size:
2041 2007 1125 251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2243 2261 2849 3024 3701 4849 5094
Size: 594 43 18 588 175 677 1148 245
Cuts at: 5094 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 594 588 245 175 43
18
NarI GG'CG_CC
Cuts at: 2052 2256 2052
Size: 204 5404
NcoI C'CATG_G
Cuts at: 513 1065 2321 513
Size: 552 1256 3800
Fragments arranged by size:
3800 1256 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 2008 1052
Size: 956 4652
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PfoI T'CCnGG_A
Cuts at: 2065 2065
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2040 2100 2287 2040
Size: 60 187 5361
Fragments arranged by size:
5361 187 60
PstI C_TGCA'G
Cuts at: 830 2960 830
Size: 2130 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2849 2849
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2956 4091 4772 4963 826
Size: 219 1911 1135 681 191 1471
Fragments arranged by size:
1911 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2286 2286
Size: 5608
SfoI GGC'GCC
Cuts at: 2053 2257 2053
Size: 204 5404
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2188 2274 2935 1872
Size: 316 86 661 4545
Fragments arranged by size:
4545 661 316 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4613 4875 820
Size: 197 926 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 2407 3532 5
Size: 47 2355 1125 2081
Fragments arranged by size:
2355 2081 1125 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2321 3047 513
Size: 552 1256 726 3074
Fragments arranged by size:
3074 1256 726 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3635 3652 3805 3990 4435 325
Size: 1255 2055 17 153 185 445 1498
Fragments arranged by size:
2055 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2186 2272 2933 1870
Size: 316 86 661 4545
Fragments arranged by size:
4545 661 316 86
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SrfI SwaI XcmI