Restriction map for
pFN21AB6388
(Circular) (Six-base) MAPSORT of: pFN21AB6388.seq Check: 9057 from: 1 to: 5608
pFN21AB6388 5608 bp
With 182 enzymes: SgfI *
October 18, 2013 12:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2043 2951 1937
Size: 106 908 4594
Fragments arranged by size:
4594 908 106
Acc65I G'GTAC_C
Cuts at: 2843 2929 2843
Size: 86 5522
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 3671 5055 5323 1632
Size: 662 1377 1384 268 1917
Fragments arranged by size:
1917 1384 1377 662 268
AfeI AGC'GCT
Cuts at: 2663 2663
Size: 5608
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 2667 4507 5484 1203
Size: 1464 1840 977 1327
Fragments arranged by size:
1840 1464 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 2490 3351 4923 2490
Size: 861 1572 3175
Fragments arranged by size:
3175 1572 861
ApaI G_GGCC'C
Cuts at: 2442 2442
Size: 5608
ApaLI G'TGCA_C
Cuts at: 2603 3665 4821 2603
Size: 1062 1156 3390
Fragments arranged by size:
3390 1156 1062
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2407 2933 1870
Size: 73 137 327 526 4545
Fragments arranged by size:
4545 526 327 137 73
BamHI G'GATC_C
Cuts at: 2493 2938 3305 2493
Size: 445 367 4796
Fragments arranged by size:
4796 445 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2411 2843 2929 4384
Size: 325 217 627 495 129 432 86 1455
Cuts at: 4384 618
Size: 1842
Fragments arranged by size:
1842 1455 627 495 432 325 217 129
86
BanII G_rGCy'C
Cuts at: 1391 2442 2646 2861 2927 1391
Size: 1051 204 215 66 4072
Fragments arranged by size:
4072 1051 215 204 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2285 2414 2442 2607 3669 4825 1790
Size: 495 129 28 165 1062 1156 2573
Fragments arranged by size:
2573 1156 1062 495 165 129 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2436 3339 4297
Size: 416 53 83 399 24 1019 903 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1019 958 903 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2822
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2369 2552 2817 4268 1239
Size: 998 132 183 265 1451 2579
Fragments arranged by size:
2579 1451 998 265 183 132
Bpu10I CC'TnA_GC
Cuts at: 2693 2693
Size: 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2668 3399 493
Size: 711 1464 731 2702
Fragments arranged by size:
2702 1464 731 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2507 2597 3415 3797
Size: 53 83 186 936 974 90 818 382
Cuts at: 3797 4432 275
Size: 635 1451
Fragments arranged by size:
1451 974 936 818 635 382 186 90
83 53
BsaWI w'CCGG_w
Cuts at: 2861 4041 4439 4713 4860 5271 2861
Size: 1180 398 274 147 411 3198
Fragments arranged by size:
3198 1180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 2525 2555 1178
Size: 30 165 30 939 30 153 30 4231
Fragments arranged by size:
4231 939 165 153 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 1186
Size: 1123 4485
BseYI C'CCAG_C
Cuts at: 2483 4811 5307 2483
Size: 2328 496 2784
Fragments arranged by size:
2784 2328 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 2213 1163
Size: 573 275 202 4558
Fragments arranged by size:
4558 573 275 202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2894 3058 3819 4847 1540
Size: 275 186 893 164 761 1028 2301
Fragments arranged by size:
2301 1028 893 761 275 186 164
BsiHKAI G_wGCw'C
Cuts at: 2607 3669 3754 4825 2607
Size: 1062 85 1071 3390
Fragments arranged by size:
3390 1071 1062 85
BsmI GAATG_Cn'
Cuts at: 2756 3119 3212 2756
Size: 363 93 5152
Fragments arranged by size:
5152 363 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2414 2442 2607 2646 2861 2927
Size: 399 495 129 28 165 39 215 66
Cuts at: 2927 3669 3754 4825 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 399 215 165 129
85 66 39 28
BspEI T'CCGG_A
Cuts at: 2861 5271 2861
Size: 2410 3198
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2564 2963 844
Size: 1720 399 3489
Fragments arranged by size:
3489 1720 399
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2373 2846 2893 4252 4439 1700
Size: 110 563 473 47 1359 187 2869
Fragments arranged by size:
2869 1359 563 473 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 2054 3668 4680 2054
Size: 1614 1012 2982
Fragments arranged by size:
2982 1614 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2025 794
Size: 1231 4377
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 2551 2740 1184
Size: 1367 189 4052
Fragments arranged by size:
4052 1367 189
BstXI CCAn_nnnn'nTGG
Cuts at: 2530 2530
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2493 2938 3305 3690 3707 5159 5245 5257
Size: 521 445 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 521 445 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2628 513
Size: 552 1563 3493
Fragments arranged by size:
3493 1563 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3918 3938 3124
Size: 794 20 4794
Fragments arranged by size:
4794 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2891 3055 3944
Size: 54 1425 50 275 18 1061 164 889
Cuts at: 3944 5573 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1061 889 275 164 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2891 3055 1537
Size: 275 1079 164 4090
Fragments arranged by size:
4090 1079 275 164
EarI CTCTTCn'nnn_
Cuts at: 1353 3538 1353
Size: 2185 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2294 2369 2552 2817 3671 4268
Size: 393 605 57 75 183 265 854 597
Cuts at: 4268 5055 5323 1239
Size: 787 268 1524
Fragments arranged by size:
1524 854 787 605 597 393 268 265
183 75 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2351 2351
Size: 5608
EcoO109I rG'GnC_Cy
Cuts at: 2091 2401 2450 2091
Size: 310 49 5249
Fragments arranged by size:
5249 310 49
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 2582 4114 2582
Size: 1532 4076
HaeII r_GCGC'y
Cuts at: 1678 2665 4755 1678
Size: 987 2090 2531
Fragments arranged by size:
2531 2090 987
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 2525 2557 4249
Size: 32 135 32 770 32 151 32 1692
Cuts at: 4249 4281 4323 4355 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1692 770 151 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2847 2933 2847
Size: 86 5522
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2455 2568 4722 4906 1305
Size: 1150 113 2154 184 2007
Fragments arranged by size:
2154 2007 1150 184 113
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2825 3566 3925 4084 518
Size: 648 1659 741 359 159 2042
Fragments arranged by size:
2042 1659 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2278 2314 2836 3024 3701 4849 5094
Size: 469 203 36 522 188 677 1148 245
Cuts at: 5094 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 522 469 245 203 188
36
NaeI GCC'GGC
Cuts at: 2375 2375
Size: 5608
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5056
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NgoMIV G'CCGG_C
Cuts at: 2373 2373
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2587 2966 4511 1132
Size: 97 1358 379 1545 2229
Fragments arranged by size:
2229 1545 1358 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2490 1108
Size: 1382 4226
PfoI T'CCnGG_A
Cuts at: 2214 2346 2214
Size: 132 5476
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 2450 2091
Size: 359 5249
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2438 2438
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2157 2835 2960 830
Size: 1327 678 125 3478
Fragments arranged by size:
3478 1327 678 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SanDI GG'GwC_CC
Cuts at: 2091 2091
Size: 5608
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2153 2831 2956 4091 4772 4963 826
Size: 219 1108 678 125 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 681 678 219 191 125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4613 4875 820
Size: 197 926 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 2264 1517
Size: 747 4861
StyI C'CwwG_G
Cuts at: 513 1065 2397 2517 3047 513
Size: 552 1332 120 530 3074
Fragments arranged by size:
3074 1332 552 530 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3635 3652 3805 3990 4435 325
Size: 1255 2055 17 153 185 445 1498
Fragments arranged by size:
2055 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 3854 5566
Size: 274 80 33 51 1443 243 1634 1712
Cuts at: 5566 96
Size: 138
Fragments arranged by size:
1712 1634 1443 274 243 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 2129 3737 2129
Size: 1608 4000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI