Restriction map for
pFN21AB6385
(Circular) (Six-base) MAPSORT of: pFN21AB6385.seq Check: 2873 from: 1 to: 5524
pFN21AB6385 5524 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 2867 1937
Size: 493 437 4594
Fragments arranged by size:
4594 493 437
Acc65I G'GTAC_C
Cuts at: 2388 2845 2388
Size: 457 5067
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2368 3587 4971 5239 1632
Size: 398 338 1219 1384 268 1917
Fragments arranged by size:
1917 1384 1219 398 338 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 2077 2319 4423 5400 1203
Size: 874 242 2104 977 1327
Fragments arranged by size:
2104 1327 977 874 242
AgeI A'CCGG_T
Cuts at: 2541 4355 2541
Size: 1814 3710
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 2403 1166
Size: 1237 4287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 2067
Size: 32 5492
AlwNI CAG_nnn'CTG
Cuts at: 2581 3267 4839 2581
Size: 686 1572 3266
Fragments arranged by size:
3266 1572 686
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2086 2849 1870
Size: 73 85 58 763 4545
Fragments arranged by size:
4545 763 85 73 58
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2388 2666 2845 4300
Size: 325 217 627 378 223 278 179 1455
Cuts at: 4300 618
Size: 1842
Fragments arranged by size:
1842 1455 627 378 325 278 223 217
179
BanII G_rGCy'C
Cuts at: 1391 2028 2094 2343 2507 2627 2693 2706 2843
Size: 637 66 249 164 120 66 13 137
Cuts at: 2843 1391
Size: 4072
Fragments arranged by size:
4072 637 249 164 137 120 66 66
13
BbeI G_GCGC'C
Cuts at: 2169 2670 2169
Size: 501 5023
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 928
Size: 971 645 3908
Fragments arranged by size:
3908 971 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3715 3749 1903
Size: 34 50 34 1694 34 3678
Fragments arranged by size:
3678 1694 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 2652 1548
Size: 1104 4420
BfrBI ATG'CAT
Cuts at: 2066 2066
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4135 136
Size: 107 122 71 631 605 2463 1525
Fragments arranged by size:
2463 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 3585 4741 1790
Size: 1795 1156 2573
Fragments arranged by size:
2573 1795 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2280 2585 2804 4184 1239
Size: 803 238 305 219 1380 2579
Fragments arranged by size:
2579 1380 803 305 238 219
Bpu10I CC'TnA_GC
Cuts at: 2574 2574
Size: 5524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 3649 4514 4812 2078
Size: 1571 865 298 2790
Fragments arranged by size:
2790 1571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4187 882
Size: 617 2688 2219
Fragments arranged by size:
2688 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2401 3315 493
Size: 711 1197 914 2702
Fragments arranged by size:
2702 1197 914 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2274 2300 2667
Size: 53 83 186 936 633 108 26 367
Cuts at: 2667 3331 3713 4348 275
Size: 664 382 635 1451
Fragments arranged by size:
1451 936 664 635 633 382 367 186
108 83 53 26
BsaWI w'CCGG_w
Cuts at: 2541 3957 4355 4629 4776 5187 2541
Size: 1416 398 274 147 411 2878
Fragments arranged by size:
2878 1416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2067 2097 2609 2639 1178
Size: 30 165 30 664 30 512 30 4063
Fragments arranged by size:
4063 664 512 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5524
BseYI C'CCAG_C
Cuts at: 4727 5223 4727
Size: 496 5028
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 3862
Fragments arranged by size:
3862 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 2974 3735 4763 1540
Size: 275 186 195 778 761 1028 2301
Fragments arranged by size:
2301 1028 778 761 275 195 186
BsiHKAI G_wGCw'C
Cuts at: 2693 3585 3670 4741 2693
Size: 892 85 1071 3476
Fragments arranged by size:
3476 1071 892 85
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2094 2343 2507 2627 2693 2706
Size: 399 238 66 249 164 120 66 13
Cuts at: 2706 2843 3585 3670 4741 1391
Size: 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 249 238 164 137
120 85 66 66 13
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2641 4019 4193 65
Size: 1183 1393 1378 174 1396
Fragments arranged by size:
1396 1393 1378 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2541 4168 4355 1700
Size: 110 731 1627 187 2869
Fragments arranged by size:
2869 1627 731 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 2615 1184
Size: 1431 4093
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2293
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2220 2607 2854 3221 3606 3623 5075 5161
Size: 248 387 247 367 385 17 1452 86
Cuts at: 5161 5173 5519 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 387 385 367 346 248 247
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2268 2352 2467 513
Size: 552 1203 84 115 3570
Fragments arranged by size:
3570 1203 552 115 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2113 2215 2394 487
Size: 683 56 887 102 179 3617
Fragments arranged by size:
3617 887 683 179 102 56
BtsI GCAGTG_nn'
Cuts at: 3040 3834 3854 3040
Size: 794 20 4710
Fragments arranged by size:
4710 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2719 2971
Size: 54 1425 50 275 18 519 370 252
Cuts at: 2971 3860 5489 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 519 370 275 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 2705 3454 1353
Size: 1034 318 749 3423
Fragments arranged by size:
3423 1034 749 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 4111 4485 4631 387
Size: 1064 138 627 1895 374 146 1280
Fragments arranged by size:
1895 1280 1064 627 374 146 138
EcoICRI GAG'CTC
Cuts at: 2691 2691
Size: 5524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2042 2280 2368 2585 2804 3587
Size: 393 398 12 238 88 217 219 783
Cuts at: 3587 4184 4971 5239 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 398 393 268 238
219 217 88 12
EcoO109I rG'GnC_Cy
Cuts at: 2098 2098
Size: 5524
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2169 2670 4671 1678
Size: 491 501 2001 2531
Fragments arranged by size:
2531 2001 501 491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2433 2465 2609 2641 4165
Size: 32 135 32 861 32 144 32 1524
Cuts at: 4165 4197 4239 4271 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1524 861 144 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KasI G'GCGC_C
Cuts at: 2165 2666 2165
Size: 501 5023
KpnI G_GTAC'C
Cuts at: 2392 2849 2392
Size: 457 5067
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 2077 2319 5400 2077
Size: 242 3081 2201
Fragments arranged by size:
3081 2201 242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 10
Size: 54 1425 343 519 3183
Fragments arranged by size:
3183 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2403 2466 3482 3841 4000 518
Size: 648 1125 112 63 1016 359 159 2042
Fragments arranged by size:
2042 1125 1016 648 359 159 112 63
MspA1I CmG'CkG
Cuts at: 1606 2270 2750 2940 3617 4765 5010 1606
Size: 664 480 190 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 480 245 190
NarI GG'CG_CC
Cuts at: 2166 2667 2166
Size: 501 5023
NcoI C'CATG_G
Cuts at: 513 1065 2467 513
Size: 552 1402 3570
Fragments arranged by size:
3570 1402 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NsiI A_TGCA'T
Cuts at: 2068 2068
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2649 2882 4427 1132
Size: 97 1420 233 1545 2229
Fragments arranged by size:
2229 1545 1420 233 97
PasI CC'CwG_GG
Cuts at: 1348 2500 1348
Size: 1152 4372
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PmlI CAC'GTG
Cuts at: 2401 2401
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2067 2099 3593 3625 5106 5138 2067
Size: 32 1494 32 1481 32 2453
Fragments arranged by size:
2453 1494 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2098
Size: 5524
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
PvuII CAG'CTG
Cuts at: 2750 2750
Size: 5524
SacI G_AGCT'C
Cuts at: 2693 2693
Size: 5524
SacII CC_GC'GG
Cuts at: 2271 2271
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SapI GCTCTTCn'nnn_
Cuts at: 2387 2387
Size: 5524
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SfcI C'TryA_G
Cuts at: 826 1045 2476 2872 4007 4688 4879 826
Size: 219 1431 396 1135 681 191 1471
Fragments arranged by size:
1471 1431 1135 681 396 219 191
SfoI GGC'GCC
Cuts at: 2167 2668 2167
Size: 501 5023
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2088 2851 1872
Size: 216 763 4545
Fragments arranged by size:
4545 763 216
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2093 3628 4529 4791 820
Size: 197 926 150 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197 150
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5524
StyI C'CwwG_G
Cuts at: 513 1065 2467 2963 513
Size: 552 1402 496 3074
Fragments arranged by size:
3074 1402 552 496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2182 2393 2603 2710 3551 3568 3721
Size: 1255 602 211 210 107 841 17 153
Cuts at: 3721 3906 4351 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 841 602 445 211 210 185
153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3770 5482 96
Size: 274 80 33 51 1443 1793 1712 138
Fragments arranged by size:
1793 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2599 2631 5484 5516 2599
Size: 32 2853 32 2607
Fragments arranged by size:
2853 2607 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2153 1316
Size: 837 4687
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2293 2735 2134
Size: 159 442 4923
Fragments arranged by size:
4923 442 159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2086 2849 1870
Size: 216 763 4545
Fragments arranged by size:
4545 763 216
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI
RsrII SanDI SexAI SfiI SrfI SwaI